The infant nasopharyngeal microbiome impacts severity of lower respiratory infection and risk of asthma development

Shu Mei Teo, Danny Mok, Kym Pham, Merci Kusel, Michael Serralha, Niamh Troy, Barbara J Holt, Belinda J Hales, Michael L Walker, Elysia Hollams, Yury A Bochkov, Kristine Grindle, Sebastian L Johnston, James E Gern, Peter D Sly, Patrick G Holt, Kathryn E Holt, Michael Inouye, Shu Mei Teo, Danny Mok, Kym Pham, Merci Kusel, Michael Serralha, Niamh Troy, Barbara J Holt, Belinda J Hales, Michael L Walker, Elysia Hollams, Yury A Bochkov, Kristine Grindle, Sebastian L Johnston, James E Gern, Peter D Sly, Patrick G Holt, Kathryn E Holt, Michael Inouye

Abstract

The nasopharynx (NP) is a reservoir for microbes associated with acute respiratory infections (ARIs). Lung inflammation resulting from ARIs during infancy is linked to asthma development. We examined the NP microbiome during the critical first year of life in a prospective cohort of 234 children, capturing both the viral and bacterial communities and documenting all incidents of ARIs. Most infants were initially colonized with Staphylococcus or Corynebacterium before stable colonization with Alloiococcus or Moraxella. Transient incursions of Streptococcus, Moraxella, or Haemophilus marked virus-associated ARIs. Our data identify the NP microbiome as a determinant for infection spread to the lower airways, severity of accompanying inflammatory symptoms, and risk for future asthma development. Early asymptomatic colonization with Streptococcus was a strong asthma predictor, and antibiotic usage disrupted asymptomatic colonization patterns. In the absence of effective anti-viral therapies, targeting pathogenic bacteria within the NP microbiome could represent a prophylactic approach to asthma.

Copyright © 2015 Elsevier Inc. All rights reserved.

Figures

Graphical abstract
Graphical abstract
Figure 1
Figure 1
Bacterial Composition of 1,021 Nasopharyngeal Aspirates Collected from 234 Infants during Periods of Respiratory Health and Disease (A) Frequency of the most abundant phyla and genera (comprising 99.9% of reads). (B) Clustering of samples into microbiome profile groups (MPGs) based on relative abundance of the six most common genera. Colored bars indicate MPGs, labeled by their dominant genus: Moraxella (red), Corynebacterium (blue), Alloiococcus (green), Staphylococcus (purple), Haemophilus (yellow), and Streptococcus (orange). (C) Weekly frequencies of MPGs among healthy samples, collected during planned visits at approximately 2, 6, and 12 months of age and following at least 4 weeks without symptoms of acute respiratory infection (ARI). (D) Weekly frequencies of each MPG among ARI samples. (E) Odds ratios for association of MPGs with ARI symptoms, adjusted for age, gender, season, number of prior infections, antibiotics intake, mother’s antibiotics intake, delivery mode, and breastfeeding; with and without adjustment for detection of common viruses (RSV, HRV).
Figure 2
Figure 2
Impact of Environmental Factors on Relative Abundances of Major Genera of the NP Microbiome (A–D) Squares, odds ratios; filled squares, healthy samples; empty squares, infection samples; bars, 95% confidence intervals; ∗p < 0.1, ∗∗p < 0.05, ∗∗∗p < 0.01. Associations are estimated using logistic regression and adjusted for age: (A) day care attendance (yes versus no, 12-month samples), (B) co-habiting with siblings, (C) antibiotics intake in the 4 weeks preceding NP sample collection, (D) season (spring-summer versus autumn-winter). (E) Impact of prior ARI, estimated using proportional odds ordinal logistic regression and categorized as 0, 1, or ≥ 2 ARI. (F) Seasonal patterns: top, mean maximum and minimum temperatures in study location (Perth); bottom, monthly proportions of samples in each microbiome profile group (MPG) for infection and healthy samples.
Figure 3
Figure 3
Symptoms of Lower Respiratory Illness during the First Year of Life Are Associated with Viruses Present during the Infection and Predict Chronic Wheeze at 5 Years of Age (A and B) Kaplan-Meier survival curves for age (days) at (A) first febrile LRI and (B) first HRV-C wheezy LRI, stratified by chronic wheeze status at 5 years. p values shown were estimated using Cox proportional hazards models, adjusted for gender and maternal and paternal history of atopic disease. Shaded areas indicate 95% confidence intervals. (C) Frequencies of fever and wheeze symptoms during LRI, in which HRV-C and/or RSV were detected. Total numbers are: HRV-C only, n = 79; HRV-C and RSV, n = 14; RSV only, n = 22. (D) Cross-tabulation of individuals according to their experience of LRI during infancy; percentages in brackets indicate frequency of chronic wheeze at 5 years.
Figure 4
Figure 4
Impact of Early Colonization on Age at First Respiratory Infection (A) Microbiome profile group (MPG) transitions between healthy samples (T1) and the next sequenced infection (T2). Cell numbers indicate the number of times the respective transition from T1 to T2 was observed in the dataset; cells are colored to indicate the row proportions as per legend. (B–D) Kaplan-Meier survival curves for age (days) of first (B) acute respiratory illness (ARI), (C) upper respiratory illness (URI), and (D) lower respiratory illness (LRI), stratified according to the MPG of the first healthy sample (collected by 9 weeks of age and prior to any infection, n = 160). Cox proportional hazards models were adjusted for age, gender, season, virus status in the early healthy sample, and virus status at the first event. Shaded areas indicate 95% confidence intervals.
Figure 5
Figure 5
Predictors of Chronic Wheeze at Age 5 (A) Streptococcus abundance among healthy samples collected by 9 weeks of age, broken down by microbiome profile group (MPG). (B) Adjusted odds ratio (OR, squares) and 95% confidence intervals (bars) for association between chronic wheeze at age 5 and high (> 20%) abundance of Streptococcus in the first healthy NP sample; p values and sample sizes (n) are indicated; individuals who experienced an infection prior to first healthy NP sample collection were excluded. (C) Distribution of microbial events during infancy that were identified as risk factors for chronic wheeze at 5 years of age, stratified according to atopic status by age 2. fLRI, febrile LRI; wHRV-C, HRV-C LRI accompanied by wheeze; Strep, > 20% Streptococcus abundance in healthy NP sample taken in by 9 weeks old and prior to any ARI; unknown Strep, no such NP sample available (mainly due to ARI before 9 weeks of age); unknown LRI, incomplete viral/symptom profiling for LRI. Size of pie chart is proportional to the number of infants in each condition.

References

    1. Arrieta M.C., Finlay B. The intestinal microbiota and allergic asthma. J. Infect. 2014;69(1):S53–S55.
    1. Biesbroek G., Wang X., Keijser B.J., Eijkemans R.M., Trzciński K., Rots N.Y., Veenhoven R.H., Sanders E.A., Bogaert D. Seven-valent pneumococcal conjugate vaccine and nasopharyngeal microbiota in healthy children. Emerg. Infect. Dis. 2014;20:201–210.
    1. Bisgaard H., Hermansen M.N., Buchvald F., Loland L., Halkjaer L.B., Bønnelykke K., Brasholt M., Heltberg A., Vissing N.H., Thorsen S.V. Childhood asthma after bacterial colonization of the airway in neonates. N. Engl. J. Med. 2007;357:1487–1495.
    1. Bisgaard H., Hermansen M.N., Bønnelykke K., Stokholm J., Baty F., Skytt N.L., Aniscenko J., Kebadze T., Johnston S.L. Association of bacteria and viruses with wheezy episodes in young children: prospective birth cohort study. BMJ. 2010;341:c4978.
    1. Bochkov Y.A., Gern J.E. Clinical and molecular features of human rhinovirus C. Microbes Infect. 2012;14:485–494.
    1. Bogaert D., Keijser B., Huse S., Rossen J., Veenhoven R., van Gils E., Bruin J., Montijn R., Bonten M., Sanders E. Variability and diversity of nasopharyngeal microbiota in children: a metagenomic analysis. PLoS ONE. 2011;6:e17035.
    1. Bokulich N.A., Subramanian S., Faith J.J., Gevers D., Gordon J.I., Knight R., Mills D.A., Caporaso J.G. Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing. Nat. Methods. 2013;10:57–59.
    1. Brockson M.E., Novotny L.A., Jurcisek J.A., McGillivary G., Bowers M.R., Bakaletz L.O. Respiratory syncytial virus promotes Moraxella catarrhalis-induced ascending experimental otitis media. PLoS ONE. 2012;7:e40088.
    1. Caporaso J.G., Kuczynski J., Stombaugh J., Bittinger K., Bushman F.D., Costello E.K., Fierer N., Peña A.G., Goodrich J.K., Gordon J.I. QIIME allows analysis of high-throughput community sequencing data. Nat. Methods. 2010;7:335–336.
    1. de Vries S.P., Bootsma H.J., Hays J.P., Hermans P.W. Molecular aspects of Moraxella catarrhalis pathogenesis. Microbiol. Mol. Biol. Rev. 2009;73:389–406.
    1. Edgar R.C. Search and clustering orders of magnitude faster than BLAST. Bioinformatics. 2010;26:2460–2461.
    1. Gern J.E. Rhinovirus and the initiation of asthma. Curr. Opin. Allergy Clin. Immunol. 2009;9:73–78.
    1. Gisselsson-Solén M., Henriksson G., Hermansson A., Melhus A. Risk factors for carriage of AOM pathogens during the first 3 years of life in children with early onset of acute otitis media. Acta Otolaryngol. 2014;134:684–690.
    1. Hales B.J., Chai L.Y., Elliot C.E., Pearce L.J., Zhang G., Heinrich T.K., Smith W.A., Kusel M.M., Holt P.G., Sly P.D., Thomas W.R. Antibacterial antibody responses associated with the development of asthma in house dust mite-sensitised and non-sensitised children. Thorax. 2012;67:321–327.
    1. Hilty M., Burke C., Pedro H., Cardenas P., Bush A., Bossley C., Davies J., Ervine A., Poulter L., Pachter L. Disordered microbial communities in asthmatic airways. PLoS ONE. 2010;5:e8578.
    1. Holt P.G., Sly P.D. Viral infections and atopy in asthma pathogenesis: new rationales for asthma prevention and treatment. Nat. Med. 2012;18:726–735.
    1. Holt P.G., Rowe J., Kusel M., Parsons F., Hollams E.M., Bosco A., McKenna K., Subrata L., de Klerk N., Serralha M. Toward improved prediction of risk for atopy and asthma among preschoolers: a prospective cohort study. J. Allergy Clin. Immunol. 2010;125:653–659, e1, e7.
    1. Holt P.G., Strickland D.H., Hales B.J., Sly P.D. Defective respiratory tract immune surveillance in asthma: a primary causal factor in disease onset and progression. Chest. 2014;145:370–378.
    1. Jackson D.J., Gangnon R.E., Evans M.D., Roberg K.A., Anderson E.L., Pappas T.E., Printz M.C., Lee W.M., Shult P.A., Reisdorf E. Wheezing rhinovirus illnesses in early life predict asthma development in high-risk children. Am. J. Respir. Crit. Care Med. 2008;178:667–672.
    1. Kusel M.M., de Klerk N.H., Holt P.G., Kebadze T., Johnston S.L., Sly P.D. Role of respiratory viruses in acute upper and lower respiratory tract illness in the first year of life: a birth cohort study. Pediatr. Infect. Dis. J. 2006;25:680–686.
    1. Kusel M.M., de Klerk N.H., Kebadze T., Vohma V., Holt P.G., Johnston S.L., Sly P.D. Early-life respiratory viral infections, atopic sensitization, and risk of subsequent development of persistent asthma. J. Allergy Clin. Immunol. 2007;119:1105–1110.
    1. Kusel M.M., de Klerk N., Holt P.G., Sly P.D. Antibiotic use in the first year of life and risk of atopic disease in early childhood. Clin. Exp. Allergy. 2008;38:1921–1928.
    1. Kusel M.M., Kebadze T., Johnston S.L., Holt P.G., Sly P.D. Febrile respiratory illnesses in infancy and atopy are risk factors for persistent asthma and wheeze. Eur. Respir. J. 2012;39:876–882.
    1. Lee W.M., Lemanske R.F., Jr., Evans M.D., Vang F., Pappas T., Gangnon R., Jackson D.J., Gern J.E. Human rhinovirus species and season of infection determine illness severity. Am. J. Respir. Crit. Care Med. 2012;186:886–891.
    1. Magoč T., Salzberg S.L. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics. 2011;27:2957–2963.
    1. McDonald D., Price M.N., Goodrich J., Nawrocki E.P., DeSantis T.Z., Probst A., Andersen G.L., Knight R., Hugenholtz P. An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea. ISME J. 2012;6:610–618.
    1. Oddy W.H., de Klerk N.H., Sly P.D., Holt P.G. The effects of respiratory infections, atopy, and breastfeeding on childhood asthma. Eur. Respir. J. 2002;19:899–905.
    1. Penders J., Kummeling I., Thijs C. Infant antibiotic use and wheeze and asthma risk: a systematic review and meta-analysis. Eur. Respir. J. 2011;38:295–302.
    1. Ravel J., Gajer P., Abdo Z., Schneider G.M., Koenig S.S., McCulle S.L., Karlebach S., Gorle R., Russell J., Tacket C.O. Vaginal microbiome of reproductive-age women. Proc. Natl. Acad. Sci. USA. 2011;108(1):4680–4687.
    1. Revai K., McCormick D.P., Patel J., Grady J.J., Saeed K., Chonmaitree T. Effect of pneumococcal conjugate vaccine on nasopharyngeal bacterial colonization during acute otitis media. Pediatrics. 2006;117:1823–1829.
    1. Rollins D.R., Beuther D.A., Martin R.J. Update on infection and antibiotics in asthma. Curr. Allergy Asthma Rep. 2010;10:67–73.
    1. Schaumburg F., Alabi A.S., Mombo-Ngoma G., Kaba H., Zoleko R.M., Diop D.A., Mackanga J.R., Basra A., Gonzalez R., Menendez C. Transmission of Staphylococcus aureus between mothers and infants in an African setting. Clin. Microbiol. Infect. 2014;20:O390–O396.
    1. Semic-Jusufagic A., Belgrave D., Pickles A., Telcian A.G., Bakhsoliani E., Sykes A., Simpson A., Johnston S.L., Custovic A. Assessing the association of early life antibiotic prescription with asthma exacerbations, impaired antiviral immunity, and genetic variants in 17q21: a population-based birth cohort study. Lancet Respir Med. 2014;2:621–630.
    1. Sly P.D., Boner A.L., Björksten B., Bush A., Custovic A., Eigenmann P.A., Gern J.E., Gerritsen J., Hamelmann E., Helms P.J. Early identification of atopy in the prediction of persistent asthma in children. Lancet. 2008;372:1100–1106.
    1. Spaniol V., Troller R., Schaller A., Aebi C. Physiologic cold shock of Moraxella catarrhalis affects the expression of genes involved in the iron acquisition, serum resistance and immune evasion. BMC Microbiol. 2011;11:182.
    1. Stein R.T., Martinez F.D. Respiratory syncytial virus and asthma: still no final answer. Thorax. 2010;65:1033–1034.
    1. Tai A., Tran H., Roberts M., Clarke N., Wilson J., Robertson C.F. The association between childhood asthma and adult chronic obstructive pulmonary disease. Thorax. 2014;69:805–810.
    1. Tano K., von Essen R., Eriksson P.O., Sjöstedt A. Alloiococcus otitidis—otitis media pathogen or normal bacterial flora? APMIS. 2008;116:785–790.
    1. Verhaegh S.J., Snippe M.L., Levy F., Verbrugh H.A., Jaddoe V.W., Hofman A., Moll H.A., van Belkum A., Hays J.P. Colonization of healthy children by Moraxella catarrhalis is characterized by genotype heterogeneity, virulence gene diversity and co-colonization with Haemophilus influenzae. Microbiology. 2011;157:169–178.
    1. Vissers M., de Groot R., Ferwerda G. Severe viral respiratory infections: are bugs bugging? Mucosal Immunol. 2014;7:227–238.
    1. Weinstock G.M. Genomic approaches to studying the human microbiota. Nature. 2012;489:250–256.
    1. Wu P., Hartert T.V. Evidence for a causal relationship between respiratory syncytial virus infection and asthma. Expert Rev. Anti Infect. Ther. 2011;9:731–745.
    1. Zhou Y., Mihindukulasuriya K.A., Gao H., La Rosa P.S., Wylie K.M., Martin J.C., Kota K., Shannon W.D., Mitreva M., Sodergren E., Weinstock G.M. Exploration of bacterial community classes in major human habitats. Genome Biol. 2014;15:R66.

Source: PubMed

Sponzoři a spolupracovníci

Zdravotní podmínky

Drogové intervence

3
Předplatit