Serum levels of cartilage oligomeric matrix protein (COMP) increase temporarily after physical exercise in patients with knee osteoarthritis

Maria L E Andersson, Carina A Thorstensson, Ewa M Roos, Ingemar F Petersson, Dick Heinegård, Tore Saxne, Maria L E Andersson, Carina A Thorstensson, Ewa M Roos, Ingemar F Petersson, Dick Heinegård, Tore Saxne

Abstract

Background: COMP (Cartilage oligomeric matrix protein) is a matrix protein, which is currently studied as a potential serum marker for cartilage processes in osteoarthritis (OA). The influence of physical exercise on serum COMP is not fully elucidated. The objective of the present study was to monitor serum levels of COMP during a randomised controlled trial of physical exercise vs. standardised rest in individuals with symptomatic and radiographic knee OA.

Methods: Blood samples were collected from 58 individuals at predefined time points before and after exercise or rest, one training group and one control group. The physical exercise consisted of a one-hour supervised session twice a week and daily home exercises. In a second supplementary study 7 individuals were subjected to the same exercise program and sampling of blood was performed at fixed intervals before, immediately after, 30 and 60 minutes after the exercise session and then with 60 minutes interval for another five hours after exercise to monitor the short-term changes of serum COMP. COMP was quantified with a sandwich-ELISA (AnaMar Medical, Lund, Sweden).

Results: Before exercise or rest no significant differences in COMP levels were seen between the groups. After 60 minutes exercise serum COMP levels increased (p < 0.001). After 60 minutes of rest the serum levels decreased (p = 0.003). Median serum COMP values in samples obtained prior to exercise or rest at baseline and after 24 weeks did not change between start and end of the study. In the second study serum COMP was increased immediately after exercise (p = 0.018) and had decreased to baseline levels after 30 minutes.

Conclusion: Serum COMP levels increased during exercise in individuals with knee OA, whereas levels decreased during rest. The increased serum COMP levels were normalized 30 minutes after exercise session, therefore we suggest that samples of blood for analysis of serum COMP should be drawn after at least 30 minutes rest in a seated position. No increase was seen after a six-week exercise program indicating that any effect of individualized supervised exercise on cartilage turnover is transient.

Figures

Figure 1
Figure 1
Study design. Blood-sampling procedures during the study from study baseline at -3 weeks to the end of the study at 24 weeks. Blood samples were obtained before and after 60 minutes exercise/rest at each occasion. The arrows indicates blood-sampling occasions. Two blood-samples at each occasions, with an hour apart.
Figure 2
Figure 2
Serum COMP at start of study. Serum concentration of COMP at week -3, before intervention, in study 1. Each line represents an individual patient. Horizontal bars show median values for each group.
Figure 3
Figure 3
Serum COMP during intervention. Serum concentrations of COMP at weeks 0 and 6 in the exercise group in study 1. Each line represents an individual patient. Horizontal bars show median values for each sampling occasion.
Figure 4
Figure 4
Serum COMP after standardised exercise in seven patients recruited from the exercise group in study 1. Serum COMP concentrations after a one-hour supervised exercise session in 7 patients, study 2. Each line represents an individual patient. The inserted figure shows serum COMP concentration in percent of baseline after a one-hour supervised exercise session.

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