Gene Expression Profiles in Patients With Permanent Atrial Fibrillation (AF) Versus Sinus Rhythm (SR)
Comparative Gene Expression Profiles in Patients With Permanent Atrial Fibrillation and Normal Sinus Rhythm
Studie Overzicht
Toestand
Gedetailleerde beschrijving
Mitral valve regurgitation (MR) is the second most common valvular heart disease encountered in adults. Furthermore, atrial fibrillation (AF) is the most common cardiac arrhythmia seen in clinical practice. Overall, 70% of the patients with severe MR are associated with AF independent from etiopathogenesis of MR. AF is clinically divided into three subgroups; 1) paroxysmal AF, occurs as episodes and ends spontaneously, 2) persistent AF, episodes terminate only with medical or electrical cardioversion, and 3) permanent AF, current medical treatments and electrical cardioversion does not restore a normal sinus rhythm. Despite intensive electrophysiological studies, the molecular mechanisms and pathways of AF are still not fully elucidated.
Apoptosis which has distinctive morphological and biochemical characteristics is genetically regulated, active programmed cell death process. It is known that cardiac morphogenesis restore from apoptosis. In addition, apoptosis has an important role in several cardiovascular system pathologies. It has been shown that atrial apoptosis causes numerous arrhythmias including AF. Likewise, in the pilot study which has been performed by our study group, AF is associated with apoptosis by immunohistochemical and DNA fragmentation analysis methods.
The aim of this project is to determine the morphological criteria of apoptosis in atrial tissues of patients with AF by using electron microscopy and immunohistochemistry. Moreover, we will investigate the transcriptional profile of AF associated genes by oligonucleotide microarray method. The gene expression profiles of patients with AF and degenerative MR will be compared with the atrial tissue samples from the patients with degenerative MR who preserve normal sinus rhythm which will serve as controls. In summary the apoptotic pathways would be analyzed at transcriptomic and genomic level. Besides, the pathways that may interfere AF pathophysiology would also be evaluated. The expression profiles of the genes primarily verified by quantitative real time RT-PCR will be further confirmed by translation of end-result proteins determined with Western blot technique. Thus, brand-new clues about physiology of fibrillating atrial cells would be achieved.
Keywords: Atrial fibrillation, apoptosis, oligonucleotide microarray
Studietype
Inschrijving (Verwacht)
Contacten en locaties
Studie Locaties
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Ankara, Kalkoen, 06340
- Werving
- Ankara University Medical Faculty, Department of Cardiovascular Surgery,
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Contact:
- SERKAN DURDU
- Telefoonnummer: +905336373535
- E-mail: serkandurdu@gmail.com
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Onderonderzoeker:
- HILAL OZDAG, Assoc. Prof.
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Onderonderzoeker:
- UMIT OZYURDA, Prof.
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Onderonderzoeker:
- HAKAN GURDAL, Prof.
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Contact:
- GUNSELI CUBUKCUOGLU
- Telefoonnummer: +905336424994
- E-mail: gunselicubukcu@gmail.com
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Hoofdonderzoeker:
- RUCHAN A AKAR, Assoc. Prof.
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Onderonderzoeker:
- ARZU ATALAY, PhD
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Onderonderzoeker:
- NALAN AKYUREK, PhD
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Onderonderzoeker:
- OMER AKYUREK, Prof.
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Onderonderzoeker:
- GUNSELI CUBUKCUOGLU, MSc
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Onderonderzoeker:
- CAGIN ZAIM, MD
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Deelname Criteria
Geschiktheidscriteria
Leeftijden die in aanmerking komen voor studie
Accepteert gezonde vrijwilligers
Geslachten die in aanmerking komen voor studie
Bemonsteringsmethode
Studie Bevolking
Beschrijving
Inclusion Criteria:
- Patients with permanent atrial fibrillation or sinus rhythm and degenerative mitral valve regurgitation who require cardiac surgery
- Pulmonary hypertension (systolic PA > 45 mmHg)
- Left ventricular ejection fraction > 30%
Exclusion Criteria:
- Paroxysmal AF or atrial flutter
- Second or third degree heart block
- Permanent pacemaker
- Wolff-Parkinson-White syndrome
- Brugada syndrome
- Ischemic or rheumatic mitral valve disease
- Dilated cardiomyopathy
- LVEF < 30%
- Infective endocarditis, myocarditis
- Trauma
- Active HBV, HCV, HIV infection
- Chronic renal failure
- Autoimmune diseases
- Vasculitis
- Known genetic disorders
Studie plan
Hoe is de studie opgezet?
Ontwerpdetails
Cohorten en interventies
Groep / Cohort |
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AF
Patients with degenerative mitral valve regurgitation and permanent atrial fibrillation who require mitral valve repair or replacement.
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SR
Patients with degenerative mitral valve regurgitation and maintaining sinus rhythm who require mitral valve repair or replacement.
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Wat meet het onderzoek?
Primaire uitkomstmaten
Uitkomstmaat |
Tijdsspanne |
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Expression profiles of genes related to apoptosis
Tijdsspanne: 6 months
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6 months
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Secundaire uitkomstmaten
Uitkomstmaat |
Tijdsspanne |
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Expression profiles of pro-apoptotic and anti-apoptotic proteins
Tijdsspanne: 6 months
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6 months
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Medewerkers en onderzoekers
Sponsor
Onderzoekers
- Hoofdonderzoeker: RUCHAN A AKAR, Assoc. Prof., Ankara University
Publicaties en nuttige links
Studie record data
Bestudeer belangrijke data
Studie start
Studie voltooiing (Verwacht)
Studieregistratiedata
Eerst ingediend
Eerst ingediend dat voldeed aan de QC-criteria
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Meer informatie
Termen gerelateerd aan deze studie
Aanvullende relevante MeSH-voorwaarden
Andere studie-ID-nummers
- 108S375
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