Study of Diagnosis and Pathophysiology of Pulmonary Embolism (APE 1 Trial)

The Acute Pulmonary Embolism 1 (APE 1) Trial: Prospective Investigation of Scintigraphic Diagnosis and Pathophysiology of Right Heart Strain

The purpose of this study is to

• investigate which method and criterion for diagnosing pulmonary embolism is the best and
• determine the relationship between blood vessel constriction and clot size in patients developing heart failure

Study Overview

• Status: Terminated
• Conditions: Pulmonary Embolism; Right Heart Strain
• Intervention / Treatment: Procedure: Scintigraphic interpretation

Detailed Description

INTRODUCTION This project assesses both scintigraphic diagnosis and the pathophysiology of right heart strain in acute pulmonary embolism. The primary purposes are to validate various scintigraphic methods with pulmonary angiography as the standard of reference (purpose A). Furthermore, this project examines the relative contributions of 1) vascular obstruction, 2) vasoactive mediators, and 3) co-morbidity to the generation of right heart strain (purpose B).

BACKGROUND FOR PURPOSE A Acute pulmonary embolism is a life threatening condition. Early intervention can be lifesaving but treatment is associated with severe side effects. Thus, establishing accurate diagnosis is necessary. Clinical presentation and physical examination have proven insufficient for this purpose. This is also true for basic diagnostic investigations, e.g. electrocardiography, arterial blood gas analysis and chest x-ray, which are considered standard tests in patients with symptoms consistent with cardiopulmonary disease.

For several decades lung scintigraphy has been considered a cornerstone in the diagnosis of acute pulmonary embolism. Planar two-dimensional images of the pulmonary perfusion are obtained using a gamma camera after intravenous injection of radiolabelled microparticles, depicting perfusion defects caused by e.g. a pulmonary embolus. Similarly, ventilatory function can be assessed two-dimensionally by planar scintigraphic imaging following inhalation of a radioactive gas or aerosol. Lung scintigraphy has certain limitations, primarily related to image interpretation. The PIOPED criteria, based on the combined ventilation-perfusion scintigraphy, have gained global acceptance, but are often inconclusive and give rise to misunderstandings. A different set of interpretation criteria, provided by the PISA-PED study group, are based solely on perfusion imaging. Opposite the PIOPED criteria, PISA-PED criteria are always conclusive and exhibits both high sensitivity and specificity. The latter have, however, not gained the same widespread acceptance as the PIOPED criteria. It remains uncertain which set of criteria is the better to confirm or exclude the diagnosis of acute pulmonary. A prospective trial evaluating both PIOPED criteria (original and revised) and PISA-PED criteria against a valid standard of reference therefore seems appropriate.

The more resource demanding single photon emission computed tomography (SPECT) has recently been subject to renewed interest in the diagnosis of acute pulmonary embolism, but it remains unclear whether this technique offers additional information compared to conventional planar imaging. SPECT offers three-dimensional images, but otherwise the technique is similar to the methods described for planar images. Combining the PISA-PED criteria and SPECT imaging has not yet been tested in a prospective trial, but it seems highly relevant.

Since the diagnosis is often suspected several times in the same patient, the ability to distinguish acute and chronic changes in the scintigrams are essential. It is, however, unclear to what extend and how fast the scintigraphic changes resolve. This has great implications in scintigraphic control of patients recovering from acute pulmonary embolism.

A trial regarding the statements above requires a reliable method to test whether the patient is suffering from acute pulmonary embolism or not: a standard of reference. Pulmonary angiography is traditionally conceived as the standard of reference in acute pulmonary embolism, but only a few newer studies employ this technique.

BACKGROUND FOR PURPOSE B Right heart strain is a common finding in acute pulmonary embolism. It is a result of the sudden increase in pressure in the pulmonary circulation. Ultrasonographic examination of the heart by echocardiography is suitable to demonstrate this condition and this finding in a patient with acute pulmonary embolism is associated with a considerably worse prognosis. The degree of right heart strain is not exclusively determined by extension or size of the embolus. There is substantial evidence of the influence of both co-morbidity and the release of vasoactive mediators on the extend of right heart strain. The exact interplay between these three factors is not completely understood in humans. Albeit, this can have significant implications on therapy, since pharmacological manipulation of vascular tone in the pulmonary circulation is possible. This treatment is, however, not without risk, and no well-designed study has yet addressed these issues in patients with acute pulmonary embolism. Several case reports on successful treatment with vasodilation, e.g. with inhaled nitric oxide, have been published. A more profound understanding of the etiology of acute right heart failure in acute pulmonary embolism is therefore desirable. What is the individual contribution of 1) clot burden, 2) release of vasoactive mediators, and 3) co-morbidity?

Recently, acute pulmonary embolism has been divided into three categories depending on clinical presentation and echocardiographic findings: 1) massive; in which the patient suffers from cardiac arrest or cardiogenic shock. Most authorities agree that these patients should be treated with fibrinolytic agents. 2) Non-massive; with no signs of shock or right heart strain. In these patients there is no indication for fibrinolytic agents. 3) Sub-massive; with no manifestations of shock but echocardiographic findings consistent with right heart strain. The indication for fibrinolytic treatment in this last group is unresolved. This study will address whether simple methods, e.g. peripheral blood sampling and perfusion scintigraphy can determine the primary mechanism in the generation of right heart strain in the individual patient.

A later (not yet planned) intervention study based on the abovementioned knowledge could then address the following question: Should the patient with sub-massive pulmonary embolism receive treatment with fibrinolytic agents (in the case of major clot burden and minor degree of pulmonary vasoconstriction) or vasodilators (minor clot burden, major degree of pulmonary vasoconstriction)?

• Study Type: Interventional
• Enrollment (Actual): 15
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Denmark
  • Odense C, Denmark, DK-5000
    • Odense University Hospital

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Referred from clinical departments at Odense University Hospital
• Referred to the Departments of Nuclear Medicine or Radiology for diagnostic evaluation of suspected pulmonary embolism
• Referred for lung scintigraphy, spiral computer tomography, or pulmonary angiography

Exclusion Criteria:

• Age below 18
• Contrast allergy
• Pregnancy
• S-Creatinine above 200 micromol/L
• Metformin treatment
• Fibrinolytic or surgical therapy between examinations
• No informed consent
• Withdrawn consent
• Failed logistics (more than 24 hours between examinations)
• No conclusive pulmonary angiography

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Diagnostic
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Other: None relevant; Not relevant	Procedure: Scintigraphic interpretation; Not relevant

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Purpose A: Specificity, sensitivity, predictive values, and observer variation for scintigraphic procedures	2006-2012
Purpose B: Blood concentrations of vasoactive substances, clot size on scintigram.	2006-2012

Collaborators and Investigators

• Sponsor: Odense University Hospital
• Collaborators: University of Southern Denmark
• Investigators: Principal Investigator: Poul Henning Madsen, MD, Department of Nuclear Medicine, Odense University Hospital; Principal Investigator: Soeren Hess, MD, Department of Nuclear Medicine, Odense University Hospital

Study record dates

Study Major Dates

• Study Start: April 1, 2006
• Primary Completion (Actual): December 1, 2012
• Study Completion (Actual): December 1, 2012

Study Registration Dates

• First Submitted: March 13, 2006
• First Submitted That Met QC Criteria: March 13, 2006
• First Posted (Estimate): March 14, 2006

Study Record Updates

• Last Update Posted (Estimate): April 18, 2016
• Last Update Submitted That Met QC Criteria: April 15, 2016
• Last Verified: April 1, 2016

More Information

Terms related to this study

• Keywords: Pulmonary embolism; Lung scintigraphy; Pulmonary angiography; Right heart strain
• Additional Relevant MeSH Terms: Cardiovascular Diseases; Vascular Diseases; Respiratory Tract Diseases; Lung Diseases; Wounds and Injuries; Embolism and Thrombosis; Embolism; Sprains and Strains; Pulmonary Embolism
• Other Study ID Numbers: 70

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO