Dietary supplementation with rice bran or navy bean alters gut bacterial metabolism in colorectal cancer survivors
Amy M Sheflin, Erica C Borresen, Jay S Kirkwood, Claudia M Boot, Alyssa K Whitney, Shen Lu, Regina J Brown, Corey D Broeckling, Elizabeth P Ryan, Tiffany L Weir, Amy M Sheflin, Erica C Borresen, Jay S Kirkwood, Claudia M Boot, Alyssa K Whitney, Shen Lu, Regina J Brown, Corey D Broeckling, Elizabeth P Ryan, Tiffany L Weir
Abstract
Scope: Heat-stabilized rice bran (SRB) and cooked navy bean powder (NBP) contain a variety of phytochemicals that are fermented by colonic microbiota and may influence intestinal health. Dietary interventions with these foods should be explored for modulating colorectal cancer risk.
Methods and results: A randomized-controlled pilot clinical trial investigated the effects of eating SRB (30 g/day) or cooked navy bean powder (35 g/day) on gut microbiota and metabolites (NCT01929122). Twenty-nine overweight/obese volunteers with a prior history of colorectal cancer consumed a study-provided meal and snack daily for 28 days. Volunteers receiving SRB or NBP showed increased gut bacterial diversity and altered gut microbial composition at 28 days compared to baseline. Supplementation with SRB or NBP increased total dietary fiber intake similarly, yet only rice bran intake led to a decreased Firmicutes:Bacteroidetes ratio and increased SCFA (propionate and acetate) in stool after 14 days but not at 28 days.
Conclusion: These findings support modulation of gut microbiota and fermentation byproducts by SRB and suggest that foods with similar ability to increase dietary fiber intake may not have equal effects on gut microbiota and microbial metabolism.
Keywords: Bile acids; Heat-stabilized rice bran; Navy bean; Short chain fatty acids; Stool microbiota.
Conflict of interest statement
Authors have no conflict of interest to declare for this research.
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Figures
![Figure 1](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/5209300/bin/nihms833860f1.jpg)
![Figure 2](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/5209300/bin/nihms833860f2.jpg)
Figure 3. Predicted differences in stool bacterial…
Figure 3. Predicted differences in stool bacterial functional categories (PICRUSt)
Only KEGG categories found significant…
Figure 4
Changes in stool short chain…
Figure 4
Changes in stool short chain fatty acids (SCFAs) shown in a) Day 14…
Figure 4
Changes in stool short chain…
Figure 4
Changes in stool short chain fatty acids (SCFAs) shown in a) Day 14…
- Pilot dietary intervention with heat-stabilized rice bran modulates stool microbiota and metabolites in healthy adults.Sheflin AM, Borresen EC, Wdowik MJ, Rao S, Brown RJ, Heuberger AL, Broeckling CD, Weir TL, Ryan EP. Sheflin AM, et al. Nutrients. 2015 Feb 16;7(2):1282-300. doi: 10.3390/nu7021282. Nutrients. 2015. PMID: 25690418 Free PMC article. Clinical Trial.
- Navy Beans Impact the Stool Metabolome and Metabolic Pathways for Colon Health in Cancer Survivors.Baxter BA, Oppel RC, Ryan EP. Baxter BA, et al. Nutrients. 2018 Dec 22;11(1):28. doi: 10.3390/nu11010028. Nutrients. 2018. PMID: 30583518 Free PMC article. Clinical Trial.
- Effects of a Rice Bran Dietary Intervention on the Composition of the Intestinal Microbiota of Adults with a High Risk of Colorectal Cancer: A Pilot Randomised-Controlled Trial.So WKW, Chan JYW, Law BMH, Choi KC, Ching JYL, Chan KL, Tang RSY, Chan CWH, Wu JCY, Tsui SKW. So WKW, et al. Nutrients. 2021 Feb 6;13(2):526. doi: 10.3390/nu13020526. Nutrients. 2021. PMID: 33561964 Free PMC article. Clinical Trial.
- Can the gastrointestinal microbiota be modulated by dietary fibre to treat obesity?Davis HC. Davis HC. Ir J Med Sci. 2018 May;187(2):393-402. doi: 10.1007/s11845-017-1686-9. Epub 2017 Oct 16. Ir J Med Sci. 2018. PMID: 29038988 Review.
- Current Hypothesis for the Relationship between Dietary Rice Bran Intake, the Intestinal Microbiota and Colorectal Cancer Prevention.So WK, Law BM, Law PT, Chan CW, Chair SY. So WK, et al. Nutrients. 2016 Sep 15;8(9):569. doi: 10.3390/nu8090569. Nutrients. 2016. PMID: 27649240 Free PMC article. Review.
- Composition, Microbiota, Mechanisms, and Anti-Obesity Properties of Rice Bran.Sivamaruthi BS, Alagarsamy K, Thangaleela S, Bharathi M, Kesika P, Chaiyasut C. Sivamaruthi BS, et al. Foods. 2023 Mar 18;12(6):1300. doi: 10.3390/foods12061300. Foods. 2023. PMID: 36981226 Free PMC article. Review.
- Randomized Controlled Trial
- Research Support, Non-U.S. Gov't
- Adult
- Aged
- Aged, 80 and over
- Colorectal Neoplasms / microbiology*
- Dietary Supplements*
- Feces / microbiology
- Female
- Gastrointestinal Microbiome / drug effects*
- Gastrointestinal Microbiome / genetics
- Humans
- Male
- Middle Aged
- Oryza*
- Survivors
- ClinicalTrials.gov/NCT01929122
- ClinicalTrials.gov/NCT01929122
- Full Text Sources
- Other Literature Sources
- Medical
NCBI Literature Resources
The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.
National Library of Medicine
8600 Rockville Pike
Bethesda, MD 20894
![Figure 3. Predicted differences in stool bacterial…](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/5209300/bin/nihms833860f3.jpg)
Figure 4
Changes in stool short chain…
Figure 4
Changes in stool short chain fatty acids (SCFAs) shown in a) Day 14…
Figure 4
Changes in stool short chain…
Figure 4
Changes in stool short chain fatty acids (SCFAs) shown in a) Day 14…
- Pilot dietary intervention with heat-stabilized rice bran modulates stool microbiota and metabolites in healthy adults.Sheflin AM, Borresen EC, Wdowik MJ, Rao S, Brown RJ, Heuberger AL, Broeckling CD, Weir TL, Ryan EP. Sheflin AM, et al. Nutrients. 2015 Feb 16;7(2):1282-300. doi: 10.3390/nu7021282. Nutrients. 2015. PMID: 25690418 Free PMC article. Clinical Trial.
- Navy Beans Impact the Stool Metabolome and Metabolic Pathways for Colon Health in Cancer Survivors.Baxter BA, Oppel RC, Ryan EP. Baxter BA, et al. Nutrients. 2018 Dec 22;11(1):28. doi: 10.3390/nu11010028. Nutrients. 2018. PMID: 30583518 Free PMC article. Clinical Trial.
- Effects of a Rice Bran Dietary Intervention on the Composition of the Intestinal Microbiota of Adults with a High Risk of Colorectal Cancer: A Pilot Randomised-Controlled Trial.So WKW, Chan JYW, Law BMH, Choi KC, Ching JYL, Chan KL, Tang RSY, Chan CWH, Wu JCY, Tsui SKW. So WKW, et al. Nutrients. 2021 Feb 6;13(2):526. doi: 10.3390/nu13020526. Nutrients. 2021. PMID: 33561964 Free PMC article. Clinical Trial.
- Can the gastrointestinal microbiota be modulated by dietary fibre to treat obesity?Davis HC. Davis HC. Ir J Med Sci. 2018 May;187(2):393-402. doi: 10.1007/s11845-017-1686-9. Epub 2017 Oct 16. Ir J Med Sci. 2018. PMID: 29038988 Review.
- Current Hypothesis for the Relationship between Dietary Rice Bran Intake, the Intestinal Microbiota and Colorectal Cancer Prevention.So WK, Law BM, Law PT, Chan CW, Chair SY. So WK, et al. Nutrients. 2016 Sep 15;8(9):569. doi: 10.3390/nu8090569. Nutrients. 2016. PMID: 27649240 Free PMC article. Review.
- Composition, Microbiota, Mechanisms, and Anti-Obesity Properties of Rice Bran.Sivamaruthi BS, Alagarsamy K, Thangaleela S, Bharathi M, Kesika P, Chaiyasut C. Sivamaruthi BS, et al. Foods. 2023 Mar 18;12(6):1300. doi: 10.3390/foods12061300. Foods. 2023. PMID: 36981226 Free PMC article. Review.
- Randomized Controlled Trial
- Research Support, Non-U.S. Gov't
- Adult
- Aged
- Aged, 80 and over
- Colorectal Neoplasms / microbiology*
- Dietary Supplements*
- Feces / microbiology
- Female
- Gastrointestinal Microbiome / drug effects*
- Gastrointestinal Microbiome / genetics
- Humans
- Male
- Middle Aged
- Oryza*
- Survivors
- ClinicalTrials.gov/NCT01929122
- ClinicalTrials.gov/NCT01929122
- Full Text Sources
- Other Literature Sources
- Medical
NCBI Literature Resources
The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.
National Library of Medicine
8600 Rockville Pike
Bethesda, MD 20894
![Figure 4](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/5209300/bin/nihms833860f4a.jpg)
Figure 4
Changes in stool short chain…
Figure 4
Changes in stool short chain fatty acids (SCFAs) shown in a) Day 14…
- Pilot dietary intervention with heat-stabilized rice bran modulates stool microbiota and metabolites in healthy adults.Sheflin AM, Borresen EC, Wdowik MJ, Rao S, Brown RJ, Heuberger AL, Broeckling CD, Weir TL, Ryan EP. Sheflin AM, et al. Nutrients. 2015 Feb 16;7(2):1282-300. doi: 10.3390/nu7021282. Nutrients. 2015. PMID: 25690418 Free PMC article. Clinical Trial.
- Navy Beans Impact the Stool Metabolome and Metabolic Pathways for Colon Health in Cancer Survivors.Baxter BA, Oppel RC, Ryan EP. Baxter BA, et al. Nutrients. 2018 Dec 22;11(1):28. doi: 10.3390/nu11010028. Nutrients. 2018. PMID: 30583518 Free PMC article. Clinical Trial.
- Effects of a Rice Bran Dietary Intervention on the Composition of the Intestinal Microbiota of Adults with a High Risk of Colorectal Cancer: A Pilot Randomised-Controlled Trial.So WKW, Chan JYW, Law BMH, Choi KC, Ching JYL, Chan KL, Tang RSY, Chan CWH, Wu JCY, Tsui SKW. So WKW, et al. Nutrients. 2021 Feb 6;13(2):526. doi: 10.3390/nu13020526. Nutrients. 2021. PMID: 33561964 Free PMC article. Clinical Trial.
- Can the gastrointestinal microbiota be modulated by dietary fibre to treat obesity?Davis HC. Davis HC. Ir J Med Sci. 2018 May;187(2):393-402. doi: 10.1007/s11845-017-1686-9. Epub 2017 Oct 16. Ir J Med Sci. 2018. PMID: 29038988 Review.
- Current Hypothesis for the Relationship between Dietary Rice Bran Intake, the Intestinal Microbiota and Colorectal Cancer Prevention.So WK, Law BM, Law PT, Chan CW, Chair SY. So WK, et al. Nutrients. 2016 Sep 15;8(9):569. doi: 10.3390/nu8090569. Nutrients. 2016. PMID: 27649240 Free PMC article. Review.
- Composition, Microbiota, Mechanisms, and Anti-Obesity Properties of Rice Bran.Sivamaruthi BS, Alagarsamy K, Thangaleela S, Bharathi M, Kesika P, Chaiyasut C. Sivamaruthi BS, et al. Foods. 2023 Mar 18;12(6):1300. doi: 10.3390/foods12061300. Foods. 2023. PMID: 36981226 Free PMC article. Review.
- Randomized Controlled Trial
- Research Support, Non-U.S. Gov't
- Adult
- Aged
- Aged, 80 and over
- Colorectal Neoplasms / microbiology*
- Dietary Supplements*
- Feces / microbiology
- Female
- Gastrointestinal Microbiome / drug effects*
- Gastrointestinal Microbiome / genetics
- Humans
- Male
- Middle Aged
- Oryza*
- Survivors
- ClinicalTrials.gov/NCT01929122
- ClinicalTrials.gov/NCT01929122
- Full Text Sources
- Other Literature Sources
- Medical
![Figure 4](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/5209300/bin/nihms833860f4b.jpg)
Source: PubMed