Integrating muscle cell biochemistry and whole-body physiology in humans:(31)P-MRS data from the InSight trial

Lindsay M Edwards, Graham J Kemp, Renee M Dwyer, Justin T Walls, Huddy Fuller, Steven R Smith, Conrad P Earnest, Lindsay M Edwards, Graham J Kemp, Renee M Dwyer, Justin T Walls, Huddy Fuller, Steven R Smith, Conrad P Earnest

Abstract

We acquired (31)P-MRS data from skeletal muscle of subjects of mixed gender and ethnicity, combined with a panel of physiological characteristics, and tested several long-standing hypotheses regarding relationships between muscle cell biochemistry and whole-body physiology with unusually high statistical power. We hypothesized that i) whole-body VO(2)max would correlate with muscle respiratory capacity, ii) resting muscle phosphocreatine concentration ([PCr]) would negatively correlate with delta efficiency and iii) muscle mitochondrial function would positively correlate with both resting VO(2) and total daily energy expenditure (TDEE). Muscle respiratory capacity explained a quarter of the variation in VO(2)max (r(2) = 26, p < .001, n = 87). There was an inverse correlation between muscle [PCr] and delta efficiency (r = -23, p = 046, n = 87). There was also a correlation between [PCr] recovery halftime and TDEE (r = -23, p = 035, n = 87). Our data not only provide insights into muscle cell chemistry and whole-body physiology but our mixed cohort means that our findings are broadly generalizable.

Figures

Figure 1. Correlations between 31 P-MRS estimates…
Figure 1. Correlations between 31P-MRS estimates of mitochondrial function and either absolute (left column) or mass-adjusted (right column) VO2max (n = 86).
Absolute VO2max is in L min−1, mass-adjusted VO2max is in mL min−1 kg−1.
Figure 2. Correlation between PCr 1/2t (see…
Figure 2. Correlation between PCr1/2t (see main text for details) and total daily energy expenditure (TDEE); relationship is significant at p < .05.
Figure 3. Correlations between 31 P-MRS estimates…
Figure 3. Correlations between 31P-MRS estimates of mitochondrial function and percent body fat (left column) or fat mass (right column, in kg) (n = 86).
Figure 4. Principle components analysis scores biplot…
Figure 4. Principle components analysis scores biplot for 31P-MRS data from 87 subjects of mixed gender and ethnicity.

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