Resistance Exercise Volume and Hypertrophic Response Variability in Elderly

Effects of Resistance Exercise Volume on the Variability of the Hypertrophic Response in Elderly

The study aimed to investigate the effects of resistance training volume manipulation on whole muscle hypertrophy among non-responders and responders to a low resistance training dose (single-set).

Study Overview

• Status: Completed
• Conditions: Aging; Muscle Hypertrophy
• Intervention / Treatment: Other: Lower body resistance training

Detailed Description

The magnitude of muscle hypertrophy in response to resistance training (RT) is highly variable between individuals (response heterogeneity). Manipulations in RT variables may modulate RT-related response heterogeneity; yet, this remains to be determined.

• Study Type: Interventional
• Enrollment (Actual): 102
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Brazil
  • SP
    • Sao Paulo, SP, Brazil, 05508-030
      • University of Sao Paulo

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult; Older Adult
• Accepts Healthy Volunteers: No

Description

Inclusion Criteria:

• 60 years and older
• not engaged in regular resistance and/or aerobic training for at least six months before the intervention

Exclusion Criteria:

• diagnosed type I diabetes mellitus
• arrhythmia
• noncontrolled hypertension
• any major orthopedic musculoskeletal condition
• use of any dietary supplement or drug with potential anabolic effects

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Other
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Single

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Single set; One set of unilateral knee-extension exercise of 8-15 RM with a 60-90 s interval.	Other: Lower body resistance training; The RT protocol was performed using a unilateral knee-extension machine. To reduce potential sources of bias to exercise order, subjects alternated the starting leg for each training session.
Experimental: Four sets; Four sets of unilateral knee-extension exercise of 8-15 RM with a 60-90 s interval between sets.	Other: Lower body resistance training; The RT protocol was performed using a unilateral knee-extension machine. To reduce potential sources of bias to exercise order, subjects alternated the starting leg for each training session.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Quadriceps muscle cross-sectional area	Quadriceps muscle CSA was obtained through magnetic resonance imaging.	10 weeks

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Maximum dynamic strength	Unilateral quadriceps 1-RM of both legs were assessed on a knee-extension machine	10 weeks

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Dietary assessment	Dietary assemment using the USDA Automated Multiple-Pass Method	Before intervention
Mass spectrometry	Mass spectrometry metabolomics	10 weeks
Skeletal muscle RNA-sequencing	RNA sequencing using an Illumina NovaSeq Platform with a standard paired-end 150 bp (PE150) dual indexing protocol	10 weeks
Muscle protein measures	Upstream binding factor (UBF) muscle protein was quantified by Simple Western capillary immunoassay.	10 weeks
Muscle protein measures	v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (c-Myc) muscle protein was quantified by Simple Western capillary immunoassay.	10 weeks
Muscle protein measures	Frizzled class receptor 1 (Frizzled-1) muscle protein was quantified by Simple Western capillary immunoassay.	10 weeks
Muscle protein measures	ribosomal protein L3 (rpL3) muscle protein was quantified by Simple Western capillary immunoassay.	10 weeks

Collaborators and Investigators

• Sponsor: University of Sao Paulo General Hospital
• Collaborators: University of Sao Paulo

Study record dates

Study Major Dates

• Study Start (Actual): April 1, 2018
• Primary Completion (Actual): December 20, 2018
• Study Completion (Actual): December 20, 2018

Study Registration Dates

• First Submitted: December 1, 2024
• First Submitted That Met QC Criteria: December 1, 2024
• First Posted (Actual): December 5, 2024

Study Record Updates

• Last Update Posted (Estimated): December 11, 2024
• Last Update Submitted That Met QC Criteria: December 5, 2024
• Last Verified: November 1, 2024

More Information

Terms related to this study

• Keywords: muscle strength; muscle mass; aging; resistance exercise; sets
• Additional Relevant MeSH Terms: Pathological Conditions, Anatomical; Hypertrophy
• Other Study ID Numbers: 62590416.0.0000.5391

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No