In Vivo Culture System Using the INVOcell Device Shows Similar Pregnancy and Implantation Rates to Those Obtained from In Vivo Culture System in ICSI Procedures

Javier García-Ferreyra, Roly Hilario, Daniel Luna, Lucy Villegas, Rocío Romero, Patricia Zavala, Julio Dueñas-Chacón, Javier García-Ferreyra, Roly Hilario, Daniel Luna, Lucy Villegas, Rocío Romero, Patricia Zavala, Julio Dueñas-Chacón

Abstract

Capsule: Clinical outcomes using INVOcell device with ICSI.

Objective: Intravaginal culture of oocytes (INVO) procedure is an intravaginal culture system that utilizes the INVOcell device in which the fertilization and embryo culture occur. In this procedure, the vaginal cavity serves as an incubator for oocyte fertilization and early embryonic development. The objective of this study was to evaluate the clinical outcomes of this intravaginal culture system in intracytoplasmic sperm injection (ICSI).

Methods: A total of 24 cycles INVO-ICSI (study group) and 74 cycles of ICSI (control group) were included in the study. The cleaved oocytes at day 3/total injected oocytes, embryo quality, pregnancy rate (PR), implantation rate (IR), and miscarriage rate (MR) were compared between both groups.

Results: At day 3, there was no difference in the cleaved oocyte rate (78.7 and 76.1%) and embryo quality (77 and 86.8%) for the study and control groups, respectively. In the study group, more embryos were significantly transferred compared to the control group (2.63 ± 0.58 versus 1.93 ± 0.25; P < 0.05). PRs, IRs, and MRs were similar for the study group compared with the control group (PR: 54.2% versus 58.1%; IR: 31.7% versus 33.6%; MR: 7.7% versus 20.9%).

Conclusions: Good PR and IR can be obtained using the INVOcell device, and the INVO-ICSI procedure can be considered as an alternative option to infertile patients.

Keywords: ICSI; INVO; INVOcell; implantation rate; pregnancy rate.

Figures

Figure 1
Figure 1
Inner chamber and outer rigid shell of the INVOcell device.
Figure 2
Figure 2
PRs, IRs, and MRs.

References

    1. Hansen M, Boer C, Mine E, de Klerk N, Kurinczuck JJ. Assisted reproductive techonologies and the risk of birth defects—a systematic review. Hum Reprod. 2005;20:328–338.
    1. Ranoux C, Aubriot FX, Dubuisson JB, et al. A new in vitro fertilization technique: intravaginal culture. Fertil Steril. 1988;49:654–657.
    1. Fukuda M, Fukuda K, Ranoux C. Unexpected low oxygen tension of intravaginal culture. Hum Reprod. 1996;11:1293–1295.
    1. Frydman R, Ranoux C. INVO: a simple, low cost effective assisted reproductive technology. ESHRE monographs, special task force on “developing countries and infertility”. Hum Reprod. 2008;163:85–89.
    1. Ranoux C, Seibel MM. New techniques in fertilization: intravaginal culture and microvolume straw. J In Vitro Fert Embryo Transf. 1990;7:6–8.
    1. Bonaventura L, Ahlering P, Morris R, Mouchel J, Scheiber M, Batzofin J. The INVOcell, a new medical device for intra vaginal fertilization and culture. Fertil Steril. 2006;86(3):S164.
    1. Lucena E, Saa AM, Navarro DE, Pulido C, Lombana O, Moran A. INVO procedure: minimally invasive IVF as an alternative treatment option for infertile couples. Sci World J. 2012;2:1–6.
    1. Ranoux C. In vivo embryo culture device. In: Nagy ZP, Varghese A, Agarwal A, editors. Practical Manual of In Vitro Fertilization. Berlin: Springer; 2012. pp. 161–169.
    1. Palermo GD, Neri QV, Monahan D, Kocent J, Rosenwaks Z. Development and current applications of assisted fertilization. Fertil Steril. 2012;97:248–259.
    1. Tavmergen E, Goker E, Sendag F, Levi R. Comparison of short and long ovulation induction protocols used in ART applications according to the ovarian response and outcome of pregnancy. Arch Gynecol Obstet. 2002;266:5–11.
    1. World Health Organization . Laboratory Manual for Examination and Processing. 5th ed. Geneva: WHO; 2010.
    1. García J, Noriega Hoces L, Gonzales GF. Sperm chromatin stability and its relationship with fertilization rate after intracytoplasmic sperm injection (ICSI) in an assisted reproduction program. J Assist Reprod Genet. 2007;24:587–593.
    1. Gardner DK, Schoolcraft WB. In vitro culture of human blastocysts. In: Jansen R, Mortimer D, editors. Towards Reproductive Certainly: Infertility and Genetics Beyond. UK: Parthenon press; 1999. p. 378.
    1. Mansour R. Minimizing embryo expulsion after embryo transfer: a randomized controlled study. Hum Reprod. 2005;20:170–174.
    1. Abdelmassih V, Balmaceda JP, Nagy ZP, Abdelmassih S, Abdelmassih R. ICSI and day 5 embryo transfers: higher implantation rates and lower rate of multiple pregnancy with prolonged culture. Reprod Biomed Online. 2001;3:216–220.
    1. Karaki RZ, Samarraie SS, Younis NA, Lahloub TM, Ibrahim MH. Blastocsyt culture and transfer: a step toward improved in vitro fertilization outcome. Fertil Steril. 2002;77:114–118.
    1. Gardner D, Lane M. Embryo culture in textbook of assisted reproductive techniques: laboratory and clinical perspective. In: Gardner D, Weissman A, Howles C, Shoham Z, editors. Laboratory and Clinical Perspectives. London: Martin Dunitz Press; 2001. pp. 203–222.
    1. Burton GJ, Hempstock J, Jauniaux E. Oxygen, early embryonic metabolism and free radical-mediated embryopathies. Reprod Biomed Online. 2000;6:84–96.
    1. Bavister BD. The role of animal studies in supporting human ART. Reprod Fert Dev. 2004;16:719–728.
    1. Karagenc L, Sertkaya Z, Ciray N. Impact of oxygen concentration on embryonic development of mouse zygotes. Reprod Biomed Online. 2004;9:409–417.
    1. Sepúlveda S, García J, Arriaga E, Díaz J, Noriega-Portella L, Noriega-Hoces L. In vitro development and pregnancy outcomes for human embryos cultured in either a single medium or in a sequential media system. Fertil Steril. 2008;91:1765–1770.
    1. Reed ML, Hamic A, Thompson DJ, Caperton CL. Continuous uninterrupted single medium culture without medium renewal versus sequential media culture: a sibling embryo study. Fertil Steril. 2009;92:1783–1786.
    1. Costa-Borges N, Belles M, Herreros J, et al. Single medium culture in a time-lapse incubator until the blastocyst stage with or without medium renewal on Day-3: a prospective randomized study with donor oocytes. Hum Reprod. 2013;28(suppl 1):167.
    1. Vieira GH, Colucci FA. Comparative preliminary study between the conventional IVF/ICSI and the INVO intra-vaginal device: stress-related psychological impact. JBRA Assist Reprod. 2013;17:300–303.

Source: PubMed

Sponsors en medewerkers

Medische omstandigheden

Geneesmiddelinterventies

3
Abonneren