Association between serum 25-hydroxyvitamin D and inflammatory cytokines in healthy adults

Xiaomin Sun, Zhen-Bo Cao, Yuping Zhang, Yoshiko Ishimi, Izumi Tabata, Mitsuru Higuchi, Xiaomin Sun, Zhen-Bo Cao, Yuping Zhang, Yoshiko Ishimi, Izumi Tabata, Mitsuru Higuchi

Abstract

Here, we aimed to examine the associations between levels of serum 25-hydroxyvitamin D [25(OH)D] and inflammatory cytokines in healthy Japanese adults. A total of 95 healthy adults (61 women; age range 21-69 years) participated in our study. Fasting blood samples were analyzed for 25(OH)D, 1,25-dihydroxyvitamin D [1,25(OH)2D], interferon-γ (IFN-γ), interleukin-6 (IL-6), and interleukin-17 (IL-17) levels using enzyme-linked immunosorbent assays kits. Total percent body fat was determined by dual energy X-ray absorptiometry (DXA). Moderate to vigorous physical activity (MVPA) was assessed objectively using an activity monitor for 7 days. The mean 25(OH)D concentration was 34.7 nmol/L, and 83 subjects had 25(OH)D concentrations less than 50 nmol/L. Multiple linear regression analysis revealed that serum 25(OH)D level was positively related to plasma IL-17 level (β=0.26, p=0.025), after adjustment for gender, age, vitamin D intake, alcohol consumption, smoking status, and percent body fat. This relationship remained statistically significant (β=0.28, p=0.019) even after additional adjustment for MVPA. However, no significant association was found between serum 25(OH)D level and plasma IFN-γ or IL-6 levels. In conclusion, this study identified a high prevalence of vitamin D deficiency in healthy Japanese adults. Serum 25(OH)D level was positively related to IL-17 level, independent of physical activity.

Figures

Figure 1
Figure 1
The relationship between serum 25(OH)D and IL-6 (A); IFN-γ (B); and IL-17 (C) in healthy Japanese adults. Data were logarithmic-transformed or # reciprocal-of-square-root transformed before performing the analysis. Open and closed circles represent data from women and men, respectively.

References

    1. Wacker M., Holick M.F. Vitamin D—Effects on skeletal and extraskeletal health and the need for supplementation. Nutrients. 2013;5:111–148. doi: 10.3390/nu5010111.
    1. Zittermann A., Gummert J.F. Nonclassical vitamin D actions. Nutrients. 2010;2:408–425. doi: 10.3390/nu2040408.
    1. Colin E.M., Asmawidjaja P.S., van Hamburg J.P., Mus A.M., van Driel M., Hazes J.M., van Leeuwen J.P., Lubberts E. 1,25-Dihydroxyvitamin D3 modulates Th17 polarization and interleukin-22 expression by memory T cells from patients with early rheumatoid arthritis. Arthritis Rheum. 2010;62:132–142. doi: 10.1002/art.25043.
    1. Correale J., Ysrraelit M., Gaitan M. Immunomodulatory effects of vitamin D in multiple sclerosis. Brain. 2009;132:1146–1160. doi: 10.1093/brain/awp033.
    1. Khoo A.L., Chai L.Y.A., Koenen H.J.P.M., Sweep F.C.G.J., Joosten I., Netea M.G., van der Ven A.J.A.M. Regulation of cytokine responses by seasonality of vitamin D status in healthy individuals. Clin. Exp. Immunol. 2011;164:72–79. doi: 10.1111/j.1365-2249.2010.04315.x.
    1. Zhu Y., Mahon B.D., Froicu M., Cantorna M.T. Calcium and 1α,25-dihydroxyvitamin D3 target the TNF-α pathway to suppress experimental inflammatory bowel disease. Eur. J. Immunol. 2004;35:217–224.
    1. Lemire J.M. Immunomodulatory role of 1,25-dihydroxyvitamin D3. J. Cell. Biochem. 1992;49:26–31. doi: 10.1002/jcb.240490106.
    1. Muller K., Diamant M., Bendtzen K. Inhibition of production and function of interleukin-6 by 1,25-dihydroxyvitamin D3. Immunol. Lett. 1991;28:115–120. doi: 10.1016/0165-2478(91)90108-M.
    1. Barker T., Martins T.B., Hill H.R., Kjeldsberg C.R., Dixon B.M., Schneider E.D., Henriksen V.T., Weaver L.K. Circulating pro-inflammatory cytokines are elevated and peak power output correlates with 25-hydroxyvitamin D in vitamin D insufficient adults. Eur. J. Appl. Physiol. 2013;113:1523–1534. doi: 10.1007/s00421-012-2582-7.
    1. Peterson C.A., Heffernan M.E. Serum tumor necrosis factor-α concentrations are negatively correlated with serum 25(OH)D concentrations in healthy women. J. Inflamm. 2008;5:10. doi: 10.1186/1476-9255-5-10.
    1. Miossec P., Korn T., Kuchroo V.K. Interleukin-17 and type 17 helper T cells. N. Engl. J. Med. 2009;361:888–898. doi: 10.1056/NEJMra0707449.
    1. Panagiotakos D.B., Pitsavos C., Chrysohoou C., Kavouras S., Stefanadis C. The associations between leisure-time physical activity and inflammatory and coagulation markers related to cardiovascular disease: The attica study. Prev. Med. 2005;40:432–437. doi: 10.1016/j.ypmed.2004.07.010.
    1. Golzari Z., Shabkhiz F., Soudi S., Kordi M.R., Hashemi S.M. Combined exercise training reduces IFN-γ and IL-17 levels in the plasma and the supernatant of peripheral blood mononuclear cells in women with multiple sclerosis. Int. Immunopharmacol. 2010;10:1415–1419. doi: 10.1016/j.intimp.2010.08.008.
    1. Scragg R., Camargo C.A., Jr. Frequency of leisure-time physical activity and serum 25-hydroxyvitamin D levels in the us population: Results from the third national health and nutrition examination survey. Am. J. Epidemiol. 2008;168:577–586; discussion 587–591. doi: 10.1093/aje/kwn163.
    1. Kumahara H., Schutz Y., Ayabe M., Yoshioka M., Yoshitake Y., Shindo M., Ishii K., Tanaka H. The use of uniaxial accelerometry for the assessment of physical-activity-related energy expenditure: A validation study against whole-body indirect calorimetry. Br. J. Nutr. 2004;91:235–243. doi: 10.1079/BJN20031033.
    1. Cao Z.B., Miyatake N., Higuchi M., Miyachi M., Ishikawa-Takata K., Tabata I. Predicting VO2max with an objectively measured physical activity in Japanese women. Med. Sci. Sports Exerc. 2010;42:179–186.
    1. Murakami K., Mizoue T., Sasaki S., Ohta M., Sato M., Matsushita Y., Mishima N. Dietary intake of folate, other B vitamins, and ω-3 polyunsaturated fatty acids in relation to depressive symptoms in Japanese adults. Nutrition. 2008;24:140–147. doi: 10.1016/j.nut.2007.10.013.
    1. Kobayashi S., Murakami K., Sasaki S., Okubo H., Hirota N., Notsu A., Fukui M., Date C. Comparison of relative validity of food group intakes estimated by comprehensive and brief-type self-administered diet history questionnaires against 16 days dietary records in Japanese adults. Public Health Nutr. 2011;14:1200–1211. doi: 10.1017/S1368980011000504.
    1. Nakanishi N., Suzuki K., Tatara K. Alcohol consumption and risk of development of impaired fasting glucose or type 2 diabetes in middle-aged Japanese men. Diabetes Care. 2003;26:48–54. doi: 10.2337/diacare.26.1.48.
    1. Nakamura K. Vitamin D insufficiency in Japanese populations: From the viewpoint of the prevention of osteoporosis. J. Bone Miner. Metab. 2006;24:1–6. doi: 10.1007/s00774-005-0637-0.
    1. Lips P., Hosking D., Lippuner K., Norquist J.M., Wehren L., Maalouf G., Ragi-Eis S., Chandler J. The prevalence of vitamin D inadequacy amongst women with osteoporosis: An international epidemiological investigation. J. Int. Med. 2006;260:245–254. doi: 10.1111/j.1365-2796.2006.01685.x.
    1. Nakamura K., Tsugawa N., Saito T., Ishikawa M., Tsuchiya Y., Maruyama K., Oshiki R., Kobayashi R., Nashimoto M., Yoshihara A., et al. Vitamin D status, bone mass, and bone metabolism in home-dwelling postmenopausal Japanese women: Yokogoshi study. Bone. 2008;42:271–277. doi: 10.1016/j.bone.2007.09.056.
    1. Nakamura K., Nashimoto M., Hori Y., Muto K., Yamamoto M. Serum 25-hydroxyvitamin D levels in active women of middle and advanced age in a rural community in Japan. Nutrition. 1999;15:870–873. doi: 10.1016/S0899-9007(99)00208-7.
    1. Nakamura K., Nashimoto M., Matsuyama S., Yamamoto M. Low serum concentrations of 25-hydroxyvitamin D in young adult Japanese women: A cross sectional study. Nutrition. 2001;17:921–925. doi: 10.1016/S0899-9007(01)00662-1.
    1. Holick M.F. Vitamin D and health: Evolution, biologic functions, and recommended dietary intakes for vitamin D. Clin. Rev. Bone Miner. Metab. 2009;7:2–19. doi: 10.1007/s12018-009-9026-x.
    1. Holick M.F. Vitamin D deficiency. N. Engl. J. Med. 2007;357:266–281. doi: 10.1056/NEJMra070553.
    1. Holick M.F., Chen T.C. Vitamin D deficiency: A worldwide problem with health consequences. Am. J. Clin. Nutr. 2008;87:1080S–1086S.
    1. Japanese Ministry of Health, Labour and Welfare . Dietary Reference Intakes for Japanese, 2010. Daiichi Shuppan Publishing Co. Ltd.; Tokyo, Japan: 2009.
    1. Maalmi H., Berraies A., Tangour E., Ammar J., Abid H., Hamzaoui K., Hamzaoui A. The impact of vitamin D deficiency on immune T cells in asthmatic children: A case-control study. J. Asthma Allergy. 2012;5:11–19.
    1. Onishi R.M., Gaffen S.L. Interleukin-17 and its target genes: Mechanisms of interleukin-17 function in disease. Immunology. 2010;129:311–321. doi: 10.1111/j.1365-2567.2009.03240.x.
    1. Wilson N.J., Boniface K., Chan J.R., McKenzie B.S., Blumenschein W.M., Mattson J.D., Basham B., Smith K., Chen T., Morel F., et al. Development, cytokine profile and function of human interleukin 17-producing helper T cells. Nat. Immunol. 2007;8:950–957. doi: 10.1038/ni1497.
    1. Liu P.T., Stenger S., Li H.Y., Wenzel L., Tan B.H., Krutzik S.R., Ochoa M.T., Schauber J., Wu K., Meinken C., et al. Toll-like receptor triggering of a vitamin D-mediated human antimicrobial response. Science. 2006;311:1770–1773. doi: 10.1126/science.1123933.
    1. Bhan I., Camargo C.A., Jr., Wenger J., Ricciardi C., Ye J., Borregaard N., Thadhani R. Circulating levels of 25-hydroxyvitamin D and human cathelicidin in healthy adults. J. Allergy Clin. Immunol. 2011;127:1302–1304.
    1. Dixon B.M., Barker T., Mckinnon T., Cuomo J., Frei B., Borregaard N., Gombart A.F. Positive correlation between circulating cathelicidin antimicrobial peptide (hCAP/LL-37) and 25-hydroxyvitamin D levels in healthy adults. BMC Res. Notes. 2012;5:575. doi: 10.1186/1756-0500-5-575.
    1. Peric M., Koglin S., Kim S.M., Morizane S., Besch R., Prinz J.C., Ruzicka T., Gallo R.L., Schauber J. IL-17a enhances vitamin D3-induced expression of cathelicidin antimicrobial peptide in human keratinocytes. J. Immunol. 2008;181:8504–8512.
    1. Robinson-Cohen C., Hoofnagle A.N., Ix J.H., Sachs M.C., Tracy R.P., Siscovick D.S., Kestenbaum B.R., de Boer I.H. Racial differences in the association of serum 25-hydroxyvitamin D concentration with coronary heart disease events. JAMA. 2013;310:179–188. doi: 10.1001/jama.2013.7228.

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