- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT04961255
Effects of Post-COVID-19 on Quadriceps Femoral Muscle-tendon Unit
Effects of Post-COVID-19 on Neural Activation and Architecture of the Quadriceps Femoral Muscle-tendon Unit and Its Correlation With Inflammatory Markers
Study Overview
Status
Conditions
Detailed Description
Study Type
Enrollment (Estimated)
Contacts and Locations
Study Contact
- Name: Rita CM Durigan, PhD
- Phone Number: +55 61 3107-8450
- Email: marqueti@gmail.com
Study Contact Backup
- Name: João LQ Durigan, PhD
- Phone Number: +55 61 3107-8450
- Email: joaodurigan@gmail.com
Study Locations
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Distrito Federal
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Brasília, Distrito Federal, Brazil, 72220-900
- Recruiting
- University of Brasilia
-
Contact:
- João LQ Durigan, PhD
- Phone Number: +55 61 3107-8450
- Email: joaodurigan@gmail.com
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Contact:
- isabella S Almeida
- Phone Number: +55 613356-9148
- Email: isabellafisioucb@gmail.com
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-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Sampling Method
Study Population
Description
Inclusion Criteria:
Control group:
- The control group will include healthy participants, aged 18 and 80 years, who had not tested positive for COVID-19.
COVID-19 positive participants who had moderate symptoms group:
- Female and male participants, aged between 18 and 80 years, who had Molecular test (RT-PCR) or serological test, which assesses whether the samples are reactive to immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies, positive for COVID-19;
- Presence of some of the following symptoms, without the need for hospitalization: dry cough, runny nose, sore throat, diffuse body pain, persistent hyperthermia, no hypoxemia.
COVID-19 positive participants who had severe symptoms group:
- Female and male participants, aged between 18 and 80 years, who had Molecular test (RT-PCR) or serological test (which assesses whether the samples are reactive to IgM and IgG antibodies) positive for COVID-19;
- Presence of hypoxemia (peripheral oxygen saturation - SPO2 ≤ 93%) requiring hospitalization with or without intubation, in addition to the symptoms described for the COVID-19 positive participants who had moderate symptoms group.
Exclusion Criteria:
- Body Mass Index (BMI) ≥ 35 kg/m2;
- Pregnancy;
- Pain, swelling, dermal damage, deformity, or amputation in the regions to be examined;
- Diagnosis of ankylosing spondylitis, rheumatoid arthritis, severe heart disease, advanced chronic obstructive pulmonary disease, cognitive impairment, chemical dependency, psychiatric illness, or behavioral that makes it difficult to cooperate with the procedures.
Study Plan
How is the study designed?
Design Details
Cohorts and Interventions
Group / Cohort |
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Control group
Healthy individuals will be evaluated only once throughout the study.
Inflammatory markers, the isometric maximum voluntary force of knee extensor torque, muscle fatigue, neuromuscular adaptations, muscle architecture, quadriceps tendon properties, peripheral oxygen extraction, and body composition will be evaluated.
|
COVID-19 positive participants who had moderate symptoms group
These participants will be evaluated 4 times throughout the study. Baseline 1: This will be performed between 21 to 30 days after the onset of symptoms of COVID-19 infection. Assessment 2: This will be performed between 31 to 90 days after the onset of symptoms of COVID-19 infection. Assessment 3: This will be carried out between 91 and 180 days after the onset of symptoms of COVID-19 infection. Assessment 4: This will be performed between 181 to 360 days after the onset of symptoms of COVID-19 infection. Inflammatory markers, the isometric maximum voluntary force of knee extensor torque, muscle fatigue, neuromuscular adaptations, muscle architecture, quadriceps tendon properties, peripheral oxygen extraction, and body composition will be evaluated in each assessment. |
COVID-19 positive participants who had severe symptoms group
These participants will be evaluated 4 times throughout the study. Baseline 1: This will be performed between 21 to 30 days after the onset of symptoms of COVID-19 infection. Assessment 2: This will be performed between 31 to 90 days after the onset of symptoms of COVID-19 infection. Assessment 3: This will be carried out between 91 and 180 days after the onset of symptoms of COVID-19 infection. Assessment 4: This will be performed between 181 to 360 days after the onset of symptoms of COVID-19 infection. Inflammatory markers, the isometric maximum voluntary force of knee extensor torque, muscle fatigue, neuromuscular adaptations, muscle architecture, quadriceps tendon properties, peripheral oxygen extraction, and body composition will be evaluated in each assessment. |
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Blood biomarkers
Time Frame: Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
|
Blood biomarkers including metalloproteinase 9, interleukin-6, interleukin-8 and interleukin-10, interleukin 1 beta, tumor necrosis factor alpha type, nitric oxide, as well as creatine kinase will be collected in blood samples by venipuncture in the vein brachial, in heparinized tubes with separating gel using the vacutainer system with ethylenediaminetetraacetic acid (EDTA).
The samples will be stored and later analyzed using appropriate techniques.
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Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
|
Sit to stand test
Time Frame: Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
|
To carry out the test will be used a stopwatch and a chair with the back against a wall, seat height 43 cm from the floor and without armrest.
The test consists of measuring how many times the participant can get up and sit down from a chair (counting as a movement) in a period of 30 seconds.
|
Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
|
Electrodiagnostic stimulus test
Time Frame: Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
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The quadriceps femoris, gastrocnemius, soleus and tibialis anterior muscles will be evaluated.
At the muscle motor point, the values of rheobase, chronaxia and accommodation will be investigated.
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Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
|
Body composition
Time Frame: Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
|
Body composition will be estimated using dual x-ray absorptiometry.
Fat mass and fat-free mass will be expressed in absolute values.
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Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
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Muscle force
Time Frame: Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
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Muscle force will be assessed by muscle force changes, based on the torque generated during maximum voluntary contraction.
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Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
|
Peripheral oxygen extraction
Time Frame: Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
|
The assessment of peripheral oxygen extraction will be performed both in rest and during voluntary contraction.
The evaluation will be carried out by means of near-infrared spectroscopy (NIRS or near infrared spectroscopy).
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Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
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Surface electromyographic activity
Time Frame: Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
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Electromyographic activity of each superficial component of the quadriceps muscle both in rest and during voluntary contraction.
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Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
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Muscle architecture
Time Frame: Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
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Muscle architecture analyzes will be performed using ultrasound at rest and during voluntary contraction.
The muscle volume of the knee extensors, muscle thickness, pennation angle, fascicle length and muscle echogenicity will be evaluated.
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Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
|
Properties of the patellar tendon
Time Frame: Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
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The morphological, material and mechanical properties will be evaluated based from the elongation of the patellar tendon during maximum voluntary contraction.
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Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
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Muscle fatigue
Time Frame: Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
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Quadriceps muscle fatigue will be assessed in each leg after consecutive maximal isometric voluntary contractions of the quadriceps muscle.
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Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
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Assessment of perceived exertion during the fatigue protocol
Time Frame: Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
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Assessment of perceived exertion will be analyzed before, during, and after the performance of maximum voluntary isometric contractions performed to analyze muscle fatigue using the BORG scale of perceived exertion.
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Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
|
Fatigue severity analysis
Time Frame: Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
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Fatigue severity analysis will be assessed using the fatigue severity scale.
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Change from baseline 1 (days 21-30) to assessments 2 (days 31-90), 3 (days 91-180), and 4 (days 181 to 360) for groups composed of COVID-19 positive participants. The Control group will be assessed only at baseline.
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Collaborators and Investigators
Sponsor
Investigators
- Principal Investigator: João LQ Durigan, PhD, University of Brasilia
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Estimated)
Study Completion (Estimated)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Estimated)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Keywords
Additional Relevant MeSH Terms
Other Study ID Numbers
- CAAE: 45043821.0.0000.8093
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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