Dietary supplementation with a superoxide dismutase-melon concentrate reduces stress, physical and mental fatigue in healthy people: a randomised, double-blind, placebo-controlled trial

Julie Carillon, Claire Notin, Karine Schmitt, Guy Simoneau, Dominique Lacan, Julie Carillon, Claire Notin, Karine Schmitt, Guy Simoneau, Dominique Lacan

Abstract

Background: We aimed to investigate effects of superoxide dismutase (SOD)-melon concentrate supplementation on psychological stress, physical and mental fatigue in healthy people.

Methods: A randomized, double-blind, placebo-controlled trial was performed on 61 people divided in two groups: active supplement (n = 32) and placebo (n = 29) for 12 weeks. Volunteers were given one small hard capsule per day. One capsule contained 10 mg of SOD-melon concentrate (140 U of SOD) and starch for the active supplement and starch only for the placebo. Stress and fatigue were evaluated using four psychometric scales: PSS-14; SF-36; Stroop tests and Prevost scale.

Results: The supplementation with SOD-melon concentrate significantly decreased perceived stress, compared to placebo. Moreover, quality of life was improved and physical and mental fatigue were reduced with SOD-melon concentrate supplementation.

Conclusion: SOD-melon concentrate supplementation appears to be an effective and natural way to reduce stress and fatigue.

Trial registration: trial approved by the ethical committee of Poitiers (France), and the ClinicalTrials.gov Identifier is NCT01767922.

Figures

Figure 1
Figure 1
Scores of PSS-14 scale (Mean ± SEM) before, between and after supplementation. Transverse comparison between groups are not significantly different (NS) for D0, but significantly different (* p < 0.05) for D28 and D84.
Figure 2
Figure 2
Scores of SF 36 scale (Mean ± SEM) before, between and after supplementation. Transverse comparison between groups are not significantly different (NS) for D0 and D28, but significantly different (* p < 0.05) for D84.
Figure 3
Figure 3
Scores of Stroop scale (Mean ± SEM) before, between and after supplementation. Transverse comparison between groups are not significantly different (NS) for D0, but significantly different (* p < 0.05; *** p < 0.001) for D28 and D84.
Figure 4
Figure 4
Scores of Prevost scale (Mean ± SEM) before, between and after supplementation. Transverse comparison between groups are not significantly different (NS) for D0 and D28, but significantly different (* p < 0.05) for D84.

References

    1. Casado A., de Lucas García N., López-Fernández M.E., Sánchez Rodríguez-Manzaneque A., Jiménez Fraile J.A. Antioxidant enzymes, occupational stress and burnout in workers of a prehospitalary emergency service. Eur. J. Emerg. Med. 2005;12:111–115. doi: 10.1097/00063110-200506000-00003.
    1. Casado A., Castellanos A., Lopez-Fernandez M.E., Ruiz R., Garcia Aroca C., Noriega F. Relationship between oxidative and occupational stress and aging in nurses of an intensive care unit. Age. 2008;30:229–236. doi: 10.1007/s11357-008-9052-5.
    1. Lucca G., Comim C.M., Valvassori S.S., Réus G.Z., Vuolo F., Petronilho F., Dal-Pizzol F., Gavioli E.C., Quevedo J. Effects of chronic mild stress on the oxidative parameters in the rat brain. Neurochem. Int. 2009;54:358–362. doi: 10.1016/j.neuint.2009.01.001.
    1. Maes M., Galecki P., Chang Y.S., Berk M. A review on the oxidative an nitrosative stress (O & NS) pathways in major depression and their possible contribution to the (neuro)degenerative processes in that illness. Progr. Neuro-Psychopharmacol. Biol. Psych. 2011;35:676–692. doi: 10.1016/j.pnpbp.2010.05.004.
    1. Maes M., Fisar Z., Medina M., Scapagnini G., Nowak G., Berk M. New drug targets in depression: Inflammatory, cell-mediated immune, oxidative and nitrosative stress, mitochondrial, antioxidant, and neuroprogressive pathways. And new drug candidates-Nrf2 activators and GSK-3 inhibitors. Inflammopharmacology. 2012;20:127–150. doi: 10.1007/s10787-011-0111-7.
    1. Shichiri M., Harada N., Ishida N., Komaba L.K., Iwaki S., Hagihara Y., Niki E., Yoshida Y. Oxidative stress is involved in fatigue induced by overnight deskwork as assessed by increase in plasma tocopherylhydroqinone and hydroxycholesterol. Biol. Psychol. 2013;94:527–533. doi: 10.1016/j.biopsycho.2013.10.002.
    1. Kennedy G., Spence V.A., McLaren M., Hill A., Underwood C., Belch J.J.F. Oxidative stress levels are raised in chronic fatigue syndrome and are associated with clinical symptoms. Free Radic. Biol. Med. 2005;39:584–589. doi: 10.1016/j.freeradbiomed.2005.04.020.
    1. Morris G., Maes M. Myalgic encephalomyelitis/chronic fatigue syndrome and encephalomyelitis disseminate/multiple sclerosis show remarkable levels of similarity in phenomenology and neuroimmune characteristics. BMC Med. 2013;11:205. doi: 10.1186/1741-7015-11-205.
    1. Tagliari B., Scherer E.B., Machado F.R., Ferreira A.G.K., Dalmaz C., Wyse A.T.S. Antioxidants prevent memory deficits provoked by chronic variable stress in rats. Neurochem. Res. 2011;36:2373–2380. doi: 10.1007/s11064-011-0563-6.
    1. Gupta A., Vij G., Sharma S., Tirkey N., Rishi P., Chopra K. Curcumin, a polyphenolic antioxidant, attenuates chronic fatigue syndrome in murine water immersion stress model. Immunobiology. 2009;214:33–39. doi: 10.1016/j.imbio.2008.04.003.
    1. Yagi A., Fujimoto K., Michihiro K., Goh B., Tsi D., Nagai H. The effect of lutein supplementation on visual fatigue: A psychophysiological analysis. Appl. Ergonom. 2009;40:1047–1054. doi: 10.1016/j.apergo.2009.04.013.
    1. Nakano M., Yamamoto T., Okamura H., Tsuda A., Kowatari Y. Effects of oral supplementation with Pyrroloquinoline Quinone on stress, fatigue, and sleep. Funct. Foods Health Dis. 2012;2:307–324.
    1. Milesi M.A., Lacan D., Brosse H., Desor D., Notin C. Effect of an oral supplementation with a proprietary melon juice concentrate (Extramel®) on stress and fatigue in healthy people: A pilot, double-blind, placebo-controlled clinical trial. Nutr. J. 2009;8:40. doi: 10.1186/1475-2891-8-40.
    1. Ginoux J.P., Dreyer A., Roch P., Baccou J.C., Lacan D. Cucumis Melo Protein Extract with Antioxidant Activity and Process for Preparing it, Cosmetic or Pharmaceutical Composition or Food Composition Containing Such An Extract. 5,616,323. U.S. Patent. 1997 Apr 1;
    1. Zhou J.Y., Prognon P. Raw material enzymatic activity determination: A specific case for validation and comparison of analytical methods-the example of superoxide dismutase (SOD) J. Pharm. Biomed. Anal. 2006;40:1143–1148. doi: 10.1016/j.jpba.2005.09.022.
    1. Cohen S., Kamarck T., Mermelstein R. A global measure of perceived stress. J. Health Soc. Behav. 1983;24:386–396.
    1. Wade J.E., Sherbourne C.D. The MOS-36 item short-form health survey. Med. Care. 1992;30:473–483. doi: 10.1097/00005650-199206000-00002.
    1. Stroop J.R. Studies of interferences in serial verbal reactions. J. Exp. Psychol. 1935;18:643–662. doi: 10.1037/h0054651.
    1. Prévost P. Le surentraînement. Sport Santé Prépar. Phys. 2002;4:1–3.
    1. Hamilton M. The assessment of anxiety states by rating. Br. J. Med. Psychol. 1959;32:50–55. doi: 10.1111/j.2044-8341.1959.tb00467.x.
    1. Cohen S., Kessler R.C., Gordon L.U. Measuring Stress: A Guide for Health and Social Scientists. Oxford University Press; New York, NY, USA: 1997.
    1. Coleman C.A., Friedman A.G., Burright R.G. The relationship of daily stress and health-related behaviors to adolescents’ cholesterol levels. Adolescence. 1998;130:447–460.
    1. Carter-Snell C., Hegadoren K. Stress disorders and gender; implications for theory and research. Can. J. Nurs. Res. 2003;35:34–55.
    1. Houghton C.A., Steelsc E.L., Fassetta R.G., Coombesa J.S. Effects of a gliadin-combined plant superoxide dismutase extract on self-perceived fatigue in women aged 50–65 years. Phytomedicine. 2011;18:521–526. doi: 10.1016/j.phymed.2010.09.006.
    1. Carillon J., Rouanet J.M., Cristol J.P., Brion R. Superoxide dismutase administration, a potential therapy against oxidative stress related diseases: Several routes of supplementation and proposal of an original mechanism of action. Pharm. Res. 2013;30:2718–2728. doi: 10.1007/s11095-013-1113-5.
    1. Carillon J., Romain C., Bardy G., Fouret G., Feillet-Coudray C., Gaillet S., Lacan D., Cristol J.P., Rouanet J.M. Cafeteria diet induces obesity and insulin resistance associated with oxidative stress but not with inflammation. Improvement by dietary supplementation of a melon superoxide dismutase. Free Radic. Biol. Med. 2013;65:254–261. doi: 10.1016/j.freeradbiomed.2013.06.022.
    1. Carillon J., Knabe L., Montalban A., Stevant M., Keophiphath M., Lacan D., Cristol J.P., Rouanet J.M. Curative diet supplementation with a melon superoxide dismutase reduces adipose tissue in obese hamsters by improving insulin sensitivity. Mol. Nutr. Food Res. 2014;58:842–850. doi: 10.1002/mnfr.201300466.
    1. Carillon J., Fouret G., Feillet-Coudray C., Lacan D., Cristol J.P., Rouanet J.M. Short-term assessment of toxicological aspects, oxidative and inflammatory response to dietary melon superoxide dismutase in rats. Food Chem. Toxicol. 2013;55:323–328. doi: 10.1016/j.fct.2013.01.021.
    1. Carillon J., Rugale C., Rouanet J.M., Cristol J.P., Lacan D., Jover B. Endogenous antioxidant defense induction by melon superoxide dismutase reduces cardiac hypertrophy in spontaneously hypertensive rats. Int. J. Food Sci. Nutr. 2014 doi: 10.3109/09637486.2014.893286.
    1. Guidance for Industry on Estimating the Maximum Safe Starting Dose in Initial Clinical Trials for Therapeutics in Adult Healthy Volunteers. [(accessed on 22 July 2005)]; Available online: .

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