Carbohydrate-rich breakfast attenuates glycaemic, insulinaemic and ghrelin response to ad libitum lunch relative to morning fasting in lean adults

Enhad A Chowdhury, Judith D Richardson, Kostas Tsintzas, Dylan Thompson, James A Betts, Enhad A Chowdhury, Judith D Richardson, Kostas Tsintzas, Dylan Thompson, James A Betts

Abstract

Breakfast omission is associated with obesity and CVD/diabetes, but the acute effects of extended morning fasting upon subsequent energy intake and metabolic/hormonal responses have received less attention. In a randomised cross-over design, thirty-five lean men (n 14) and women (n 21) extended their overnight fast or ingested a typical carbohydrate-rich breakfast in quantities relative to RMR (i.e. 1963 (sd 238) kJ), before an ad libitum lunch 3 h later. Blood samples were obtained hourly throughout the day until 3 h post-lunch, with subjective appetite measures assessed. Lunch intake was greater following extended fasting (640 (sd 1042) kJ, P< 0.01) but incompletely compensated for the omitted breakfast, with total intake lower than the breakfast trial (3887 (sd 1326) v. 5213 (sd 1590) kJ, P< 0.001). Systemic concentrations of peptide tyrosine-tyrosine and leptin were greater during the afternoon following breakfast (both P< 0.05) but neither acylated/total ghrelin concentrations were suppressed by the ad libitum lunch in the breakfast trial, remaining greater than the morning fasting trial throughout the afternoon (all P< 0.05). Insulin concentrations were greater during the afternoon in the morning fasting trial (all P< 0.01). There were no differences between trials in subjective appetite during the afternoon. In conclusion, morning fasting caused incomplete energy compensation at an ad libitum lunch. Breakfast increased some anorectic hormones during the afternoon but paradoxically abolished ghrelin suppression by the second meal. Extending morning fasting until lunch altered subsequent metabolic and hormonal responses but without greater appetite during the afternoon. The present study clarifies the impact of acute breakfast omission and adds novel insights into second-meal metabolism.

Keywords: Appetite hormones; Breakfast skipping; Energy intake; Insulin sensitivity; Second-meal effect.

Figures

Fig. 1
Fig. 1
Energy intake during trials. In the morning fasting trial, an asymmetric normalised CI is plotted, the negative portion of which reflects the comparison between lunches and the positive portion reflects the comparison against total intake (i.e. lunch plus breakfast). An asterisk above a bar represents the comparison between the sum of the components of the bar, an asterisk between the bars represents the comparison between the specific component (P< 0·01). n 34, as one individual felt nauseous prior to lunch provision on one visit.
Fig. 2
Fig. 2
Metabolic responses during trials. (a) Plasma glucose (n 32), (b) serum insulin (n 32), (c) plasma NEFA (n 31), where missing data are due to insufficient blood for analysis. Values are means with their normalised CI represented by vertical bars. * Mean value was significantly different from the corresponding time point in other trial (P< 0·03). B, breakfast period, in which participants ate a prescribed breakfast during the breakfast trial and rested during the morning fasting trial. L, ad libitum pasta lunch. –▲–, Breakfast; –○–, fasting.
Fig. 3
Fig. 3
Hormonal responses during trials. (a) Plasma acylated and total ghrelin (n 32), (b) plasma peptide tyrosine–tyrosine (PYY, n 32), (c) plasma glucagon-like peptide-1 (GLP-1, n 32), (d) serum leptin (n 32), where missing data are due to insufficient blood for analysis. Values are means with their normalised CI represented by vertical bars. * Mean value was significantly different from the corresponding time point in other trial (P< 0·05). B, breakfast period, in which participants ate a prescribed breakfast during the breakfast trial and rested during the morning fasting trial. L, ad libitum pasta lunch. –▲–, Breakfast; –○–, fasting.
Fig. 4
Fig. 4
Appetite score during trials. B, breakfast period, in which participants ate a prescribed breakfast during the breakfast trial and rested during the morning fasting trial. L, ad libitum pasta lunch. n 34, as one individual was not provided with hedonic scales on one of their trials. Values are means with their normalised CI represented by vertical bars. * Mean value was significantly different from the corresponding time point in other trial (P< 0·01). –▲–, Breakfast; –○–, fasting.

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