Antiviral activities of sulfated polysaccharides isolated from Sphaerococcus coronopifolius (Rhodophytha, Gigartinales) and Boergeseniella thuyoides (Rhodophyta, Ceramiales)

Rhimou Bouhlal, Camille Haslin, Jean-Claude Chermann, Sylvia Colliec-Jouault, Corinne Sinquin, Gaelle Simon, Stephane Cerantola, Hassane Riadi, Nathalie Bourgougnon, Rhimou Bouhlal, Camille Haslin, Jean-Claude Chermann, Sylvia Colliec-Jouault, Corinne Sinquin, Gaelle Simon, Stephane Cerantola, Hassane Riadi, Nathalie Bourgougnon

Abstract

Water-soluble sulfated polysaccharides isolated from two red algae Sphaerococcus coronopifolius (Gigartinales, Sphaerococcaceae) and Boergeseniella thuyoides (Ceramiales, Rhodomelaceae) collected on the coast of Morocco inhibited in vitro replication of the Human Immunodeficiency Virus (HIV) at 12.5 μg/mL. In addition, polysaccharides were capable of inhibiting the in vitro replication of Herpes simplex virus type 1 (HSV-1) on Vero cells values of EC₅₀ of 4.1 and 17.2 μg/mL, respectively. The adsorption step of HSV-1 to the host cell seems to be the specific target for polysaccharide action. While for HIV-1, these results suggest a direct inhibitory effect on HIV-1 replication by controlling the appearance of the new generations of virus and potential virucidal effect. The polysaccharides from S. coronopifolius (PSC) and B. thuyoides (PBT) were composed of galactose, 3,6-anhydrogalactose, uronics acids, sulfate in ratios of 33.1, 11.0, 7.7 and 24.0% (w/w) and 25.4, 16.0, 3.2, 7.6% (w/w), respectively.

Keywords: B. thuyoides; Herpes simplex virus; S. coronopifolius; antiviral activity; chemical composition; human immunodeficiency virus; sulfated polysaccharide.

Figures

Figure 1
Figure 1
FTIR spectrum of polysaccharide of S. coronopifolius.
Figure 2
Figure 2
FTIR spectrum of polysaccharide of B. thuyoides.
Figure 3
Figure 3
Anti-HSV-1 activities of polysaccharides at 72 h. Antiviral activity is expressed as the percentage of viable virus-infected cells (% of protection). Cytotoxic activity is observed as the percentage of viable mock-infected cells (% of destruction).

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