Endostatin Serum Levels During Bicycle Stress Test

Endostatin Serum Levels During Bicycle Stress Test in Different Samples

Endostatin, a 20-kDa cleavage product of collagen XVIII, is a component of the extracellular matrix expressed in the basement membrane. As a potent inhibitor of angiogenesis, endostatin induces endothelial cell apoptosis and diminishes cell migration, adhesion and proliferation.

Endostatin may stop the progression of atherosclerosis. Atherosclerotic heart disease involves unwanted tissue growth. By cutting off the blood supply from a plaque the likelihood of plaque rupture may eventually be reduced. Recent data indicates that the loss of collagen XVIII/endostatin is related to the enhancement of neo-vascularization and vascular permeability in atherosclerosis. Plaque neo-vascularization strongly correlates with the regional content of inflammatory cells. Furthermore, increased vascular permeability enhances lipid accumulation in the vessel walls, hence increasing foam cells.

Therapeutic angiogenesis is a most promising strategy for the treatment of myocardial infarction. However, it remains unknown if and how endogenous angiogenesis inhibitors, such as endostatin, regulate angiogenesis in myocardial infarction. Rat models showed that after myocardial infarction endostatin neutralization displayed adverse left ventricular remodeling and severe heart failure compared with controls. Although angiogenesis was increased, tissue remodeling and interstitial fibrosis were further exaggerated in post-myocardial infarction hearts by endostatin neutralization.

However, several studies suggest that endostatin may locally modulate coronary collateral formation by inhibiting collateral vessel formation in patients with ischemic heart disease.

During treadmill exercise tests in healthy volunteers a significant increase in circulating endostatin levels can be observed. Exercise induces angiogenesis in cardiac and skeletal muscles by decreasing endostatin in the muscle tissues to increase blood flow to these metabolically active tissues. Thereby endostatin is released into the general circulation.

In summary, endostatin might be a new weapon to fight against atherosclerotic progression by inhibiting neo-vascularization of atherosclerotic plaques.

Study Overview

• Status: Completed
• Conditions: Smoking; Cardiac Diseases

• Study Type: Observational
• Enrollment (Actual): 240

Contacts and Locations

Study Locations

• Austria
  • Vienna, Austria, 1090
    • Medical University of Vienna

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 75 years (Adult, Older Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

• Sampling Method: Probability Sample

Study Population

200 patients, divided into sub-groups, always both genders will be tested for different conditions (smoking, age, CMP, CHD,...)

Description

Inclusion Criteria:

• Smoking/Non smoking
• Healthy/non healthy (if for CMP, CHD study)
• Age (depending on the group affiliation)

Exclusion Criteria:

• Suffering from grave diseases

Study Plan

How is the study designed?

Number of groups / cohorts

13

Cohorts and Interventions

Group / Cohort
Healthy young females; 20 healthy females, aged between 18 and 35 years
Healthy young males; 20 healthy males, aged between 18 and 35 years
Healthy elderly smokers; 20 healthy smokers, aged between 45 and 75 years
Healthy elderly non-smokers; 20 healthy non-smokers, aged between 45 and 75 years
Healthy young female smokers; 20 healthy female smokers, aged between 18 and 35 years
Healthy young male smokers; 20 healthy male smokers, aged between 18 and 35 years
Healthy postmenopausal women; 20 healthy postmenopausal women
Female CMP Patients; 20 female patients suffering from cardiomyopathy (ischemic or dilating)
Male CMP Patients; 20 male patients suffering from cardiomyopathy (ischemic or dilating)
Female CHD patients; 30 female patients suffering from cardiac heart disease, before aorto-coronary bypass surgery (and after)
Male CHD Patients; 30 male patients suffering from cardiac heart disease, before aorto-coronary bypass surgery (and after)
male athlets; 20 male athlets
female athlets; 20 female athlets

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Endostatin	baseline sample will be drawn at rest; a second sample will be drawn 5 minutes after each individual reaches its peak workload (average time 10 minutes)	baseline/maximum

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
catecholamine	baseline sample will be drawn at rest	baseline
hemodynamic parameters	heart rate and blood pressure behavior will be monitored throughout the entire bicycle stress test	baseline
catecholamine	a second sample will be drawn 5 minutes after each individual reaches its peak workload (average time 10 minutes)	day 1

Collaborators and Investigators

• Sponsor: Medical University of Vienna
• Investigators: Principal Investigator: Jeanette Strametz-Juranek, MD, MUV, Department of Internal Medicine II, Division of Cardiology

Publications and helpful links

General Publications

• Sponder M, Dangl D, Kampf S, Fritzer-Szekeres M, Strametz-Juranek J. Exercise increases serum endostatin levels in female and male patients with diabetes and controls. Cardiovasc Diabetol. 2014 Jan 6;13:6. doi: 10.1186/1475-2840-13-6.

Study record dates

Study Major Dates

• Study Start: January 1, 2008
• Primary Completion (Actual): December 1, 2012
• Study Completion (Actual): April 1, 2013

Study Registration Dates

• First Submitted: July 13, 2010
• First Submitted That Met QC Criteria: July 19, 2010
• First Posted (Estimate): July 20, 2010

Study Record Updates

• Last Update Posted (Estimate): May 20, 2014
• Last Update Submitted That Met QC Criteria: May 18, 2014
• Last Verified: May 1, 2014

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Cardiovascular Diseases; Heart Diseases
• Other Study ID Numbers: 220/2007