A novel strain of Lactobacillus mucosae isolated from a Gaotian villager improves in vitro and in vivo antioxidant as well as biological properties in D-galactose-induced aging mice
Xiaomin Yu, Shengjie Li, Dong Yang, Liang Qiu, Yaoping Wu, Dengyuan Wang, Nagendra P Shah, Feng Xu, Hua Wei, Xiaomin Yu, Shengjie Li, Dong Yang, Liang Qiu, Yaoping Wu, Dengyuan Wang, Nagendra P Shah, Feng Xu, Hua Wei
Abstract
Twelve isolates isolated from the gastrointestinal tracts of Gaotian villagers in China, who had a lifespan of 92 yr, were examined for their antioxidants using free radical scavenging activity and 2,2-diphenyl-1-picrylhydrazyl. Three strains (i.e., Lactobacillus mucosae LMU1001, and Lactobacillus plantarum LPL0902 and LPL0302) were selected as candidates to prepare yogurt for testing their antioxidants in a model of d-galactose-induced aging mice, with vitamin C as a positive control. The results showed that L. mucosae LMU1001 was the best strain, which had similar in vivo antioxidant activity as vitamin C. A significant increase was found in the activities of glutathione peroxidase in serum and total superoxide dismutase in the liver, and a decrease in the level of malondialdehyde in serum. Regarding mRNA expression level detected quantitatively by real-time PCR, we observed that L. mucosae LMU1001 significantly upregulated antioxidant genes (i.e., MT1A and MT1M in HT-29 and Caco-2) and those genes (i.e., MT1, MT2, GPx1, and GPx2) in the intestinal tract of the model mice. Hence, this strain could be considered as a potential probiotic lactic acid bacterium for improving antioxidant levels in functional foods.
Keywords: 2,2-diphenyl-1-picrylhydrazyl; antioxidant activity; d-galactose-induced aging mice; hydroxyl radical; reducing power.
Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.
Source: PubMed