Preclinical safety assessment of MV-s-NAP, a novel oncolytic measles virus strain armed with an H . pylori immunostimulatory bacterial transgene
Kimberly B Viker, Michael B Steele, Ianko D Iankov, Susanna C Concilio, Arun Ammayappan, Brad Bolon, Nathan J Jenks, Matthew P Goetz, Eleni Panagioti, Mark J Federspiel, Minetta C Liu, Kah Whye Peng, Evanthia Galanis, Kimberly B Viker, Michael B Steele, Ianko D Iankov, Susanna C Concilio, Arun Ammayappan, Brad Bolon, Nathan J Jenks, Matthew P Goetz, Eleni Panagioti, Mark J Federspiel, Minetta C Liu, Kah Whye Peng, Evanthia Galanis
Abstract
Despite recent therapeutic advances, metastatic breast cancer (MBC) remains incurable. Engineered measles virus (MV) constructs based on the attenuated MV Edmonston vaccine platform have demonstrated significant oncolytic activity against solid tumors. The Helicobacter pylori neutrophil-activating protein (NAP) is responsible for the robust inflammatory reaction in gastroduodenal mucosa during bacterial infection. NAP attracts and activates immune cells at the site of infection, inducing expression of pro-inflammatory mediators. We engineered an MV strain to express the secretory form of NAP (MV-s-NAP) and showed that it exhibits anti-tumor and immunostimulatory activity in human breast cancer xenograft models. In this study, we utilized a measles-infection-permissive mouse model (transgenic IFNAR KO-CD46Ge) to evaluate the biodistribution and safety of MV-s-NAP. The primary objective was to identify potential toxic side effects and confirm the safety of the proposed clinical doses of MV-s-NAP prior to a phase I clinical trial of intratumoral administration of MV-s-NAP in patients with MBC. Both subcutaneous delivery (corresponding to the clinical trial intratumoral administration route) and intravenous (worst case scenario) delivery of MV-s-NAP were well tolerated: no significant clinical, laboratory or histologic toxicity was observed. This outcome supports the safety of MV-s-NAP for oncolytic virotherapy of MBC. The first-in-human clinical trial of MV-s-NAP in patients with MBC (ClinicalTrials.gov: NCT04521764) was subsequently activated.
Keywords: IFNAR KO-CD46Ge mouse model; MV-s-NAP; NAP; measles; metastatic breast cancer; neutrophil activating protein; oncolytic virus.
Conflict of interest statement
E.G. receives personal compensation as an advisory board member from Kiyatec, Inc., and compensation paid to employer from Karyopharm Therapeutics, Inc. She also receives grant/research/clinical trial funding paid to employer from Servier Pharmaceuticals LLC (formerly Agios Pharmaceuticals, Inc.), Celgene, MedImmune, Inc., and Tracon Pharmaceuticals. K.W.P. and the Mayo Clinic have a financial interest in the technology used in this research. The remaining authors cite no conflicts of interest.
© 2022 Mayo Clinic.
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Treatment with MV-s-NAP does not…
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Treatment with MV-s-NAP does not increase circulating levels of pro-inflammatory cytokines in plasma…
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Quantification of MV-s-NAP genomes recovered…
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Quantification of MV-s-NAP genomes recovered from tissues as detected by quantitative real-time reverse…
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Minor histopathological findings in MV-s-NAP…
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Minor histopathological findings in MV-s-NAP treated mice (A) H&E stain of lung tissue.…
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Antibody responses to measles virus…
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Antibody responses to measles virus and NAP protein Antibody production against MV or…
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Schema of the phase I…
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Schema of the phase I clinical trial of intratumoral MV-s-NAP administration to treat…
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- Chia S., Bedard P.L., Hilton J., Amir E., Gelmon K., Goodwin R., Villa D., Cabanero M., Tu D., Tsao M., Seymour L. A phase ib trial of durvalumab in combination with trastuzumab in HER2-positive metastatic breast cancer (CCTG IND.229) Oncologist. 2019;24:1439–1445. doi: 10.1634/theoncologist.2019-0321. - DOI - PMC - PubMed
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- Mittendorf E.A., Lu B., Melisko M., Price Hiller J., Bondarenko I., Brunt A.M., Sergii G., Petrakova K., Peoples G.E. Efficacy and safety analysis of nelipepimut-S vaccine to prevent breast cancer recurrence: a randomized, multicenter, phase III clinical trial. Clin. Cancer Res. 2019;25:4248–4254. doi: 10.1158/1078-0432.Ccr-18-2867. - DOI - PubMed
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Figure 4
Quantification of MV-s-NAP genomes recovered…
Figure 4
Quantification of MV-s-NAP genomes recovered from tissues as detected by quantitative real-time reverse…
Figure 5
Minor histopathological findings in MV-s-NAP…
Figure 5
Minor histopathological findings in MV-s-NAP treated mice (A) H&E stain of lung tissue.…
Figure 6
Antibody responses to measles virus…
Figure 6
Antibody responses to measles virus and NAP protein Antibody production against MV or…
Figure 7
Schema of the phase I…
Figure 7
Schema of the phase I clinical trial of intratumoral MV-s-NAP administration to treat…
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