The Effect of Nutritional Interventions on Exercise-induced Muscle Damage

The study is a randomized, double-blinded, placebo-controlled trial that will include the participation of 69 women, aged 18-40 years. The aim of the study is to test nutritional strategies that help to minimize the effects of muscle damage induced by exercise.

The procedures will be performed at the Federal University of Health of Porto Alegre (UFCSPA).

Study Overview

• Status: Completed
• Conditions: Muscle Damage
• Intervention / Treatment: Dietary supplement: Omega 3; Other: Eccentric exercise; Dietary supplement: Isolate whey protein; Dietary supplement: Omega 3 and Isolate whey protein

Detailed Description

The exercise-induced muscle damage is associated with eccentric activities affecting muscle structural unit through micro-injury that can promote a local inflammatory response, muscle damage and delayed onset muscle soreness. These events can cause an impact on athletic performance and lower adherence to the training programs. To optimize muscle recovery after the damage caused by exercise is essential to ensure adequate caloric intake , with required amounts of macronutrients and micronutrients. Recent studies have been showed that the protein intake before sleeping is a effective strategy to inhibit muscle protein degradation, stimulate muscle protein synthesis and facilitate adaptive response of skeletal muscle to physical exercise. Furthermore, evidences on the use of anti-inflammatory nutrients have suggested that diets rich in omega 3 can reduce inflammation through inhibition of pro-inflammatory mediators. Therefore, the intake of this nutrient combined with a protein meal before sleeps and the control of food intake can improve adherence to sports training. The study will consist of three groups where everyone will receive an individualized diet. A randomization procedure will determine the type of intervention. The distribution of the groups will be as follows: group 1 (control) - volunteers follow individualized diet and olive oil capsules and maltodextrin; group 2 - intervention with omega 3 more individualized diet ; group 3 - omega 3 and whey protein isolate intervention more individualized diet.

The order of procedures to be performed will be: (1) Nutritional and Anthropometric assessment; (2) Individual orientation of diet and distribution of supplements; (3) Baseline assessment (peak torque, muscle pain levels and vastus lateralis and rectus femoris echo intensity; (4) Muscle damage induction by eccentric exercise protocol; (5) Reviews after 24h, 48h, 72h, which will follow the routine and the methods applied at baseline.

• Study Type: Interventional
• Enrollment (Actual): 2
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Brazil
  • Rio Grande do Sul
    • Porto Alegre, Rio Grande do Sul, Brazil, 90050-170
      • UFCSPA

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 40 years (ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Female

Description

Inclusion Criteria:

• 18 - 40 years aged
• BMI 18,5-25kg/m2
• physical exercise: < 150 minutes per week

Exclusion Criteria:

• pregnancy/ lactation
• vegetarian/vegan
• chronic disease
• pulmonary and neurologic disease
• muscle injury/knee muscle injury
• medication use: anti-inflammatory and anticoagulant
• fish oil and derivate allergy
• dietary supplements use in the last month: whey protein, casein, albumin, glutamin, meat and soy protein, creatine, branched chain amino acid, high calorie supplement, n-3 supplement and antioxidants

Study Plan

How is the study designed?

Design Details

• Primary Purpose: PREVENTION
• Allocation: RANDOMIZED
• Interventional Model: PARALLEL
• Masking: DOUBLE

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
PLACEBO_COMPARATOR: Omega 3; 3000mg of olive oil; Eccentric exercise: 100 maximal eccentric contractions of knee extensors, divided into 10 sets of 10 repetitions at intervals of 60 seconds between the series	Dietary supplement: Omega 3; 3000mg of n-3 oil (2000mg EPA e 1000mg DHA); Other: Eccentric exercise; 100 maximal eccentric contractions of knee extensors, divided into 10 sets of 10 repetitions at intervals of 60 seconds between the series
PLACEBO_COMPARATOR: Isolate whey protein; 23g of maltodextrin; Eccentric exercise: 100 maximal eccentric contractions of knee extensors, divided into 10 sets of 10 repetitions at intervals of 60 seconds between the series	Other: Eccentric exercise; 100 maximal eccentric contractions of knee extensors, divided into 10 sets of 10 repetitions at intervals of 60 seconds between the series; Dietary supplement: Isolate whey protein; 23g of protein of whey protein
PLACEBO_COMPARATOR: Omega 3 and Isolate whey protein; 3000mg of olive oil and 23g of maltodextrin; Eccentric exercise: 100 maximal eccentric contractions of knee extensors, divided into 10 sets of 10 repetitions at intervals of 60 seconds between the series	Other: Eccentric exercise; 100 maximal eccentric contractions of knee extensors, divided into 10 sets of 10 repetitions at intervals of 60 seconds between the series; Dietary supplement: Omega 3 and Isolate whey protein; 3000mg of n-3 oil (2000mg EPA e 1000mg DHA) and 23g of protein of whey protein

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Quadriceps muscle strength evaluation (Peak Torque)	Three maximal isometric contractions of knee extensors will be performed by the isokinetic dynamometer equipment Biodex System 4 Pro (Biodex Medical Systems, USA).	One Year

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Evaluation of quadriceps muscle pain (Muscle pain levels)	The assessment of pain as muscle damage marker is expressed by a visual analog scale.	One year
Vastus lateralis and rectus femoris echo intensity	Ultrasound images	One year

Collaborators and Investigators

• Sponsor: Federal University of Health Science of Porto Alegre
• Investigators: Principal Investigator: Claudia Dornelles Schneider, Doctor, Federal University of Health Science of Porto Alegre

Study record dates

Study Major Dates

• Study Start (ACTUAL): July 26, 2016
• Primary Completion (ACTUAL): January 9, 2017
• Study Completion (ACTUAL): February 9, 2017

Study Registration Dates

• First Submitted: July 13, 2016
• First Submitted That Met QC Criteria: July 18, 2016
• First Posted (ESTIMATE): July 21, 2016

Study Record Updates

• Last Update Posted (ACTUAL): March 9, 2017
• Last Update Submitted That Met QC Criteria: March 8, 2017
• Last Verified: March 1, 2017

More Information

Terms related to this study

• Keywords: Dietary supplement; Eccentric exercise; Muscle damage
• Other Study ID Numbers: 51384015.5.0000.5345

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: YES