Humidified Culture and Live Birth After ICSI

June 9, 2020 updated by: Muhammad Fawzy, Ibn Sina Hospital

Humidified Incubator Effect on Live Birth Rate After ICSI

Humidity for human embryo culture has been raised as essential with advantageous effect on clinical pregnancy and ongoing pregnancy after ICSI compared with a dry incubator. Whether the deleterious effect of dry culture would have an extended effect reaches the live birth rate needs to be examined. This RCT is to examine the effect of dry and humidified culture on live birth rates after ICSI.

Study Overview

Status

Status

Indicates the current recruitment status or the expanded access status.
Completed

Conditions

Conditions

The disease, disorder, syndrome, illness, or injury that is being studied. On ClinicalTrials.gov, conditions may also include other health-related issues, such as lifespan, quality of life, and health risks.

Intervention / Treatment

Intervention / Treatment

A process or action that is the focus of a clinical study. Interventions include drugs, medical devices, procedures, vaccines, and other products that are either investigational or already available. Interventions can also include noninvasive approaches, such as education or modifying diet and exercise.

Study Type

Study Type

Describes the nature of a clinical study. Study types include interventional studies (also called clinical trials), observational studies (including patient registries), and expanded access.
Interventional

Enrollment (Actual)

Enrollment

The number of participants in a clinical study. The "anticipated" enrollment is the target number of participants that the researchers need for the study.
1837

Phase

Phase

The stage of a clinical trial studying a drug or biological product, based on definitions developed by the U.S. Food and Drug Administration (FDA). The phase is based on the study's objective, the number of participants, and other characteristics. There are five phases: Early Phase 1 (formerly listed as Phase 0), Phase 1, Phase 2, Phase 3, and Phase 4. Not Applicable is used to describe trials without FDA-defined phases, including trials of devices or behavioral interventions.
  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

  • Egypt
      • Assiut, Egypt
        • Banon Assiut
      • Qena, Egypt, 123456
        • Qena Fertility Center
      • Sohag, Egypt
        • IbnSina IVF Center

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Eligibility Criteria

The key requirements that people who want to participate in a clinical study must meet or the characteristics they must have. Eligibility criteria consist of both inclusion criteria (which are required for a person to participate in the study) and exclusion criteria (which prevent a person from participating). Types of eligibility criteria include whether a study accepts healthy volunteers, has age or age group requirements, or is limited by sex.

Ages Eligible for Study

18 years to 40 years (Adult)

Accepts Healthy Volunteers

No

Genders Eligible for Study

Female

Description

Inclusion Criteria:

  • Women undergoing a first or second ICSI attempts
  • Primary or secondary infertility with a BMI of < 31 kg/m2
  • Duration of infertility of ≥ 18 months and normal baseline ultrasonography
  • 18 to 40 years of age
  • Tubal factor, male factor, polycystic ovary, unexplained infertility or combined factors
  • Tubal factors

Exclusion Criteria:

  • Surgically-retrieved, frozen-thawed and pinpoint sperm or globozoospermia
  • Thin endometrium
  • Uterine anomaly and adhesion
  • Adenomyosis
  • Submucous myoma
  • Endometriosis
  • Severe medical condition

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Other
  • Allocation: Randomized
  • Interventional Model: Parallel Assignment
  • Masking: Quadruple

Arms and Interventions

Participant Group / Arm

Participant Group / Arm

A group or subgroup of participants in a clinical trial that receives a specific intervention/treatment, or no intervention, according to the trial's protocol.
Intervention / Treatment

Intervention / Treatment

A process or action that is the focus of a clinical study. Interventions include drugs, medical devices, procedures, vaccines, and other products that are either investigational or already available. Interventions can also include noninvasive approaches, such as education or modifying diet and exercise.
Experimental: Culture of human embryo in non-humidified incubator
Embryo culture after in vitro fertilization in incubator with no humidity
Other: Human embryo culture
humidifed or dry incubator
No Intervention: Culture of human embryo in humidified incubator
Embryo culture after in vitro fertilization in incubator with humidity

What is the study measuring?

Primary Outcome Measures

Primary Outcome Measures

In a clinical study's protocol, the planned outcome measure that is the most important for evaluating the effect of an intervention/treatment. Most clinical studies have one primary outcome measure, but some have more than one.
Outcome Measure
Measure Description
Time Frame
Live birth rate
Time Frame: 30 weeks
Viable neonate after 30 weeks of gestation per initiated cycle
30 weeks

Secondary Outcome Measures

Secondary Outcome Measures

In a clinical study's protocol, a planned outcome measure that is not as important as the primary outcome measure for evaluating the effect of an intervention but is still of interest. Most clinical studies have more than one secondary outcome measure.
Outcome Measure
Measure Description
Time Frame
Cumulative live birth rate
Time Frame: One year
Viable neonate after 30 weeks of gestation after one fresh and/or one frozen-warmed transfer per initiated cycle
One year
Ongoing pregnancy rate
Time Frame: 12 weeks of gestation
Continued pregnancy after 12 weeks of gestation per initiated cycle
12 weeks of gestation
Cumulative ongoing pregnancy rate
Time Frame: 12 weeks of gestation
Continued pregnancy after 12 weeks of gestation per initiated cycle within one year of randomization
12 weeks of gestation
Clinical pregnancy rate
Time Frame: 7 weeks of gestation
Ultrasound detect heartbeat after 7 weeks of gestation per initiated cycle
7 weeks of gestation
Cumulative clinical pregnancy rate
Time Frame: 7 weeks of gestation
Ultrasound detect heartbeat after 7 weeks of gestation per initiated cycle within one year of randomization
7 weeks of gestation
Implantation rate
Time Frame: 7 weeks of gestation
Number of sacs with a heartbeat per number of embryo transferred
7 weeks of gestation
Cumulative implantation rate
Time Frame: 7 weeks of gestation
Number of sacs with a heartbeat per number of embryo transferred within one year of randomization
7 weeks of gestation
Ongoing implantation rate
Time Frame: 12 weeks of gestation
Number of sacs with a heartbeat per number of embryo transferred
12 weeks of gestation
Cumulative ongoing implantation rate
Time Frame: 12 weeks of gestation
Number of sacs with a heartbeat per number of embryo transferred within one year of randomization
12 weeks of gestation
Preterm birth rate
Time Frame: 37 weeks
number of babies born before 37 weeks of gestation per total birth
37 weeks
Very preterm birth
Time Frame: 32 weeks
number of babies born before 32 weeks of gestation per total birth
32 weeks
Low birth weight rate
Time Frame: 40 weeks of gestation
number of babies with < 2500 gm per total birth
40 weeks of gestation
Fertilization rate
Time Frame: 6 days of culture
fertilized oocytes with two pronuclei per Metaphase II injected
6 days of culture
Cleavage rate
Time Frame: 6 days of culture
Cleaved embryo per fertilized oocyte
6 days of culture
Rate of high-quality day-3 embryos
Time Frame: 6 days of culture
Embryos with seven or eight blastomeres of appropriate-size, and < 10% fragmentation by volume per fertilized oocyte
6 days of culture
Blastocyst formation rate
Time Frame: 6 days of culture
Formed blastocyst on day 5/6 from fertilized oocytes
6 days of culture
Rate of high-quality blastocyst
Time Frame: 6 days of culture
Blastocysts ≥ 3.1.1 grade per fertilized oocyte
6 days of culture
Rate of utilizable blastocyst
Time Frame: 6 days of culture
Transferred and cryopreserved blastocysts from fertilized oocytes
6 days of culture

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Sponsor

The organization or person who initiates the study and who has authority and control over the study.
Ibn Sina Hospital

Collaborators

Collaborators

An organization other than the sponsor that provides support for a clinical study. This support may include activities related to funding, design, implementation, data analysis, or reporting.
Banoon IVF Center
Quena IVF Center
Amshag IVF Center

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

Study Start

The actual date on which the first participant was enrolled in a clinical study. The "anticipated" study start date is the date that the researchers think will be the study start date.
February 1, 2019

Primary Completion (Actual)

Primary Completion

The date on which the last participant in a clinical study was examined or received an intervention to collect final data for the primary outcome measure. Whether the clinical study ended according to the protocol or was terminated does not affect this date. For clinical studies with more than one primary outcome measure with different completion dates, this term refers to the date on which data collection is completed for all the primary outcome measures. The "anticipated" primary completion date is the date that the researchers think will be the primary completion date for the study.
March 27, 2020

Study Completion (Actual)

Study Completion

The date on which the last participant in a clinical study was examined or received an intervention/treatment to collect final data for the primary outcome measures, secondary outcome measures, and adverse events (that is, the last participant's last visit). The "anticipated" study completion date is the date that the researchers think will be the study completion date.
March 27, 2020

Study Registration Dates

First Submitted

First Submitted

The date on which the study sponsor or investigator first submitted a study record to ClinicalTrials.gov. There is typically a delay of a few days between the first submitted date and the record's availability on ClinicalTrials.gov (the first posted date).
January 27, 2019

First Submitted That Met QC Criteria

First Submitted That Met QC Criteria

The date on which the study sponsor or investigator first submits a study record that is consistent with National Library of Medicine (NLM) quality control (QC) review criteria. The sponsor or investigator may need to revise and submit a study record one or more times before NLM's QC review criteria are met. It is the responsibility of the sponsor or investigator to ensure that the study record is consistent with the NLM QC review criteria.
January 29, 2019

First Posted (Actual)

First Posted

The date on which the study record was first available on ClinicalTrials.gov after National Library of Medicine (NLM) quality control (QC) review has concluded. There is typically a delay of a few days between the date the study sponsor or investigator submitted the study record and the first posted date.
January 30, 2019

Study Record Updates

Last Update Posted (Actual)

Last Update Posted

The most recent date on which changes to a study record were made available on ClinicalTrials.gov. There may be a delay between when the changes were submitted to ClinicalTrials.gov by the study's sponsor or investigator (the last update submitted date) and the last update posted date.
June 11, 2020

Last Update Submitted That Met QC Criteria

Last Update Submitted That Met QC Criteria

The most recent date on which the study sponsor or investigator submitted changes to a study record that are consistent with National Library of Medicine (NLM) quality control (QC) review criteria. It is the responsibility of the sponsor or investigator to ensure that the study record is consistent with the NLM QC review criteria.
June 9, 2020

Last Verified

Last Verified

The most recent date on which the study sponsor or investigator confirmed the information about a clinical study on ClinicalTrials.gov as accurate and current. If a study with a recruitment status of recruiting; not yet recruiting; or active, not recruiting has not been confirmed within the past 2 years, the study's recruitment status is shown as unknown.
June 1, 2020

More Information

Terms related to this study

Additional Relevant MeSH Terms

Other Study ID Numbers

Other Study ID Numbers

Identifiers or ID numbers other than the NCT number that are assigned to a clinical study by the study's sponsor, funders, or others. These numbers may include unique identifiers from other trial registries and National Institutes of Health grant numbers.
  • IbnSina-Humidity

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

NO

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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