Plasma ctDNA Detection in Diagnosis of Epithelial Ovarian Cancer. (ctDNA_EOC)
研究概览
详细说明
Epithelial ovarian cancer constitutes one of the most common gynecological malignancies, with an incidence rate of 3-12/100 000 woman per year. Because the ovaries lie in the deep pelvic cavity,most ovarian cancer patients are asymptomatic, rendering the majority often diagnosed at an advanced stage. Liquid biopsy, which is meant to detect cancers by sequencing the DNA in a few drops of a person's blood. It may detect cancers early, even before symptoms arise, when there is just a few cells in the blood circulation.
ctDNA in cancer patients often bears similar genetic and epigenetic features to the related tumor DNA. There is evidence that some of the ctDNA originates from tumor tissue. This, and the fact that ctDNA can easily be isolated from the circulation and other body fluids of patients, makes it a promising candidate as a non-invasive biomarker of cancer.
This study aims to detect plasma ctDNA in Diagnosis of Epithelial Ovarian Cancer.
研究类型
注册 (实际的)
联系人和位置
学习地点
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Shanghai
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Shanghai、Shanghai、中国、200126
- Renji Hospital, School of Medicine, Shanghai Jiao Tong University
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参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
取样方法
研究人群
描述
Inclusion Criteria:
- people aged 18years to 80years;
- Diagnosis of ovarian cancer is confirmed by two Pathologists independently;
- Pregnancy tests are negative.
Exclusion Criteria:
- With other type of malignancies;
- someone who refuse to participate the study.
学习计划
研究是如何设计的？
设计细节
队列和干预
团体/队列 |
干预/治疗 |
|---|---|
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Epithelial ovarian cancer
patients receive the ctDNA methylation markers screening
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ctDNA extraction from plasma samples;bisulfite-treated DNA;DNA methylation levels by deep sequencing-Sequencing; data analysis
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研究衡量的是什么？
主要结果指标
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
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Pathological diagnosis of ovarian lumps
大体时间：One week after surgery
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Pathological diagnosis of ovarian lumps，including immunohistochemical information and HE staining information.
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One week after surgery
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次要结果测量
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
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sensitivity (true positive rate)
大体时间：2 year
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The proportion of persons with disease who have a positive test (positive test results among persons with disease)
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2 year
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Specificity (true negative rate)
大体时间：2 year
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The proportion of persons without disease who have a negative test (negative test results among persons without disease)
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2 year
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合作者和调查者
调查人员
- 首席研究员：Yu Wang, M.D.、Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University
出版物和有用的链接
一般刊物
- Wen L, Li J, Guo H, Liu X, Zheng S, Zhang D, Zhu W, Qu J, Guo L, Du D, Jin X, Zhang Y, Gao Y, Shen J, Ge H, Tang F, Huang Y, Peng J. Genome-scale detection of hypermethylated CpG islands in circulating cell-free DNA of hepatocellular carcinoma patients. Cell Res. 2015 Nov;25(11):1250-64. doi: 10.1038/cr.2015.126. Epub 2015 Oct 30. Erratum In: Cell Res. 2015 Dec;25(12):1376.
- Nick AM, Coleman RL, Ramirez PT, Sood AK. A framework for a personalized surgical approach to ovarian cancer. Nat Rev Clin Oncol. 2015 Apr;12(4):239-45. doi: 10.1038/nrclinonc.2015.26. Epub 2015 Feb 24.
研究记录日期
研究主要日期
学习开始 (实际的)
初级完成 (实际的)
研究完成 (实际的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (实际的)
研究记录更新
最后更新发布 (实际的)
上次提交的符合 QC 标准的更新
最后验证
更多信息
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