The Effect of Submucosal Injection of Plasma-Rich Platelets on Blood Inflammatory Markers for Patients with Bimaxillary Protrusion Undergoing Orthodontic Treatment

Trefa Mohammed Ali Mahmood, Omar Fawzi Chawshli, Trefa Mohammed Ali Mahmood, Omar Fawzi Chawshli

Abstract

Objectives: The present study aims to reveal the systemic effects of submucosal injection of plasma-rich platelets (PRP) on blood inflammatory markers which was used in an attempt to reduce the retraction time of the upper canine following extraction of upper maxillary premolars for patients with bimaxillary protrusion.

Hypothesis: No change on comparing the values of blood inflammatory markers before and after submucosal injection of PRP.

Methods: Eighteen female patients with bimaxillary protusion were selected from patients seeking orthodontic treatment from the College of Dentistry/University of Sulaimai, whose maxillary and mandibular first premolars were decided to be extracted after proper diagnosis. Thirty-three blood markers (twenty hematological and thirteen biochemical markers) were estimated before orthodontic bracketing, 24 hours and 7 days following submucosal injection of PRP (5 cc) to reveal the systematic effect of PRP on blood inflammatory markers that were used in an attempt to reduce the retraction time of the upper canine following extraction of upper maxillary premolars for patients with bimaxillary protrusion.

Results: The results indicate nonsignificant differences in the values of all blood markers except for gamma GT (GGT), PDWa, serum albumin, serum total protein, and total calcium. Gamma level significantly increased for both test intervals. On the other hand, there was a significant drop in the value of PDWa while for alkaline phosphatase, there was a drop within the first 24 hr of PRP injection while after 7 days the value was significantly increased. On the other hand, there was a drop in the level of serum albumin, while there was an increase in the serum total protein and total calcium.

Conclusion: Submucosal injection of PRP could lead to systematic alteration of blood parameters including ALK phosphatase, gamma GT, serum albumin, and serum total protein, which may be related to liver function in addition to increase in the level of PDWa and serum calcium. We present evidence that PRP contains and may trigger systemic effect. Thus, further investigation is recommended to follow up the patient for a longer period of time and on a larger sample. This trial is registered with U1111-1221-8829 by Sri Lanka Clinical Trial Registry, SLCTR/2018/040, and No. 64 on 6th August 2018 at the local clinical studies database, College of Dentistry.

Conflict of interest statement

The authors declare that they have no conflicts of interest.

Copyright © 2019 Trefa Mohammed Ali Mahmood and Omar Fawzi Chawshli.

Figures

Figure 1
Figure 1
Bracketing.
Figure 2
Figure 2
ROTIXA 500 RS Hettich floor-standing centrifuge was used, producing high concentration PRP (5 times the concentration in whole blood).
Figure 3
Figure 3
PRP preparation.
Figure 4
Figure 4
Site of PRP injection and force application.

References

    1. Mangal U. Influence of platelet rich plasma on orthodontic tooth movement: a review. Biomedical and Pharmacology Journal. 2017;10(3):1463–1468. doi: 10.13005/bpj/1253.
    1. Zotti F., Albanese M., Rodella L., Nocini P. Platelet-rich plasma in treatment of temporomandibular joint dysfunctions: narrative review. International Journal of Molecular Sciences. 2019;20(2):p. 277. doi: 10.3390/ijms20020277.
    1. Alves R., Grimalt R. A review of platelet-rich plasma: history, biology, mechanism of action, and classification. Skin Appendage Disorders. 2018;4(1):18–24. doi: 10.1159/000477353.
    1. Marx R. E. Platelet-rich plasma (PRP): what is PRP and what is not PRP? Implant Dentistry. 2001;10(4):225–228. doi: 10.1097/00008505-200110000-00002.
    1. Marx R. E. Platelet-rich plasma: evidence to support its use. Journal of Oral and Maxillofacial Surgery. 2004;62(4):489–496. doi: 10.1016/j.joms.2003.12.003.
    1. Güleç A., Bakkalbaşı B. Ç., Cumbul A., Uslu Ü., Alev B., Yarat A. Effects of local platelet-rich plasma injection on the rate of orthodontic tooth movement in a rat model: a histomorphometric study. American Journal of Orthodontics and Dentofacial Orthopedics. 2017;151(1):92–104. doi: 10.1016/j.ajodo.2016.05.016.
    1. Yashin D., Dalci O., Almuzian M., et al. Markers in blood and saliva for prediction of orthodontically induced inflammatory root resorption: a retrospective case controlled-study. Progress in Orthodontics. 2017;18(1):p. 27. doi: 10.1186/s40510-017-0176-y.
    1. Ileri Z., Akin M., Erdur E. A., Dagi H. T., Findik D. Bacteremia after piezocision. American Journal of Orthodontics and Dentofacial Orthopedics. 2014;146(4):430–436. doi: 10.1016/j.ajodo.2014.06.009.
    1. Azeem M., Ul Haq A., Ilyas M., et al. Bacteremia after micro-osteoperforation. International Orthodontics. 2018;16(3):463–469. doi: 10.1016/j.ortho.2018.06.001.
    1. Greene J. C., Vermillion J. R. The oral hygiene index: a method for classifying oral hygiene status. The Journal of American Dental Association. 1960;61(2):172–179. doi: 10.14219/jada.archive.1960.0177.
    1. Hegde R. Effects of periodontal therapy on systemic markers in healthy patients. Journal of Dentistry and Oral Biology. 2017;2(8):p. 1055.
    1. Kanuru R., Kolasani B., Narayana V., Indukuri R., Azaneen M., Babu F. Comparison of canine retraction by in vivo method using four brands of elastomeric power chain. Journal of International Society of Preventive and Community Dentistry. 2014;4(4):32–37. doi: 10.4103/2231-0762.144586.
    1. Fitzpatrick J., Bulsara M. K., McCrory P. R., Richardson M. D., Zheng M. H. Analysis of platelet-rich plasma extraction: variations in platelet and blood components between 4 common commercial kits. Orthopaedic Journal of Sports Medicine. 2017;5(1) doi: 10.1177/2325967116675272.232596711667527
    1. Dhurat R., Sukesh M. S. Principles and methods of preparation of platelet-rich plasma: a review and author’s perspective. Journal of Cutaneous and Aesthetic Surgery. 2014;7(4):189–197. doi: 10.4103/0974-2077.150734.
    1. Liou E. W. The development of submucosal injection of platelet rich plasma for accelerating orthodontic tooth movement and preserving pressure side alveolar bone. APOS Trends in Orthodontics. 2016;6(1):5–11. doi: 10.4103/2321-1407.173725.
    1. Amin A. A., Arf A. N., Rashid Z. J. Angle’s classification of first molar occlusion among patients attending a private orthodontic clinic in Sulaimani City. Sulaimani Dental Journal. 2014;1(2):91–93. doi: 10.17656/sdj.10027.
    1. MacLaine J. K., Rabie A. B. M., Wong R. Does orthodontic tooth movement cause an elevation in systemic inflammatory markers? The European Journal of Orthodontics. 2010;32(4):435–440. doi: 10.1093/ejo/cjp108.
    1. Bilgic F., Akinci Sozer O., Ozcan O., Gurpinar A. B., Yilmaz H., Ay Y. Evaluation of inflammation during fixed orthodontic treatment. Archives of Oral Biology. 2016;71:54–58. doi: 10.1016/j.archoralbio.2016.07.004.
    1. Koenig G., Seneff S. Gamma-glutamyltransferase: a predictive biomarker of cellular antioxidant inadequacy and disease risk. Disease markers. 2015;2015:18. doi: 10.1155/2015/818570.818570
    1. Wang J., Xing L., Pu J., et al. Association between gamma-glutamyl transferase and coronary atherosclerotic plaque vulnerability: an optical coherence tomography study. BioMed Research International. 2019;2019:11. doi: 10.1155/2019/9602783.9602783
    1. Fischbach F. T. Manual of Laboratory & Diagnostic Tests. 7th. Philadelphia, PA, USA: Lippincott Williams & Wilkins; 2003.
    1. Huizen J. What do abnormal ALP levels mean? Medical News Today. 2018, .
    1. Chien S. C., Chen C. Y., Lin C. F., Yeh H. I. Critical appraisal of the role of serum albumin in cardiovascular disease. Biomarker Research. 2017;5(1):p. 31. doi: 10.1186/s40364-017-0111-x.
    1. Cabrerizo S., Cuadras D., Gomez-Busto F., Artaza-Artabe I., Marín-Ciancas F., Malafarina V. Serum albumin and health in older people: review and meta analysis. Maturitas. 2015;81(1):17–27. doi: 10.1016/j.maturitas.2015.02.009.
    1. Melillo A. Applications of serum protein electrophoresis in exotic pet medicine. Veterinary Clinics of North America: Exotic Animal Practice. 2013;16(1):211–225. doi: 10.1016/j.cvex.2012.11.002.
    1. Madhuvanthi M., Lathadevi G. V. Serum proteins alteration in association with body mass index in human volunteers. Journal of Clinical and Diagnostic Research JCDR. 2016;10(6):CC05–CC07. doi: 10.7860/JCDR/2016/18278.8047.
    1. Eske J. What to know about the protein test and results. Medical News Today, MediLexicon, Intl., 2019, .
    1. Clinical and Laboratory Standards Institute. C31-A2 Ionized Calcium Determinations: Precollection Variables, Specimen Choice, Collection, and Handling; Approved Guideline. 2nd. Vol. 12. Wayne, PA, USA: Clinical and Laboratory Standards Institute; 2001.
    1. Salem N. A., Hamza A., Alnahdi H., Ayaz N. Biochemical and molecular mechanisms of platelet-rich plasma in ameliorating liver fibrosis induced by dimethylnitrosurea. Cellular Physiology and Biochemistry. 2018;47(6):2331–2339. doi: 10.1159/000491544.
    1. Takahashi K., Murata S., Ohkohchi N. Platelets and liver regeneration. Austin Journal of Surgery. 2014;1(4):p. 1019.

Source: PubMed

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