- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT06097130
Batch Fermentation and Microbiome Analysis
October 23, 2023 updated by: Société des Produits Nestlé (SPN)
Evaluation of the Effect of Different Nutrients on the Gut Microbiota Composition and Metabolic Activity by Using in Vitro Batch Fermentation and Cellular Models
The correlation between diet and microbiota as well as the effect of microbiota on human health is well established.
Studies have shown that people following specific diets have different microbiome profile compared to those following traditional or ordinary diets.
In order to screen several food ingredients for their effects on human microbiota composition and metabolic activities, in vitro studies are proposed.
Nutrients are added in-vitro in fecal sample, eliminating the need for participants to consume any nutritional product.
The batch fermentation in vitro system simulates human colonic microbiota from fecal samples, enabling the complex mixture of microorganisms.
This system could serve as a simple model to simulate the diversity as well as the metabolism of human colonic microbiota.
We will evaluate the effect on nutrient/s on the age specific microbiome via an in vitro fermentation approach for the 0 to 60 year-old period.
Study Overview
Status
Not yet recruiting
Conditions
Study Type
Observational
Enrollment (Estimated)
100
Contacts and Locations
This section provides the contact details for those conducting the study, and information on where this study is being conducted.
Study Contact
- Name: Pamela Sun, MBBChir MPhil (Cantab)
- Phone Number: +41 21 785 81 22
- Email: pamela.sun@rd.nestle.com
Study Contact Backup
- Name: Sylviane Oguey-Araymon
- Phone Number: +41217858279
- Email: sylviane.oguey-araymon@rdls.nestle.com
Study Locations
-
-
Vaud
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Lausanne, Vaud, Switzerland, 1000
- Nestle Clinical Innovation Lab
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Participation Criteria
Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.
Eligibility Criteria
Ages Eligible for Study
- Child
- Adult
Accepts Healthy Volunteers
Yes
Sampling Method
Probability Sample
Study Population
Healthy population
Description
Inclusion Criteria:
- Healthy participant aged from 0 to 60 (based on medical history, physical examination and review of childhood growth chart)
- Written informed consent has been obtained from the parent(s)/legally acceptable representative (LAR) of toddlers and teenagers
- Written informed consent has been obtained for participants aged from 14 years old and above
- Child's parent(s)/legal representative or participant is willing and able to fulfill the requirements of the study protocol.
- Ability to collect their or their infant/child's fecal sample.
Exclusion Criteria:
- The parent(s)/legally acceptable representative (LAR) is not able to provide evidence of parental authority or legal representation.
- Participant following a particular regimen of any type such as vegan, vegetarian, ketogenic, paleo diet,
- Chronic or recurrent diarrhea with spontaneous bowel movements more often than 2 times daily,
- Antibacterial/antifungal therapy during the 3 months prior to study enrolment,
- Medications or supplements that are known to alter gut function or microbiota (i.e. acid antisecretory drugs, pre-/probiotics supplements, laxatives) during the 4 weeks prior to study enrolment,
- Prior gastrointestinal surgery (apart from appendectomy, cholecystectomy or herniotomy),
- Alcohol intake higher than 2 servings per day. A serving is 0.4 dl of strong alcohols, 1 dl of red or white wine, or 3 dl of beer,
- Artificially sweetened beverage intake higher than 1000 ml/ per day,
- Current or history of gastrointestinal diseases like (Celiac Disease, Crohn's Disease, Ulcerative colitis, Irritable Bowel Syndrome, infantile colic, recurrent abdominal pain, functional constipation, ulcers, infections (based on anamnesis),
- Participants participating in another interventional study,
- Participants/parents having a hierarchical or family link with the research team members.
Study Plan
This section provides details of the study plan, including how the study is designed and what the study is measuring.
How is the study designed?
Design Details
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Change in microbiota composition
Time Frame: Through study completion, an average of 1 year
|
Measured by 16s rRNA sequencing or next Generation Sequencing (NGS) or by qPCR
|
Through study completion, an average of 1 year
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Change in Short Chain Fatty Acids (SCFA)
Time Frame: Through study completion, an average of 1 year
|
Measured using Gas Chromatography (GC)
|
Through study completion, an average of 1 year
|
Change in metabolic profile
Time Frame: Through study completion, an average of 1 year
|
Measured using Gas Chromatography (GC) or Gas chromatography-mass spectrometry (GC-MS)
|
Through study completion, an average of 1 year
|
Protein expression of epithelial cells
Time Frame: Through study completion, an average of 1 year
|
Measured by western blot
|
Through study completion, an average of 1 year
|
Collaborators and Investigators
This is where you will find people and organizations involved with this study.
Study record dates
These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.
Study Major Dates
Study Start (Estimated)
January 15, 2024
Primary Completion (Estimated)
January 31, 2025
Study Completion (Estimated)
January 31, 2025
Study Registration Dates
First Submitted
March 16, 2023
First Submitted That Met QC Criteria
October 23, 2023
First Posted (Actual)
October 24, 2023
Study Record Updates
Last Update Posted (Actual)
October 24, 2023
Last Update Submitted That Met QC Criteria
October 23, 2023
Last Verified
October 1, 2023
More Information
Terms related to this study
Keywords
Other Study ID Numbers
- 1920NR
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
UNDECIDED
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
No
Studies a U.S. FDA-regulated device product
No
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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