House Dust Mite Induced Inflammasome Activation on Corticosteroid Resistance

July 27, 2021 updated by: China Medical University Hospital

Effects and Mechanism of House Dust Mite Induced Inflammasome Activation on Corticosteroid Resistance

In this project, PBMCs will be isolated from HDM allergic and non allergic patient and then stimulated with HDM crude extract. The expression of inflammasome and the correlation of inflammasome activation and steroid resistance will be investigated.

Study Overview

Status

Withdrawn

Conditions

Intervention / Treatment

Study Type

Interventional

Phase

  • Not Applicable

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

20 years to 70 years (Adult, Older Adult)

Accepts Healthy Volunteers

Yes

Genders Eligible for Study

All

Description

Inclusion Criteria:

  • HDM allergic and non allergic subjects

Exclusion Criteria:

  • Pregnant subject, cancer

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Basic Science
  • Allocation: Randomized
  • Interventional Model: Parallel Assignment
  • Masking: None (Open Label)

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Experimental: Steroid resistance in HDM allergic patients
Biological: House dust mite
As a stimulant to induce inflammasome activation
Experimental: Steroid resistance in non HDM allergic subjects
Biological: House dust mite
As a stimulant to induce inflammasome activation

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
inflammasome activation by HDM
Time Frame: 6 hours
The expression of NLRP3 in PBMCs will be measured by western blot after HDM stimulation.
6 hours
Steroid receptor phosphorylation by HDM
Time Frame: 6 hours
The expression of steroid receptor will be detected by western blot and real-time PCR after HDM stimulation.
6 hours

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

China Medical University Hospital

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Anticipated)

November 30, 2017

Primary Completion (Anticipated)

September 6, 2018

Study Completion (Anticipated)

September 6, 2018

Study Registration Dates

First Submitted

September 26, 2017

First Submitted That Met QC Criteria

October 5, 2017

First Posted (Actual)

October 11, 2017

Study Record Updates

Last Update Posted (Actual)

August 2, 2021

Last Update Submitted That Met QC Criteria

July 27, 2021

Last Verified

July 1, 2021

More Information

Terms related to this study

Other Study ID Numbers

  • CMUH106-REC3-115

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

UNDECIDED

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

Clinical Trials on Allergic Asthma

Clinical Trials on House dust mite

Search Similar Trials

Sponsors and Collaborators

Medical Conditions

Drug Interventions

CROs by country

CROs in Togo

Conditions

Rare Diseases

Drug Interventions

Dietary Supplements

Sponsor/Collaborators

Locations

3
Subscribe