Post-exercise Dietary Protein Strategies

March 18, 2011 updated by: McMaster University

The Effect of the Pattern Post-exercise Aminoacidemia on Myofibrillar Protein Synthesis

Protein ingestion increases the rate at which the body builds new proteins in skeletal muscle (muscle protein synthesis. This study is designed to examine how the pattern of feeding affects muscle protein synthesis following resistance exercise. There is reason to believe that the large rapid increase in blood amino acid concentrations that accompanies the ingestion of a bolus of protein is important to increasing muscle protein synthesis. Thus, we hypothesize that the consumption a bolus of protein will elevate muscle protein synthesis to a greater extent than the consumption of an equivalent amount of protein that is consumed in small divided doses.

Study Overview

Status

Completed

Conditions

Intervention / Treatment

Detailed Description

The rapid appearance into the blood of essential amino acids, and leucine in particular, may act as an important signal to stimulate muscle protein synthesis after resistance exercise. This may explain why consuming rapidly-absorbed whey protein may have an anabolic edge over slowly-absorbed proteins such as casein. Previous investigations into importance of the rate of absorption to muscle protein synthesis that have used 'fast' and 'slow' proteins have been confounded by differences in amino acid composition. The present study addresses this issue by administering the same protein source, whey, as either a bolus or in small divided 'pulse' doses to achieve divergent amino acid profiles after a bout of resistance exercise.

This study is being conducted in young (18-35) men.

Our outcome measures include: blood amino acid concentrations, rates of myofibrillar protein synthesis, anabolic intracellular signalling markers

Study Type

Interventional

Enrollment (Actual)

8

Phase

  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

  • Canada
    • Ontario
      • Hamilton, Ontario, Canada, L8S 4K1
        • Ivor Wynne Centre A103, McMaster University

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

18 years to 35 years (Adult)

Accepts Healthy Volunteers

Yes

Genders Eligible for Study

Male

Description

Inclusion Criteria:

  • Non-obese men (BMI <27) between the age of 18 and 35 yrs.

Exclusion Criteria:

  • Type II diabetes or other known diseases
  • Use of medication
  • Female
  • Other ages or BMI than indicated above
  • Resistance training > 3X/wk

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Allocation: Randomized
  • Interventional Model: Crossover Assignment
  • Masking: None (Open Label)

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Experimental: protein feeding
Participants will complete 2 trials in a cross-over fashion in which they will consume whey protein either as a single bolus or as 10 small divided doses
Other: whey protein bolus
single dose of 25 g whey protein
Other: whey protein pulses
10 2.5 g pulses of whey protein

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Rate of myofibrillar protein synthesis
Time Frame: 4 months
Rates of myofibrillar protein synthesis will be measured from muscle biopsy samples obtained from subjects participating in the study protocol.
4 months

Secondary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Myocellular protein phosphorylation
Time Frame: 4 months
Protein phosphorylation of target proteins will be measured from Western blot analysis of muscle biopsy samples.
4 months

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

McMaster University

Collaborators

Nestec Ltd.
Australian Institute of Sport
RMIT University

Publications and helpful links

The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.

General Publications

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start

July 1, 2009

Primary Completion (Actual)

October 1, 2009

Study Completion (Actual)

October 1, 2009

Study Registration Dates

First Submitted

March 18, 2011

First Submitted That Met QC Criteria

March 18, 2011

First Posted (Estimate)

March 21, 2011

Study Record Updates

Last Update Posted (Estimate)

March 21, 2011

Last Update Submitted That Met QC Criteria

March 18, 2011

Last Verified

March 1, 2011

More Information

Terms related to this study

Other Study ID Numbers

  • Darwin 1a - Bolus versus Pulse

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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