Angiogenic effects of cryosurgery with liquid nitrogen on the normal skin of rats, through morphometric study

Camila Bianco Pimentel, Aparecida Machado de Moraes, Maria Letícia Cintra, Camila Bianco Pimentel, Aparecida Machado de Moraes, Maria Letícia Cintra

Abstract

Background: Cryosurgery is an efficient therapeutic technique used to treat benign and malignant cutaneous diseases. The primary active mechanism of cryosurgery is related to vascular effects on treated tissue. After a cryosurgical procedure, exuberant granulation tissue is formed at the injection site, probably as a result of angiogenic stimulation of the cryogen and inflammatory response, particularly in endothelial cells.

Objective: To evaluate the angiogenic effects of freezing, as part of the phenomenon of healing rat skin subjected to previous injury.

Methods: Two incisions were made in each of the twenty rats, which were divided randomly into two groups of ten. After 3 days, cryosurgery with liquid nitrogen was performed in one of incisions. The rats' samples were then collected, cut and stained to conduct histopathological examination, to assess the local angiogenesis in differing moments and situations.

Results: It was possible to demonstrate that cryosurgery, in spite of promoting cell death and accentuated local inflammation soon after its application, induces quicker cell proliferation in the affected tissue and maintenance of this rate in a second phase, than in tissue healing without this procedure.

Conclusions: These findings, together with the knowledge that there is a direct relationship between mononuclear cells and neovascularization (the development of a rich system of new vessels in injury caused by cold), suggest that cryosurgery possesses angiogenic stimulus, even though complete healing takes longer to occur. The significance level for statistical tests was 5% (p<0,05).

Conflict of interest statement

Conflict of Interest: None.

Figures

FIGURE 1
FIGURE 1
A - Comparison of number of cell nuclei among treatments and groups. CA (control area) 7 days after experimental injury(100X);B - Comparison of number of cell nuclei among treatments and groups. TA (treated area) 7 days after experimental injury(100X);C - Comparison of number of cell nuclei among treatments and groups. CA (control area) 14 days after experimental injury(100X);D: Comparison of number of cell nuclei among treatments and groups. TA (treated area) 14 days after experimental injury (100X)
GRAPH 1
GRAPH 1
Graphic representation of the cellularity comparison in the two areas

References

    1. Lizhi N, Kecheng X, Feng M. Cryosurgery for lung câncer. J. Thorac. Dis. 2012;4(4):408–416.
    1. Lucas R. Avaliação e comparação de diferentes técnicas de criocirurgia com nitrogênio líquido em cães da raça Beagle, pela tomografia computadorizada, histologia e imunohistoquímica [tese] São Paulo (SP): Universidade de São Paulo; 2001.
    1. Poziomczyk CS, Köche B, Dornelles Mde A, Dornelles SI. Pain evaluation in the cryosurgery of actinic keratoses. An Bras Dermatol. 2011;86:645–650.
    1. Gage AA. History of cryosurgery. Semin Surg Oncol. 1998;14:99–109.
    1. Gage AA, Baust J. Mechanisms of tissue injury in cryosurgery. Cryobiology. 1998;37:171–186.
    1. Yamaguchi Y, Yoshikawa K. Cutaneous Wound healing: an update. J Dermatol. 2001;28:521–534.
    1. Belman S, Adams-Ray J. Vascular reactions after experimental cold injury. Angiology. 1956;7:339–367.
    1. Carmeliet P. Angiogenesis in health and disease. Nat Med. 2003;9:653–660.
    1. Calvin M. Cutaneous wound repair. Wounds. 1998;10:12–32.
    1. Fox SB, Harris AL. Histological quantitation of tumor angiogenesis. APMIS. 2004;112:413–430.
    1. Fina L, Molgaard HV, Robertson D, Bradley NJ, Monaghan P, Delia D, et al. Expression of the CD34 gene in vascular endothelial cells. Blood. 1990;75:2417–2426.

Source: PubMed

Sponzoři a spolupracovníci

Zdravotní podmínky

Drogové intervence

3
Předplatit