Efficacy of 0.2% tempered chlorhexidine as a pre-procedural mouth rinse: A clinical study

Shantipriya Reddy, M G S Prasad, Sanjay Kaul, K Satish, Sabana Kakarala, Nirjhar Bhowmik, Shantipriya Reddy, M G S Prasad, Sanjay Kaul, K Satish, Sabana Kakarala, Nirjhar Bhowmik

Abstract

Objective: Our objective was to determine the efficacy of pre-procedural rinsing with chlorhexidine in reducing bacterial aerosol contamination during use of ultrasonic scaler and comparing the efficacy of water, non-tempered chlorhexidine and tempered chlorhexidine in reducing bacterial count in aerosols when used as a pre-procedural rinse.

Materials and methods: The study was designed to include 30 systemically healthy patients in different age groups. The patients were divided randomly into 3 groups (I, II, III) of 10 patients each to be administered with sterile water, non tempered chlorhexidine and tempered chlorhexidine, respectively, as a pre-procedural rinse. The aerosol produced by the ultrasonic unit was collected at 3° clock, 6° clock and 12° clock positions on blood agar plates within a range of 4 feet in all the three groups. The blood agar plates were incubated for 48 hours and the total number of colony forming units (CFUs) were counted and statistically analyzed.

Results: The results showed that CFU in group III and group II were significantly reduced when compared to group I with F=1084.92, P<0.001 (ANOVA). Also, CFU in group III was significantly reduced when compared to group II with P<0.001.

Conclusions: Pre-procedural rinse can significantly reduce the viable microbial content of dental aerosols and tempered chlorhexidine was more effective than non-tempered chlorhexidine.

Keywords: Aerosol production; colony forming unit; pre-procedural rinse; tempered chlorhexidine.

Conflict of interest statement

Conflict of Interest: None declared

Figures

Figure 1
Figure 1
Diagramatic depiction of the study design
Figure 2
Figure 2
Microbial colonies formed on an agar plate
Figure 3
Figure 3
CFUs in group I (pre-rinse)
Figure 4
Figure 4
CFUs in group I (post-rinse)
Figure 5
Figure 5
CFUs in group II (post-rinse)
Figure 6
Figure 6
CFUs in group III (post-rinse)
Figure 7
Figure 7
Bar diagram showing CFU's
Figure 8
Figure 8
Scatter diagram showing CFU's

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