Detection of Vaccine-Induced Antibodies to Ebola Virus in Oral Fluid

Teresa Lambe, Tommy Rampling, Dhan Samuel, Georgina Bowyer, Katie J Ewer, Navin Venkatraman, Matthew Edmans, Steve Dicks, Adrian V S Hill, Richard S Tedder, Sarah C Gilbert, Teresa Lambe, Tommy Rampling, Dhan Samuel, Georgina Bowyer, Katie J Ewer, Navin Venkatraman, Matthew Edmans, Steve Dicks, Adrian V S Hill, Richard S Tedder, Sarah C Gilbert

Abstract

Blood sampling to assess production of antigen-specific antibodies after immunization is commonly performed, but it presents logistical difficulties for trials carried out during an infectious disease outbreak. In this study, we show that antibodies may be reliably detected in oral fluid collected in a minimally invasive manner without use of sharps. Clinical Trials Registration. NCT02240875.

Keywords: Ebola vaccine; antibodies; oral fluid; serology.

Figures

Figure 1.
Figure 1.
Comparison of antibody levels in paired oral fluid and serum from participants in the EBL001 clinical trial. (A) Immunoglobulin (Ig)M antibody levels correlated well between paired oral fluid and serum as assessed with an IgM capture assay (r = 0.78, P < .0001, 2-tailed nonparametric Spearman's correlation). (B) Immunoglobulin G antibody levels correlated well between paired oral fluid and serum as assessed with an IgG capture assay (r = 0.68, P < .0001, 2-tailed nonparametric Spearman's correlation). (C) IgG antibody levels correlated well between paired oral fluid and serum as assessed with a standardized enzyme-linked immunosorbent assay (r = 0.83, P < .0001, 2-tailed nonparametric Spearman's correlation). Dashed lines indicate assay-dependent cutoff point for sample positivity.

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Source: PubMed

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