Platelet aggregation and quality control of platelet concentrates produced in the Amazon Blood Bank

Maria José Dantas Coêlho, Taysa de Castro Monteiro, Felicien Gonçalves Vasquez, Kátia Luz Torres Silva, Kleber Sandro Brasil Dos Santos, Viviana Maria Araújo de Oliveira, Francimary de Oliveira Cavalcante, Maria José Dantas Coêlho, Taysa de Castro Monteiro, Felicien Gonçalves Vasquez, Kátia Luz Torres Silva, Kleber Sandro Brasil Dos Santos, Viviana Maria Araújo de Oliveira, Francimary de Oliveira Cavalcante

Abstract

Background: The study of platelet aggregation is essential to assess in vitro platelet function by different platelet activation pathways.

Objective: To assess aggregation and biochemical parameters of random platelet concentrates produced at the Fundação HEMOAM using the quality control tests defined by law.

Methods: Whole blood samples from 80 donors and the respective platelet concentrate units were tested. Platelet concentrates were tested (platelet count, aggregation and pH) on days 1, 3 and 5 of storage. Additionally a leukocyte count was done only on day 1 and microbiological tests on day 5 of storage. Collagen and adenosine diphosphate were used as inducing agonists for platelet aggregation testing.

Results: Donor whole blood had normal aggregation (aggregation with adenosine diphosphate = 67% and with collagen = 78%). The median aggregation in platelet concentrates with adenosine diphosphate was low throughout storage (18% on day 1, 7% on day 3 and 6% on day 5) and the median aggregation with collagen was normal only on day 1 and low thereafter (54.4% on day 1, 20.5% on day 3 and 9% on day 5).

Conclusion: Although the results were within the norms required by law, platelet concentrates had low aggregation rates. We suggest the inclusion of a functional assessment test for the quality control of platelet concentrates for a more effective response to platelet replacement therapy.

Keywords: Blood banks; Blood component transfusion; Collagen; Hemostasis; Platelet aggregation; Quality control.

Conflict of interest statement

Conflict-of-interest disclosure: The authors declare no competing financial interest

References

    1. Thon JN, Italiano JE.Platelet formation. Semin Hematol. 2010; 47(3): 220-6
    1. Andrews RK, Gardiner EE, Shen Y, Berndt MC.Platelet interactions in thrombosis. IUBMB Life. 2004; 56(1): 13-8
    1. Schulze H, Shivdasani RA.Mechanisms of thrombopoiesis. J Thromb Haemost. 2005; 3(8): 1717-24
    1. Erhardtsen general haemostasis: the evidence-based route. Pathophysiol Haemost Thromb. 2002; 32Suppl 1: 47-52
    1. Flaumenharft R.Molecular basis of platelet granule secretion. Arteroscler Thromb Vasc Biol. 2003; 23(7): 1152-60
    1. Reed GL.Platelet secretory mechanisms. Semin Thromb Haemost. 2004; 30(4): 441-50
    1. Castro HC, Ferreira BL, Nagashima T, Schueler A, Rueff C, Camisasca D, et al. Plaquetas: ainda um alvo terapêutico. J Bras Patol Med Lab. 2006; 42(5): 321-32
    1. Favaloro EJ.Internal quality control and external quality assurance of platelet function tests. Semin Thromb Hemost. 2009; 35 (2): 139-49
    1. Cardigan R, Turner C, Harrison P.Current methods of assessing platelet function: relevance to transfusion medicine. Vox Sang. 2005; 88(3): 153-63
    1. Tynngård N.Preparation, storage and quality control of platelet concentrates. Transfus Apher Sci. 2009; 41(2): 97-104
    1. Seghatchian J, Krailadsiri P.Current position on preparation and quality of leucodepleted platelet concentrates for clinical use. Transfus Sci. 2000; 22(1-2): 85-8
    1. Born GV, Cross MJ.The aggregation of blood platelets. J Physiol. 1963; 168: 178-95
    1. Neiva TJ, Brocado G, Ferreira JS, Ferreira VL, Castro CE.Avaliaçäo plaquetária de doadores de sangue. Rev Bras Anal Clin. 2000; 32 (4): 249-52
    1. Tostes MA, Tostes Jr S, Pereira GA, Soares S, Moraes-Souza H.Influência da coleta, da produção e da estocagem na qualidade dos concentrados de plaquetas. Rev Bras Hematol Hemoter. 2008; 30 (5): 367-73
    1. Brasil. Ministério da Saúde Resolução RDC nº 153, de 14 de junho de 2004. Determina o Regulamento Técnico para os procedimentos hemoterápicos, incluíndo a coleta, o processamento, a testagem, o armazenamento, o transporte, o controle de qualidade e o uso humano de sangue, e seus componentes, obtidos do sangue venoso, do cordão umbilical, da placenta e da medula óssea [Internet] [cited 2011 Apr 5] Available from:
    1. Miyaji R, Sakai M, Urano H, Nakata K, Sakamoto H, Shirahata A.Decreased platelet aggregation of platelet concentrate during storage recovers in the body transfusion. Transfusion. 2004; 44 (6): 891-9
    1. Di Minno G, Mannucci PM, Tufano A, Palareti G, Moia M, Baccaglini U, et al. The first ambulatory screening on thromboembolism: a multicentre, cross-sectional, observational study on risk factors for venous thromboembolism. J Thromb Haemost. 2005; 3(7): 1459-66
    1. Neiva TJ, Machado MJ, Hoehn M, Hermes EM, Vituri CL, Ferreira JS, et al. Evaluation of platelet aggregation in platelet concentrates (PCs): storage implications. Rev Bras Hematol Hemoter. 2003; 25(4) 207-12; Erratum in Rev Bras Hematol Hemoter. 2004; 1(26):68
    1. Shenkman B, Einav Y, Salomon O, Varon D, Savion N.Testing agonist-induced platelet aggregation by the Impact-R [Cone and plate(let) analyzer (CPA)]. Platelets. 2008; 19(6): 440-6 Erratum in: Platelets. 2008;19(8):641
    1. Jilma-Stohlawetz P, Horvath M, Eichelberger B, Koren D, Jilma B, Panzer S.Platelet function under high-shear conditions from platelet concentrates. Transfusion. 2008; 48(1): 129-35
    1. Cardigan R, Williamson LM.The quality of platelets after storage for 7 days. Transfus Med. 2003; 13(4): 173-87
    1. Bowbrick VA, Mikhailidis DP, Stansby G.Influence of platelet count and activity on thromboelastography parameters. Platelets. 2003; 14(4): 219-24
    1. Henschler R, Muller MM, Pfeiffer HU, Seifried E, Sireis W.Production of standard blood components. ISBT Science Series. 2010; 5(1): 190-5
    1. Dekkers DW, De Cuyper IM, van der Meer PF, Verhoeven AJ, de Korte D.Influence of pH on stored human platelets. Transfusion. 2007; 47(10): 1889-95
    1. Baker JM, Candy DJ, Hawker RJ.Influences of pH on human platelet metabolism. Platelets. 2001; 12(6): 333-42
    1. Mathai J.Problem of bacterial contamination in platelet concentrates. Transfus Apher Sci. 2009; 41(2): 139-44
    1. Walther-Wenke G.Incidence of bacterial transmission and transfusion reactions by blood components. Clin Chem Lab Med. 2008; 46(7): 919-25

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