Impact of uremia on human adipose tissue phenotype

Abstract

Background: Recognition of adipose-related signaling in surgery is increasing, although direct interrogation of human adipose has been sparse. Few scenarios rival uremia for health impact. We hypothesized that adipose from uremic patients holds a relatively higher adipose-derived hormone and proinflammatory adipokine signature; we simultaneously evaluated the impact of clinical parameters on adipose phenotype.

Materials and methods: Adipose was harvested from surgical patients. Histology and protein analyses were completed for select mediators.

Results: In the overall cohort of 71 patients, the mean age was 63.4 y; 46.4% of patients had diabetes mellitus, 49.2% had hyperlipidemia, and 53.5% had coronary artery disease. Compared with nonuremic patients, uremic patients had one-tenth of the levels of leptin (P < 0.001), one-third of the levels of adiponectin (P < 0.001), and threefold higher levels of resistin (P < 0.001). Females had sixfold higher levels of leptin, 1.5-fold higher levels of adiponectin, and twofold higher levels of tumor necrosis factor alpha but equivalent resistin. There were differences in mediators when stratified by age. In both the obese and nonobese strata, we observed a concordant pattern of association (magnitude or significance) of uremia and leptin, adiponectin, and resistin. No differentials in other mediators emerged on body mass index stratification. Multiple regression analysis for leptin, adiponectin, and resistin (with age, gender, and uremia as independent variables) showed uremia as the highest independent predictor of all the three mediators.

Conclusions: Advanced chronic kidney disease is associated with perturbations in adipose-derived hormones (leptin, adiponectin, and resistin). Adipose adiponectin and leptin (in contrast to reported plasma levels) were lower in uremic patients; there is an inverse correlation between adipose resistin and renal function. Compared with other clinical parameters including body mass index, uremia dominates overall in determining adipose phenotype, highlighting the complex biological interplay between uremia and adipose biology.

Conflict of interest statement

Conflict of interest statement: None of the authors hold any commercial affiliations (including consultancies, stock or equity interests, and patent-licensing arrangements) that are a conflict of interest.

Copyright © 2013 Elsevier Inc. All rights reserved.

Figures

FIG. 1

Representative hematoxylin and eosin stained microscopic images from non-uremic (panel A) and uremic (panel B) subjects are shown. Uremic subjects showed increased interlobar fibrosis, increased microvascular density, increased vascular wall hypertrophy, scattered fat necrosis, and lower adipocyte volumes as compared to non-uremic subjects. Scale bar = 200 μm.

FIG. 2

Comparison of leptin, adiponectin and resistin levels between uremic and non-uremic patients.