Effects of an orally supplemented probiotic on the autophagy protein LC3 and Beclin1 in placentas undergoing spontaneous delivery during normal pregnancy

Ping Yang, Zhe Li, Kian Deng Tye, Yuyi Chen, Tong Lu, Zonglin He, Juan Zhou, Xiaomin Xiao, Ping Yang, Zhe Li, Kian Deng Tye, Yuyi Chen, Tong Lu, Zonglin He, Juan Zhou, Xiaomin Xiao

Abstract

Background: Probiotic supplementation has been shown to be beneficial and is now widely promoted as an auxiliary medicine for maternal health, but the underlying mechanism is still unclear. Thus, this study aimed to explore the effects of probiotic supplementation on the placental autophagy-related proteins LC3 and Beclin1.

Method: A population-based cohort of specimens was collected under sterile conditions from 37 healthy nulliparous pregnant women who underwent systemic examination and delivered at the First Affiliated Hospital of Jinan University (Guangzhou, China). At 32 weeks of gestation, the pregnant women in the probiotic group were orally supplemented with golden bifid, and the pregnant women in the control group received no probiotic. Pregnant women with pregnancy-associated complications were excluded in the follow-up period, and 25 pregnant women undergoing spontaneous delivery were enrolled. The placental tissue specimens were collected at term. Western blotting was used to detect the protein expression, and qRT-PCR was used to detect the mRNA expression of the placental autophagy-related proteins LC3 and Beclin1.

Results: ①There was no significant difference in the expression levels of either LC3 or Beclin1 protein between the two groups (P > 0.05). ②Probiotic supplementation induced a modest but not significant decrease in the content of LC3-mRNA with a significant decrease in the content of Beclin1-mRNA (P < 0.05).

Conclusion: Our study indicates that probiotic supplementation may reduce Beclin1-mRNA levels.

Keywords: Autophagy; Normal pregnancy; Probiotic; Spontaneous delivery.

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Technical flow chart for screening research subjects
Fig. 2
Fig. 2
representative western blot for LC3 and Beclin1 on term placenta of two groups. Note: Control Group:P1-P15、P28、P32、P36、P37 Probiotic Group:P17-P27、P29-P31、P33-P35
Fig. 3
Fig. 3
The analysis of data from optical density values of Western blotting bands of two groups. Note: “*” means the difference is statistically significant (p < 0.05)
Fig. 4
Fig. 4
Comparison of autophagy-related proteins LC3-mRNA and Beclin1-mRNA on term placenta of two groups. a: LC3 protein amplification curve (b): LC3 protein melt curve (c): Beclin1 protein amplification curve (d): Beclin1 protein melt curve
Fig. 5
Fig. 5
Rt-PCR amplification curve and melt curve of LC3 and Beclin1 mRNA on term placenta of two groups

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Source: PubMed

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