Lung Function Variability in Children and Adolescents (LUV)

Long-term variability analysis of peak expiratory flow (PEF) and forced expiratory volume in 1 s (FEV1) has been successfully used in research to predict the exacerbation of the disease in adult individuals with asthma. However, there is a paucity of data regarding PEF and FEV1 variability in asthmatic children and adolescents. Such a task requires at least daily PEF and FEV1 measurements, recording in diaries, and periodic evaluation of the data. The process may be proven both complicated and time consuming, thus reducing patients' adherence. Recent advances in biosensor technology have permitted the development of reliable, low-cost, portable spirometers, able to connect with smartphones and monitor lung function parameters in real time and from a distance.

The objectives of the present study is the assessment of PEF and FEV1 variability: a) in healthy children and adolescents, in order to define the normal daily fluctuation of PEF and FEV1 and the parameters that may influence it, and b) in children and adolescents with asthma, in order to explore the differences from healthy subjects and reveal any specific variability changes prior to exacerbation. Such data would improve our understanding regarding the disease and permit the development of integrated tools for assessing the level of asthma control and the risk of future exacerbations.

The study will include 100 healthy children and adolescents aged 6 to 18 years for the assessment of normal PEF and FEV1 variability, and 100 children and adolescents of the same age with diagnosed asthma for the assessment of PEF and FEV1 variability in asthmatics. PEF and FEV1 measurements will be performed using an FDA-approved portable spirometer (MIR Spirobank Smart) capable to connect to smartphone. Each participant will receive his personal spirometer. Measurements will be performed twice a day between 07:00-09:00 and 19:00-21:00 hours and dispatched via email to a central database for a period of 3 months. PEF and FEV1 variability will be assessed by detrended fluctuation analysis, aiming to define the normal pattern (healthy controls) and to detect and quantify the deviations (asthmatics). The anticipated duration of the study is 24 months.

Study Overview

• Status: Recruiting
• Conditions: Asthma in Children; Lung Function Decreased
• Intervention / Treatment: Diagnostic test: Measurements of PEF and FEV1

Detailed Description

Background

Asthma represents the most common chronic disease of childhood and adolescence and an important cause of morbidity worldwide. The disease is characterized by episodes of reversible airway obstruction (exacerbations), associated with specific symptoms (wheeze, dyspnea, cough, chest tightness) and decrease in peak expiratory flow (PEF) and forced expiratory volume at 1 sec (FEV1). However, these spirometric changes occur in parallel with clinical deterioration, thus presenting limited ability to predict the exacerbation of the disease.

Both PEF and FEV1 demonstrate significant daily variability, i.e., circadian and/or day-by-day fluctuation of the measured values. In healthy individuals the pattern of these fluctuations remains constant over long time periods (weeks, months), as opposed to asthmatic patients where PEF and FEV1 variability is increased, especially prior to exacerbations when the control of the disease is lost. Thus, the analysis of PEF and FEV1 variability has been used in research to evaluate the effectiveness of treatment, recognize high-risk patients and predict asthma exacerbations.

In clinical practice, however, the evaluation of PEF and FEV1 variability requires at least daily measurements with special devices, recording in diaries, and periodic evaluation of the data by the attending physicians. The process may be proven both complicated and time consuming, thus reducing patients' adherence especially in the case of asthmatic children and adolescents. In addition, the periodic review of measurements may hamper the prediction of exacerbation, as the time of evaluation may not coincide with changes in the variability of lung function that characterize the loss of asthma control.

Over the last years, technological advancements in the field of biosensors and microprocessors have permitted the development of reliable, low-cost, portable spirometers, able to connect with cutting-edge mobile phones (smartphones) and monitor lung function parameters in real time and from a distance. The use of such devices may overcome most of the aforementioned barriers in following-up lung function parameters in the long term.

Currently, there is a paucity of data regarding PEF and FEV1 variability in children and adolescents with asthma. Such data would improve our understanding regarding the disease and permit the development of integrated tools for assessing the level of asthma control and the risk of future exacerbations

Objectives

The objectives of the study are:

1. Assessment of PEF and FEV1 variability in healthy children and adolescents: definition of the normal range of daily PEF and FEV1 fluctuation and the parameters that may influence it

2. Assessment of PEF and FEV1 variability in children and adolescents with asthma: differences from healthy subjects and definition of specific changes prior to exacerbation

   Methods

   A. Study population

   The study will include two cohorts:

   1. Healthy children and adolescents aged 6 to 18 years (N=100) for the assessment of normal PEF and FEV1 variability (objective #1).
   2. Children and adolescents aged 6 to 18 years with diagnosed asthma [2] (N=100) for the assessment of PEF and FEV1 variability in asthmatics (objective #2).

   B. Lung function measurements

   a. Device: PEF and FEV1 measurements will be performed using an FDA-approved portable spirometer (Spirobank Smart, MIR, Rome, Italy), with a disposable bi-directional digital turbine (flow range ±16L/s, volume accuracy ±3% or 50 mL, flow accuracy: ±5% or 200 mL/s, dynamic resistance <0.5 cm H2O/L/s), capable to connect to smartphone via Bluetooth® using a dedicated freeware application (iSpirometry, MIR, Rome, Italy). Each participant will receive his personal spirometer. Detailed information regarding the use and maintenance of the device will be provided (brochure & online resources).

   b. Measurements: The correct technique for PEF and FEV1 measurements (ERS/ATS standards) will be demonstrated by one of the investigators at enrollment. Detailed information regarding the technique (brochure & online resources) will be also provided. Measurements will be performed twice a day between 07:00-09:00 and 19:00-21:00 hours, for a period of 3 months.

   c. Completed measurements (encrypted pdf format) will be dispatched via email to a central database by the participants or their parents.

   d. Monitoring: Participants' adherence will be monitored continuously. The investigators will notify the participants in case of lost measurements or inappropriate technique.

   C. Variability analysis

   PEF and FEV1 variability will be assessed by:

   a. The coefficient of variation b. Detrended Fluctuation Analysis (DFA), a method that has been widely used for the investigation of intrinsic correlation within time series.14 Initially, the square root of the time series [F(n)] is calculated for segments of different (time) length n. A linear relationship in the logarithmic graph F (n) - log (n) indicates the existence of fractal architecture in the scaling of the specific data, while the slope a of the line describes the pattern of long-term fluctuations.14 A change in daily variability of PEF or FEV1 results in a simultaneous deviation from the predetermined a value.6 This deviation can easily be detected and quantified. It has been shown that the magnitude of a deviation reflects the likelihood of asthma exacerbation within the next month.6 c. Variability analysis will be performed in MatLab (MathWorks, Inc., Natick, MA, USA) environment.

   D. Additional data Patients' characteristics (age, sex, baseline lung function, allergy, comorbidities, medication, etc) will be also recorded. These parameters will be included in regression models to explore their effect on the pattern of PEF and FEV1 variability.

   E. Statistics All analyses will be performed with the IBM SPSS software version 25.0 (IBM Corp., Armonk, NY).

   F. Timeframes The expected duration of the study is 24 months. • Part I: Assessment of PEF and FEV1 variability in healthy children and adolescents Duration: 9 months (January - September 2020).

   PEF and FEV1 measurements from 100 participants (3 months each)

   • Part II: Assessment of PEF and FEV1 variability in asthmatic children and adolescents Duration: 15 months (October 2020 - December 2021). PEF and FEV1 measurements from 100 participants (3 months each)

   G. Ethics The study will comply to the regulations and standards of good medical practice. Participants will receive a unique study number and no personal data will be recorded. Measurements will be dispatched via email in encrypted pdf format. Informed written parental consent will be obtained prior to enrollment. Children aged >12 years will also provide a written consent. The study has been approved by the Ethics Committee of the University Hospital of Patras, Greece (decision # 329/02-04-2019).

• Study Type: Interventional
• Enrollment (Anticipated): 200
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Sotirios Fouzas, MD, PhD; Phone Number: (+30) 2610999980; Email: sfouzas@upatras.gr

Study Locations

• Greece
  • Achaia
    • Patras, Achaia, Greece, 26504
      • Recruiting
      • Pediatric Respiratory Unit, University Hospital of Patras
      • Contact: Sotirios Fouzas, MD, PhD; Phone Number: (+30) 2610999980; Email: sfouzas@upatras.gr
      • Principal Investigator: Irini Frima
      • Sub-Investigator: Dimos Gidaris
      • Sub-Investigator: Nikolaos Karantaglis
      • Sub-Investigator: Grigorios Chatziparasidis
      • Sub-Investigator: Olga Lagiou
      • Sub-Investigator: Ilias Theodorakopoulos
      • Sub-Investigator: Panagiotis Plotas

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 6 years to 18 years (Child, Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Cohort A. Healthy children and adolescents

Inclusion Criteria:

• Age 6 - 18 years
• No asthma or asthma medication in the last 2 years
• Normal baseline spirometry
• Smartphone possession (parents or participants) with ability of internet connection (Wi-Fi or mobile data)
• Informed written consent (parents, parents & children >12 years old)

Exclusion Criteria:

• Inability to perform 3 consecutive measurements within the 3-month period of observation
• Inability to perform 6 measurements in total (3.3% of the anticipated 180 measurements) within the 3-month period of observation
• Major respiratory events (e.g., asthma attack, severe respiratory infection, chest trauma) or other health-related events that may hamper the performance of lung function measurements (e.g., surgery, trauma) within the 3-month period of observation.

Cohort B. Children and adolescents with asthma

Inclusion Criteria:

• Age 6 - 18 years
• Doctor-diagnosed asthma under treatment
• Smartphone possession (parents or participants) with ability of internet connection (Wi-Fi or mobile data)
• Informed written consent (parents, parents & children >12 years old)

Exclusion Criteria:

• Inability to perform 3 consecutive measurements within the 3-month period of observation
• Inability to perform 6 measurements in total (3.3% of the anticipated 180 measurements) within the 3-month period of observation
• Major respiratory events (e.g., severe respiratory infection, chest trauma) or other health-related events that may hamper the performance of lung function measurements (e.g., surgery, trauma) within the 3-month period of observation

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Diagnostic
• Allocation: Non-Randomized
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Healthy children and adolescents; Healthy children and adolescents aged 6 to 18 years (N=100) for the assessment of normal PEF and FEV1 variability.	Diagnostic test: Measurements of PEF and FEV1; Measurements of PEF and FEV1 at home, twice a day (07:00 - 09:00 and 19:00 - 21:00) and for a period of 3 months, using portable spirometers connected to smartphone
Experimental: Asthmatic children and adolescents; Children and adolescents aged 6 to 18 years with diagnosed asthma (N=100) for the assessment of PEF and FEV1 variability in asthmatics.	Diagnostic test: Measurements of PEF and FEV1; Measurements of PEF and FEV1 at home, twice a day (07:00 - 09:00 and 19:00 - 21:00) and for a period of 3 months, using portable spirometers connected to smartphone

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
PEF variability	Daily fluctuations in PEF values	3 months
FEV1 variability	Daily fluctuations in FEV1 values	3 months

Collaborators and Investigators

• Sponsor: University of Patras
• Investigators: Study Chair: Sotirios Fouzas, University of Patras

Publications and helpful links

General Publications

• Frima ES, Theodorakopoulos I, Gidaris D, Karantaglis N, Chatziparasidis G, Plotas P, Anthracopoulos M, Fouzas S. Lung Function Variability in Children and Adolescents With and Without Asthma (LUV Study): Protocol for a Prospective, Nonrandomized, Clinical Trial. JMIR Res Protoc. 2020 Aug 7;9(8):e20350. doi: 10.2196/20350.

Study record dates

Study Major Dates

• Study Start (Actual): January 1, 2020
• Primary Completion (Anticipated): January 1, 2022
• Study Completion (Anticipated): January 1, 2023

Study Registration Dates

• First Submitted: November 9, 2019
• First Submitted That Met QC Criteria: November 13, 2019
• First Posted (Actual): November 14, 2019

Study Record Updates

• Last Update Posted (Actual): April 28, 2021
• Last Update Submitted That Met QC Criteria: April 27, 2021
• Last Verified: April 1, 2021

More Information

Terms related to this study

• Keywords: Adolescents; Asthma; Children; Lung function variability
• Additional Relevant MeSH Terms: Respiratory Tract Diseases; Immune System Diseases; Lung Diseases; Hypersensitivity, Immediate; Bronchial Diseases; Lung Diseases, Obstructive; Respiratory Hypersensitivity; Hypersensitivity; Asthma
• Other Study ID Numbers: LUV_2019

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No