Exploration of Gender Differences in Liver Aging

The liver is an important metabolic organ in the body. It participates in central metabolic activities to maintain systemic homeostasis. It is also one of the most vulnerable organs in the process of individual aging. During aging, the volume, blood flow, metabolic capacity and regenerative capacity of the liver decrease. Liver function changes with age can lead to age-related systemic susceptibility diseases.

Therefore, understanding the changes of metabolism and immune function in the process of liver aging and studying the regulation mechanism of liver aging will provide an important reference for understanding the causes of increased susceptibility to liver-related diseases in the elderly population.On the other hand, gender factors also affect organ function, leading to differences in human aging and disease outcomes.At present, the omics research on liver aging is mainly based on tissue-level transcription, protein or metabolomics, and most of the conclusions are focused on hepatocytes. However, for other cell types and microenvironment interactions, people know little about the aging changes at the cellular levelBased on the current research status, this study aims to explore the gender differences in the process of liver aging, using emerging technologies such as the combination of single-cell multi-omics and spatial localization, to reveal the factors driving liver aging from a gender perspective, and to promote the development of accurate interventions for aging and aging-related diseases.

Study Overview

• Status: Recruiting
• Conditions: Healthy Population

• Study Type: Observational
• Enrollment (Estimated): 18

Contacts and Locations

• Study Contact: Name: daren Liu, MD, PhD; Phone Number: 15968179036; Email: darenliu@zju.edu.cn

Study Locations

• China
  • Zhejiang
    • Hangzhou, Zhejiang, China, No.88 Jiefang Road
      • Recruiting
      • 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China
      • Contact: Liu Daren, MD, PhD; Phone Number: +86-15968179036; Email: darenliu@zju.edu.cn

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult; Older Adult
• Accepts Healthy Volunteers: Yes

• Sampling Method: Non-Probability Sample

Study Population

In this study, the normal liver sampling criteria are as follows : 1.Preoperative blood biochemical liver function indicators are in the normal reference range.

2.Liver imaging is normal. The liver is normal in size, regular in shape, smooth in capsule, uniform in parenchymal echo, and intrahepatic vascular network.

For patients undergoing laparoscopic cholecystectomy, the liver tissue of the IV segment of the liver ( about 1mm3 ) was separated from the gallbladder wall specimen.

Description

Inclusion Criteria:

• Liver function indicators are in the normal reference range.
• Liver imaging examination showed normal.

Exclusion Criteria:

• Patients with previous liver-related diseases.
• Subjects with important organ dysfunction / failure or other serious diseases ( myocardial infarction, malignant tumor, severe neurological or psychiatric history, severe infection, coagulation dysfunction, etc. )
• Moderate malnutrition ( BMI < 17kg / m2 ) and severe anemia ( Hb < 60g / L ).
• Subjects and their families who disagreed with the study protocol.

Study Plan

How is the study designed?

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Molecular and cellular characteristics during liver aging	Draw the map information of liver aging single cell precision, and clarify whether the effect of aging on liver cell types has gender differences.	2027.08

Collaborators and Investigators

• Sponsor: Second Affiliated Hospital, School of Medicine, Zhejiang University
• Investigators: Study Director: daren Liu, MD, PhD, Zhejiang University

Study record dates

Study Major Dates

• Study Start (Actual): September 27, 2024
• Primary Completion (Estimated): September 15, 2027
• Study Completion (Estimated): September 20, 2027

Study Registration Dates

• First Submitted: September 8, 2024
• First Submitted That Met QC Criteria: September 8, 2024
• First Posted (Actual): September 19, 2024

Study Record Updates

• Last Update Posted (Actual): October 8, 2024
• Last Update Submitted That Met QC Criteria: October 6, 2024
• Last Verified: September 1, 2024

More Information

Terms related to this study

• Keywords: sex differences; hepatocyte; liver function
• Other Study ID Numbers: 2024-0815

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No