Mechanistic Evaluation of Treatments for Acute Antibody-Mediated Rejection of the Kidney Transplant (MOT-AMR)

The best treatment for kidney failure is a kidney transplant, but a transplanted kidney only works for about 10 to 15 years on average. One of the reasons that a transplanted kidney can stop working is that the body develops antibodies against it. Antibodies are proteins produced by the body to fight infections. They play a vital role in dealing with infection but in some transplant patients they can 'fight' the organ, meaning it could stop working. This is called acute antibody-mediated rejection (AAMR). A patient experiencing AAMR can be treated to extend the life of the transplanted kidney, but the chances of the kidney still working 4 years later are reduced. There is currently a clinical trial for UK kidney transplant patients who develop AAMR, called TAR:GET-1. Patients participating in TAR:GET-1 will either receive the standard treatment that is currently given in this situation, or the standard treatment with the addition of rituximab. TAR:GET-1 will answer the question: does adding rituximab to standard treatment lengthen the life of a kidney transplant? A second, sub-study is being proposed of the patients enrolled in TAR:GET-1, that will use the existing blood and biopsy samples already taken during TAR:GET-1 plus an optional extra biopsy of the kidney transplant, to improve our understanding of how the treatments of AAMR work. Patients enrolled in TAR:GET-1 will have had a blood test and biopsy of the transplanted kidney to establish the diagnosis of AAMR. They will also have had further blood tests after treatment, and may also have further biopsies taken if their clinician needs these as part of normal care. Material left over in these samples can be used to analyse how treatment works. In addition, patients will be asked if they agree to an extra biopsy of the transplanted kidney 6 months after the treatment begins. These samples will be analysed at a deeper level than would normally be done, looking at the antibodies and biopsies in detail to answer 2 key questions: 1) Can the unique characteristics noted in an individual patient's antibodies and biopsy predict whether a kidney transplant will be lost as a result of AAMR?; 2) Can we tell treatment is working by looking at the changes in a patient's antibodies and biopsies before and after treatment? The answers to these questions will help us understand AAMR and how its treatments work, and potentially improve our ability to select the right treatment for the right patients.

Study Overview

• Status: Not yet recruiting
• Conditions: Transplant; Complication, Rejection
• Intervention / Treatment: Drug: Rituximab

Detailed Description

Design: Prospective observational study of patients enrolled in TAR:GET-1

Objectives: TAR-GET-1 (starting in 2019) is a multi-centre UK clinical trial that will compare standard of care (SOC) treatment of Acute Antibody-Mediated Rejection of the Kidney Transplant (AAMR) to standard of care plus rituximab (SOCR). This trial presents a rare opportunity to investigate the mechanisms of action of treatments (including rituximab) in AAMR. The rationale for using rituximab is the depletion of CD20-positive precursors to antibody-producing cells. However, rituximab has effects on other B cell subtypes and not all studies have shown antibody level reductions. Furthermore, AAMR is a heterogeneous disease, as defined by variability in mechanistic markers of AAMR in blood (B cell and antibody characteristics) and in transplant biopsies (histological features and gene expression).

The objectives of this study are:

1. To investigate how treatment with rituximab affects these mechanistic markers, by comparing SOC to SOCR
2. To predict treatment benefit early, by analysing the effect of treatment on the mechanistic markers

Methodology: In patients enrolled in TAR:GET-1 (n=170), mechanistic markers will be analysed with established proof of concept (B cell and antibody characteristics, histological and transcriptional markers in the biopsy) at baseline and on follow-up, making use of clinical data, surplus diagnostic material from TAR:GET-1, and material from optional extra blood samples for research taken as part of TAR:GET-1, at 3,6 and 12 months. An optional protocol biopsy at 6 months post treatment will also be offered.

TARGET-1 is assessing DSA MFI and target and B lymphocyte numbers at baseline and 3 months post-treatment. This study will in addition analyse:

1. Serum DSA MFI and target at 6 months; complement-activating ability at baseline, 3 months and 6 months;
2. Biopsy Banff histological scores at baseline and in post-treatment indication biopsies and an optional protocol biopsy;
3. Biopsy AAMR-related transcript signature, assessed using Nanostring nCounter technology, at baseline and in post-treatment biopsies indication biopsies and an optional protocol biopsy;
4. B lymphocyte numbers at 6 months.

Differences pre- and post-treatment will be analysed comparing SOC and SOCR. Mediation analysis models will be used to decompose total effect of treatment effect on graft loss at 4 years (primary end point) into an indirect effect, which measures how much of the effect acts through the intermediate variable(s), and a residual direct effect.

Number of Subjects and Study populations: 170 subjects enrolled in TAR:GET-1

Outcome measures: same as primary outcome of TAR:GET-1 (graft loss at 4 years after initiation of treatment)

Inclusion Criteria: All patients enrolled in TAR:GET-1 ; Signed informed consent prior to any study specific procedures.

Exclusion Criteria: None

Duration of study: 8 years (duration is dictated by the need for primary outcome of TAR-GET-1 to be reached)

Statistical Methods: Data from this research project will be merged and linked with data from the TAR:GET-1 clinical trial by the statistician team.

Descriptive data: This will include description of all the biological characteristics analysed in 2 populations (rituximab + SOC and SOC) at baseline and at all follow-up time points. Summary statistics (mean, median, SD, range for continuous variables, and frequency tables for categorical variables) will be reported broken down by treatment, with exploratory figures (box-and-whisker plots for continuous, and stacked bar-charts for categorical).

Outcome analysis: Appropriate measures of differences between treatment groups will be estimated with point estimates, 95% confidence intervals, and p-values. Depending on the type of variable (continuous, categorical, time-to-event), regression techniques (linear, logistic, cox) and transformation of the endpoints will be considered.

Differences in characteristics comparing pre- to post-treatment samples at sequential time points will be analysed comparing the 2 groups of patients (SOCR versus SOC) using r mixed effect models repeat measurement techniques.

Mediation analysis, a special case of regression analysis, will be used to document that the treatment effects a change in the biomarker. Mediation analysis models will be used to decompose total effect of treatment effect on graft loss into an indirect effect, which measures how much of the effect acts through the intermediate variable(s), and a residual direct effect. All the mediation analyses will be based on the Intention-to-Treat principle. The same form of outcome models will be fitted as for the efficacy analyses, including the mediator as a covariate. The mediation package within R will be used which was developed specifically to make valid causal inference in explanatory analyses of the mechanisms of treatment-induced change in clinical outcomes in randomised clinical trials. All estimates of treatment differences will be summarised using effect size estimates and 95% confidence intervals (Dunn 2013). Collaborator for mediation analysis: Prof Ian White, Professor of Statistical Methods for Medicine, UCL.

• Study Type: Observational
• Enrollment (Anticipated): 170

Contacts and Locations

• Study Contact: Name: Candice Roufosse, MD PhD; Phone Number: 02033138240; Email: c.roufosse@imperial.ac.uk

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 3 years and older (Child, Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

All patients enrolled in TAR:GET-1

Description

Inclusion Criteria:

All patients enrolled in TAR:GET-1 Signed informed consent prior to any study specific procedures.

Exclusion Criteria:

None

Study Plan

How is the study designed?

Design Details

• Observational Models: Cohort
• Time Perspectives: Prospective

Number of groups / cohorts

2

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
Standard of care (SOC); See NCT03994783
Rituximab + SOC (SOCR); See NCT03994783	Drug: Rituximab; See NCT03994783

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Allograft Survival as assessed by statistical model	Statistical model measuring allograft survival as defined as duration from the date of randomisation to the date of eGFR ≤15 mL/min/1.72 m2, or the date of renal replacement therapy (date of starting dialysis dependency, transplantation etc), whichever occurs first.	4 years

Collaborators and Investigators

• Sponsor: Imperial College London
• Collaborators: University of Cambridge; Viapath LLP
• Investigators: Principal Investigator: Candice Roufosse, MD PhD, Imperial College London

Study record dates

Study Major Dates

• Study Start (Anticipated): September 1, 2020
• Primary Completion (Anticipated): July 1, 2027
• Study Completion (Anticipated): August 31, 2028

Study Registration Dates

• First Submitted: July 29, 2020
• First Submitted That Met QC Criteria: July 29, 2020
• First Posted (Actual): August 3, 2020

Study Record Updates

• Last Update Posted (Actual): August 3, 2020
• Last Update Submitted That Met QC Criteria: July 29, 2020
• Last Verified: July 1, 2020

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Physiological Effects of Drugs; Antirheumatic Agents; Antineoplastic Agents; Immunologic Factors; Antineoplastic Agents, Immunological; Rituximab
• Other Study ID Numbers: 19HH5204

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: No

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No