A selective cyclooxygenase-2 inhibitor suppresses tumor growth in nude mouse xenografted with human head and neck squamous carcinoma cells

G Nishimura, S Yanoma, H Mizuno, K Kawakami, M Tsukuda, G Nishimura, S Yanoma, H Mizuno, K Kawakami, M Tsukuda

Abstract

The anti-tumor effect of a selective cyclooxygenase (COX)-2 inhibitor, JTE-522, was examined with the human head and neck squamous cell carcinoma cell line KB. KB cells do not produce prostaglandin (PG)-E2. In vitro, JTE-522 induced an increase of G1 phase-arrested cells, suppression of platelet-derived growth factor (PDGF) production and inhibition of telomerase activity. No cytotoxic effect was detected. In vivo, the growth of the tumor xenografted into nude mice was significantly suppressed by JTE-522. Suppression of angiogenesis at the periphery of the tumor, increase of G1-arrested cells and suppression of telomerase activity were observed, together with an increase of apoptotic cell death in the tumor. Immunological enhancement did not play a role. We concluded that the anti-tumor effect of JTE-522 was caused by anti-angiogenesis action, cell cycle arrest and inhibition of telomerase activity of the tumor cells. These combined effects might induce apoptosis.

References

    1. Kelloff , G. J. , Boone , C. W. , Crowell , J. A. , Steele , V. E. , Lubet , R. and Sigman , C. C.Chemopreventive drug development: perspectives and progress. Cancer Epidemiol . Biomarkers Prev. , 59 , 85 , 98 ( 1994. ).
    1. Giardiello , F. M. , Offerhaus , G. J. A. and DuBois , R. N.The role of nonsteroidal anti‐inflammatory drugs in colorectal cancer prevention . Eur. J. Cancer , 31A, 1071 , 1076 ( 1995. ).
    1. Moon , R. C. , Detrisac , C. J. , Thomas , C. F. and Kelloff , G. J.Chemoprevention of experimental bladder cancer . J. Cell. Biochem. , 161 ( Suppl. ), 134 – 138 ( 1992. ).
    1. Denda , A. , Endoh , T. , Kitajima , W. , Tang , Q. , Noguchi , O. , Kobayashi , Y. , Akai , H. , Okajima , E. , Tsujiuchi , T. , Tsutsumi , M. , Nakae , D. and Konishi , Y.Inhibition by piroxicam of oxidative DNA damage, liver cirrhosis and development of enzyme‐altered nodules caused by a cholinedeficient, L‐amino acid‐defined diet in rats . Carcinogenesis , 18 , 1921 – 1930 ( 1997. )
    1. Marnett , L. J.Aspirin and the potential role of prostaglandins in colon cancer . Cancer Res. , 59 , 5575 , 5589 ( 1992. ).
    1. Lupulescu , A.Prostaglandins, their inhibitors and cancer. Prostaglandins Leukotrienes Essent . Fatty Acids , 59 , 83 , 94 ( 1996. ).
    1. Eberhart , C. E. and DuBois , R. N.Eicosanoids and the gastrointestinal tract . Gastroenterology , 109 , 285 – 301 ( 1995. )
    1. Vane , J.Towards a better aspirin . Nature , 367 , 215 – 216 ( 1994. )
    1. Williams , C. S. , Smalley , W. and DuBois , R. N.Aspirin use and potential mechanisms for colorectal cancer prevention . J. Clin. Invest. , 59 , 1325 , 1329 ( 1997. ).
    1. Kargman , S. L. , O'Neill , G. P. , Vickers , P. J. , Evans , J. F. , Mancini , J. A. and Jothy , S.Expression of prostaglandin G/H synthase‐1 and ‐2 protein in human colon cancer . Cancer Res. , 59 , 2556 , 2559 ( 1995. ).
    1. DuBois , R. N. , Radhika , A. , Reddy , B. S. and Entingh , A. J.Increased cyclooxygenase‐2 levels in carcinogen‐induced rat colonic tumors . Gastroenterology , 59 , 1259 , 1262 ( 1996. ).
    1. Restimäki , A. , Honkanen , N. , Jänkälä , H. , Sipponen , P. and Härkönen , M.Expression of cyclooxygenase‐2 in human gastric carcinoma . Cancer Res. , 59 , 1276 , 1280 ( 1997. ).
    1. Müller‐Decker , K. , Scholz , K. , Marks , F. and Fürstenberger , G.Differential expression of prostaglandin H synthase isozymes during multistage carcinogenesis in mouse epidermis . Mol. Carcinog. , 59 , 31 , 41 ( 1995. ).
    1. Liu , X.‐H. and Rose , D. P.Differential expression and regulation of cyclooxygenase‐1 and ‐2 in two human breast cancer cell lines . Cancer Res. , 59 , 5125 , 5127 ( 1996. ).
    1. Tsujii , M. and DuBois , R. N.Alterations in cellular adhesion and apoptosis in epithelial cells overexpressing prostaglandin endoperoxide synthase 2 . Cell , 59 , 493 , 501 ( 1995. ).
    1. Oshima , M. , Dinchuk , J. E. , Kargman , S. L. , Oshima , H. , Hancock , B. , Kwong , E. , Trzaskos , J. M. , Evans , J. F. and Taketo , M. M.Suppression of intestinal polyposis in ApcD716 knockout mice by inhibition of cyclooxygenase 2 (COX‐2) . Cell , 59 , 803 , 809 ( 1996. ).
    1. Shiff , S. J. , Qiao , L. , Tsai , L. L. and Rigas , B.Sulindac sulfide, an aspirin‐like compound, inhibits proliferation, causes cell cycle quiescence, and induces apoptosis in HT‐ 29 colon adenocarcinoma cells . J. Clin. Invest. , 59 , 491 , 503 ( 1995. ).
    1. Duggan , D. E. , Hare , L. E. , Ditzler , C. A. , Lei , B. W. and Kwan , K. C.The disposition of sulindac . Clin. Pharmacol. Ther. , 59 , 326 , 335 ( 1977. ).
    1. Sheng , H. M. , Shao , J. Y. , Morrow , J. D. , Beauchamp , R. D. and DuBois , R. N.Modulation of apoptosis and bcl‐2 expression by prostaglandin E2 in human colon cancer cells . Cancer Res. , 59 , 362 , 366 ( 1998. ).
    1. Liu , X.‐H. , Yao , S. , Kirschenbaum , A. and Levine , A. C.NS398, a selective cyclooxygenase‐2 inhibitor, induces apoptosis and downregulates bcl‐2 expression in LNCaP cells . Cancer Res. , 59 , 4245 , 4249 ( 1998. ).
    1. Thompson , H. J. , Briggs , S. , Paranka , N. S. , Piazza , G. A. , Brendel , K. , Gross , P. H. , Sperl , G. J. , Pamukcu , R. and Ahnen , D. J.Inhibition of mammary carcinogenesis in rats by sulfone metabolite of sulindac . J. Natl. Cancer Inst. , 59 , 1259 , 1260 ( 1995. ).
    1. Tsujii , M. , Kawano , S. , Tsuji , S. , Sawaoka , H. , Hori , M. and DuBois , R. N.Cyclooxygenase regulates angiogenesis induced by colon cancer cell . Cell , 59 , 705 , 716 ( 1998. ).
    1. Eagle , H.Propagation in a fluid medium of a human epidermoid carcinoma, strain KB . Proc. Soc. Exp. Biol. Med. , 59 , 362 , 364 ( 1955. ).
    1. Matsushita , M. , Masaki , Y. , Yagi , Y. , Tanaka , T. and Wakitani , K.Pharmacological profile of JTE‐522, a novel prostaglandin H synthase‐2 inhibitor, in rats . Inflamm. Res. , 59 , 461 , 466 ( 1997. ).
    1. Mosmann , T.Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays . J. Immunol. Methods , 59 , 55 , 63 ( 1983. ).
    1. Kim , N. W. , Piatyszek , M. A. , Prowse , K. R. , Harley , C. B. , West , M. D. , Ho , P. L. C. , Coviello , G. M. , Wright , W. E. , Weinrich , S. L. and Shay , J. W.Specific association of human telomerase activity with immortal cells and cancer . Science , 59 , 2011 , 2014 ( 1994. ).
    1. Zhou , L.X. Biological behavior of hypopharyngeal carcinoma . J. Otolaryngol. Jpn. , 100 , 59 – 67 ( 1997. )
    1. Metzger , U. , Meier , J. , Uhlschmidt , G. and Weihe , H.Influence of various prostaglandin synthesis inhibitors on DNH‐induced rat colon cancer . Dis. Colon Rectum , 59 , 366 , 369 ( 1984. ).
    1. Narisawa , T. , Sato , M. , Tani , M. , Kudo , T. , Takahashi , T. and Goto , A.Inhibition of development of methylnitrosourea‐ induced rat colon tumors by indomethacin treatment . Cancer Res. , 59 , 1954 , 1957 ( 1981. ).
    1. Polland , M. and Luckert , P. H.Indomethacin treatment of rats with dimethylhydrazine‐induced intestinal tumors . Cancer Treat. Rev. , 59 , 1323 , 1327 ( 1980. ).
    1. Reddy , B. S. , Murayama , H. and Kelloff , G.Dose‐related inhibition of colon carcinogenesis by dietary piroxicam, a nonsteroidal anti‐inflammatory drug, during different stages of rat colon tumor development . Cancer Res. , 59 , 5340 , 5346 ( 1987. ).
    1. Rosenberg , L. , Palmer , J. R. , Zauber , A. G. , Warshauer , M. E. , Stolley , P. D. and Shapiro , S.A hypothesis: nonsteroidal anti‐inflammatory drugs reduce the incidence of largebowel cancer . J. Natl. Cancer Inst. , 59 , 355 , 358 ( 1991. ).
    1. Thun , M. J. , Namboodiri , M. M. and Heath , C. W. J.Aspirin use and reduced risk of fatal colon cancer . N. Engl. J. Med. , 59 , 1593 , 1596 ( 1991. ).
    1. Nugent , K. P. , Farmer , K. C. , Spiegelman , A. D. , Williams , C. B. and Phillips , R. K.Randomized double blind placebo controlled clinical trial of the effect of sulindac on duodenal and rectal polyposis and cell proliferation in familial adenomatous polyposis . Br. J. Surg. , 59 , 1618 , 1619 ( 1993. ).
    1. Sheng , H. , Shao , J. , Kirkland , S. C. , Isakson , P. , Coffey , R. , Morrow , J. , Beauchamp , R. D. and DuBois , R. N.Inhibition of human colon cancer cell growth by selective inhibition of cyclooxygenase‐2 . J. Clin. Invest. , 59 , 2254 , 2259 ( 1997. ).
    1. Kawamori , T. , Rao , C. V. , Seibert , K. and Reddy , B. S.Chemopreventive activity of celecoxib, a specific cyclooxygenase‐ 2 inhibitor, against colon carcinogenesis . Cancer Res. , 58 , 409 – 412 ( 1998. )
    1. Simmons , D. L. , Levy , D. B. , Yannoni , Y. and Erikson , R. L.Identification of a phorbol ester‐repressible v‐srcinducible gene . Proc. Natl. Acad. Sci. USA , 59 , 1178 , 1182 ( 1989. ).
    1. Hughes‐Fulford , M. , Appel , R. , Kumegawa , M. and Schmidt , J.Effect of dexamethasone on proliferating osteoblasts: inhibition of prostaglandin E2 synthesis, DNA synthesis, and alterations in actin cytoskeleton . Exp. Cell Res. , 59 , 150 , 156 ( 1992. ).
    1. Tjandrawinata , R. R. , Dahiya , R. and Hughes‐Fulford , M.Induction of cyclooxygenase‐2 mRNA by prostaglandin E2 in human prostatic carcinoma cells . Br. J. Cancer , 75 , 1111 , 1118 ( 1997. ).
    1. Levy , G. N.Prostaglandin H synthases, nonsteroidal antiinflammatory drugs, and colon cancer . FASEB J. , 11 , 234 , 247 ( 1997. ).
    1. Chinery , R. , Coffey , R. J. , Graves‐Deal , R. , Kirkland , S. C. , Sanchez , S. C. , Zackert , W. E. , Oates , J. A. and Morrow , J. D.Prostaglandin J2 and 15‐deoxy‐ Δ12,14‐prostaglandin J2 induce proliferation of cyclooxygenase‐dependent colorectal cancer cells . Cancer Res. , 59 , 2739 , 2746 ( 1999. ).
    1. Zimmermann , K. C. , Sarbia , M. , Weber , A.‐A. , Borchard , F. , Gabbert , H. E. and Schror , K.Cyclooxygenase‐2 expression in human esophageal carcinoma . Cancer Res. , 59 , 198 , 204 ( 1999. ).
    1. Kido , A. , Tsujiuchi , T. , Morishita , T. , Tsutsumi , M. , Takahama , M. , Miyauchi , Y. , Mii , Y. , Tamai , S. and Konishi , Telomerase Y.activity correlates with growth of transplantable osteosarcomas in rats treated with cis‐diammine dichloroplatinum or the angiogenesis inhibitor AGM‐ 1470 . Jpn. J. Cancer Res. , 59 , 1074 , 1081 ( 1998. ).

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