Micromorphology and microhardness of enamel after treatment with home-use bleaching agents containing 10% carbamide peroxide and 7.5% hydrogen peroxide

Robson Tetsuo Sasaki, Alex José Arcanjo, Flávia Martão Flório, Roberta Tarkany Basting, Robson Tetsuo Sasaki, Alex José Arcanjo, Flávia Martão Flório, Roberta Tarkany Basting

Abstract

Objective: The purpose of this study was to assess the effect of home-use bleaching agents containing 10% carbamide peroxide and 7.5% hydrogen peroxide on enamel microhardness and surface micromorphology.

Material and methods: Enamel slabs (n=10) received the bleaching agents for 1 h/day and remained in artificial saliva solution for 23 h/day, during a total period of 21 days. Control group was composed of enamel slabs that were not subjected to treatment with the agents and were maintained in artificial saliva solution. Microhardness tests were performed before treatment application, 21 days of treatment and 14 days after the end of treatment. Scanning electron microscopy analyses were performed after 14 days after the end of bleaching treatment by 3 calibrated observers who attributed scores.

Results: The Tukey's test (alpha=0.05) showed no significant differences in microhardness values among bleaching agents, at 21 days of treatment and a significant increase in microhardness for different agents after 14 days from the end of treatment. Fisher's exact test showed differences in micromorphology of enamel between control and experimental groups (p=0.0342).

Conclusions: Bleaching agents containing 10% carbamide peroxide and 7.5% hydrogen peroxide may change surface micromorphology of enamel, although no changes in microhardness were observed.

Figures

FIGURE 1. Basic composition and manufacturer of…
FIGURE 1. Basic composition and manufacturer of each bleaching agent
FIGURE 2. Scores attributed by the examiners,…
FIGURE 2. Scores attributed by the examiners, according to the type of surface
FIGURE 3. SEM micrograph of enamel surface…
FIGURE 3. SEM micrograph of enamel surface subjected to immersion in artificial saliva (control group), presenting no erosions or porosities (x1,500)
FIGURE 4. SEM micrograph of enamel exposed…
FIGURE 4. SEM micrograph of enamel exposed to a bleaching agent containing 7.5% hydrogen peroxide (Day White 2Z), presenting erosions (x1,500)
FIGURE 5. SEM micrograph of enamel subjected…
FIGURE 5. SEM micrograph of enamel subjected to bleaching agent containing 10% carbamide peroxide (Platinum), presenting erosions (x1,500)

References

    1. Arends J, Jongebloed WL, Goldberg M, Schuthof J. Interaction of urea and human enamel. Caries Res. 1997;26:531–539.
    1. Attin T, Kielbassa AM, Schawanenberg M, Helliwig E. The effect of fluoride treatment on remineralization of bleached enamel. J Oral Rehabil. 1997;24(4):282–286.
    1. Attin T, Vollmer D, Wiegand A, Attin R, Betke H. Subsurface microhardness of enamel and dentin after different external bleaching procedures. Am J Dent. 2005;18(1):8–12.
    1. Basting RT, Rodrigues AL, Serra MC. Micromorphology and surface roughness of sound and demineralized enamel and dentin bleached with a 10% carbamide peroxide bleaching agent. Am J Dent. 2007;20(2):97–102.
    1. Basting RT, Rodrigues AL, Jr, Serra MC. The effect of 10% carbamide peroxide bleaching material on microhardness of sound and demineralized enamel and dentin in situ. Oper Dent. 2001;26(6):531–539.
    1. Basting RT, Rodrigues AL, Jr, Serra MC. The effect of 10% carbamide peroxide, carbopol and/or glycerin on enamel and dentin microhardness. Oper Dent. 2005;30(5):608–616.
    1. Bitter NC. A scanning electron microscope study of the long-term effect of bleaching agents on the enamel surface in vivo. Gen Dent. 1998;46(1):84–88.
    1. Chen HP, Chang CH, Liu JK, Chuang SF, Yang JY. Effect of fluoride containing bleaching agents on enamel surface properties. J Dent. 2008;36(9):718–725.
    1. Featherstone JD, O'Really MM, Shariati M, Brugler S. Factors relating to demineralization and remineralization of the teeth. In: Leach AS, editor. Enhancement of remineralization in vitro and in vivo. Oxford: IRL; 1986. pp. 23–34.
    1. Featherstone JD, ten Cate JM, Shariati M, Arends J. Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles. Caries Res. 1983;17(5):385–391.
    1. Freitas PM, Basting RT, Rodrigues AL, Jr, Serra MC. Effects of two 10% peroxide carbamide bleaching agents on dentin microhardness at different time intervals. Quintessence Int. 2002;33(5):370–375.
    1. Fu B, Hoth-Hannig W, Hannig M. Effects of dental bleaching on micro- and nano-morphological alterations of the enamel surface. Am J Dent. 2007;20(1):35–40.
    1. Goldberg M, Arends J, Jonegbloed WL, Schuthof J, Septier D. Action of urea solutions on human enamel surfaces. Caries Res. 1983;17(2):106–112.
    1. Goldstein GR, Garber DA. Complete dental bleaching. Chicago: Quintessence Publishing; 1995.
    1. Gurgan S, Bolay S, Alacam R. In vitro adherence of bacteria to bleached or unbleached enamel surfaces. J Oral Rehabil. 1997;24(8):624–627.
    1. Haywood VB, Heymann HO. Nightguard vital bleaching. Quintessence Int. 1989;20(3):173–176.
    1. Hegedüs C, Bistey T, Flora-Nagy E, Keszthelyi G, Jenei A. An atomic force microscopy study on the effect of bleaching agents on enamel surface. J Dent. 1999;27(7):509–515.
    1. Landis J, Koch GG. The measurement of observer agreement for categorical data. Biometrics. 1977;33(1):159–174.
    1. Lee CQ, Cobb CM, Zargartalebi F, Hu N. Effect of bleaching on microhardness, morphology and color of enamel. Gen Dent. 1995;43(2):158–162.
    1. Markovic L, Jordan RA, Lakota N, Gaengler P. Micromorphology of enamel surface after vital tooth bleaching. J Endod. 2007;33(5):607–610.
    1. Marshall MV, Gragg PP, Packman EW, Wright PB, Cancro LP. Hydrogen peroxide decomposition in the oral cavity. Am J Dent. 2001;14(1):39–45.
    1. McCracken MS, Haywood VB. Demineralization effects of 10 percent carbamide peroxide. J Dent. 1996;24(6):395–398.
    1. Mokllis GR, Matis BA, Cochran MA, Eckert GJ. A clinical evaluation of carbamide peroxide and hydrogen peroxide whitening agents during daytime use. J Am Dent Assoc. 2000;131(9):1269–1277.
    1. Oliveira R, Basting RT, Rodrigues JA, Rodrigues AL, Jr, Serra MC. Effects of a carbamide peroxide agent and desensitizing dentifrices on enamel microhardness. Am J Dent. 2001;16(1):42–46.
    1. Rodrigues JA, Basting RT, Serra MC, Rodrigues LA., Jr Effects of 10% carbamide peroxide bleaching materials on enamel microhardness at different time intervals. Am J Dent. 2001;14(2):67–71.
    1. Serra MC, Cury JA. The in vitro effect of glass-ionomer cement restoration on enamel subjected to a demineralization and remineralization model. Quintessence Int. 1992;23(2):143–147.
    1. Smidt A, Weller D, Roman I, Gedalia I. Effect of bleaching agents on microhardness and surface morphology of tooth enamel. Am J Dent. 1998;11(2):83–85.
    1. Wattanapayungkul P, Matis BA, Cochran MA, Moore BK. A clinical study of the effect of pellicle on the degradation of 10% carbamide peroxide within the first hour. Quintessence Int. 1999;30(11):737–741.

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