Knee Extensor Muscle Properties in Young Women Symptomatic and Asymptomatic for Anterior Knee Pain

Knee Extensor Muscle Inhibition and Neuromechanical and Morphological Properties in Young Women Symptomatic and Asymptomatic for Anterior Knee Pain

This study aims to compare the knee extensors' mechanical, morphological and myoelectric properties between subjects affected by anterior knee pain and a healthy control group. The investigators hypothesis is that patients with anterior knee pain present a reduction in the knee extensors' mechanical, morphological and myoelectric properties due to chronic muscle inhibition produced by the syndrome. In addition, the study also aims to evaluate whether there are differences in methodology for application of the Interpolated Twitch Technique for evaluation of the quadriceps muscle inhibition by comparing the use of supramaximal electrical stimulation on the femoral nerve (gold standard) with stimulation on the motor point (new methodology) in healthy subjects and individuals affected by anterior knee pain. The investigators hypothesis is that the stimulation on the motor point is less discomfortable than on the femoral nerve and muscle inhibition results are less variable (with lower dispersion) due to such lower discomfort

Study Overview

• Status: Completed
• Conditions: Anterior Knee Pain Syndrome

Detailed Description

Participants were divided in two groups (control group, n=16 and anterior knee pain group, n=16). Prior to participation, informed consent was obtained from all subjects. The evaluation was performed at the lower limb with severe symptoms of anterior knee pain in the patients, and at the dominant limb in the control group. Quadriceps Muscle inhibition was estimated using the Interpolated Twitch Technique, which involves applying a single electrical twitch stimulation to the femoral nerve (gold standard) during a maximal isometric knee extensor contraction. Muscle inhibition measurements were obtained with the aid of a Grass S88 (Quincy, MA, USA.) Muscle Stimulator in combination with and isolation unit approved for human use. Isometric and isokinetic knee extensor torques were obtained with a Biodex System 3 dynamometer (Biodex Medical System, Shirley - NY, USA). Electromyographic muscle activity was measured with bipolar surface electrodes (Kendall, Meditrace - 100; Ag/AgCl) that were placed on the muscle bellies of vastus medialis, vastus lateralis and rectus femoris. Muscle architecture was measured with a B-mode ultrasonographic system (SSD; Aloka Inc., Tokyo, Japan) with a linear-array probe (60 mm, 7.5MHz; Aloka). Single Leg Squat was used for clinical evaluation of the lower limb functionality.

• Study Type: Observational
• Enrollment (Actual): 32

Contacts and Locations

Study Locations

• Brazil
  • Rio Grande do Sul
    • Porto Alegre, Rio Grande do Sul, Brazil, 90690-200
      • Exercise Research Laboratory, School of Physical Education, Federal University of Rio Grande do Sul

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years to 40 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Female

• Sampling Method: Probability Sample

Study Population

Young women symptomatic and assymptomatic for anterior knee pain syndrome

Description

Inclusion Criteria:

• Anterior Knee pain group:

  1. Retropatellar or peripatellar knee pain in at least 2 of the following functional activities: stair ascent or descent, running, kneeling, squatting, prolonged sitting, jumping;
  2. Score smaller than 85 points on the Kujala Questionnaire;
  3. Physically active according to the International Physical Activity Questionnaire (IPAQ - short version)
  4. Body mass index less than 30

• Control group:

  1. Knee pain-free females with no history of lower limb injuries

Exclusion Criteria:

1. Body mass index higher than 30
2. Sedentary subjects
3. Cardiovascular, neurological or cognitive disorders
4. Any cardiorespiratory, neuromuscular, or metabolic disease that could represent an absolute contraindication or a contraindication to the performance of maximum strength tests
5. Previous surgery on lower limb
6. Participation in a strength-training programme or physiotherapy treatment for knee injuries in the past six months
7. Otherwise associated injury in the lower limb joints other than anterior knee pain syndrome
8. Pregnancy

Study Plan

How is the study designed?

Number of groups / cohorts

2

Cohorts and Interventions

Group / Cohort
Control Group; Knee pain-free females with no history of lower limb injuries serving as a control group. .
Anterior Knee Pain Group; Females with anterior knee pain syndrome were enrolled in this group.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Torque	Torque is an expression of the muscular strength and was assessed by dynamometry.	Torque was measured during a single visit to the laboratory during the study.The evaluation was performed up to 5 months
Muscular architecture	Muscular architecture (muscle thickness, pennation angle and fascicle length) was assessed by ultrasonography	Muscle architecture was measured one time during the study. The evaluation was performed up to 5 months
Muscle inhibition	Quadriceps muscle inhibition was assessed using the Interpolated Twitch Technique comparing the use of supramaximal electrical stimulation on the femoral nerve (gold standard) with stimulation on the motor point (new methodology).	Muscle inhibition was measured during a single visit to the laboratory during the study. The evaluation was performed up to 5 months
Muscular electrical activation	Electrical activation was assessed by surface electromyography (EMG)	Muscular electrical activation was measured during a single visit to the laboratory during the study. The evaluation was performed up to 5 months
Patellofemoral cartilage thickness	Patellofemoral cartilage thickness were assessed by ultrasonography	Patellofemoral cartilage thickness was measured one time during the study. The evaluation was performed up to 5 months
Single Leg Squat	Single leg squat was measured with a video camera and allowed to determine lower limb functionality	Single leg squat performance was measured one time during the study. The evaluation was performed up to 5 months

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Knee pain	Knee pain was measured with a Visual Analogue Scale	Knee pain during the tests was measured after all knee extensor contractions. The evaluation was performed up to 5 months.

Collaborators and Investigators

• Sponsor: Federal University of Rio Grande do Sul

Study record dates

Study Major Dates

• Study Start: April 1, 2015
• Primary Completion (Actual): March 1, 2016
• Study Completion (Actual): October 1, 2016

Study Registration Dates

• First Submitted: January 26, 2016
• First Submitted That Met QC Criteria: April 11, 2016
• First Posted (Estimate): April 12, 2016

Study Record Updates

• Last Update Posted (Actual): October 26, 2017
• Last Update Submitted That Met QC Criteria: October 24, 2017
• Last Verified: October 1, 2017

More Information

Terms related to this study

• Keywords: Muscle Architecture; Anterior Knee Pain Syndrome; Quadriceps Femoris; Muscle Inhibition; Interpolated Twitch Technique; Single Leg Squat; Women Knee Pain
• Additional Relevant MeSH Terms: Joint Diseases; Musculoskeletal Diseases; Patellofemoral Pain Syndrome
• Other Study ID Numbers: UFRGS - 1.032.452

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO