Proteome Remodelling

Proteome Remodelling and Dynamics in Human Skeletal Muscle: Effect of Training

The purpose of the project is to investigate the proteome signature dynamics and remodelling in human skeletal muscle induced by exercise training

Study Overview

• Status: Completed
• Conditions: Skeletal Muscle Physiology
• Intervention / Treatment: Other: Endurance training; Other: Resistance training; Other: Sprint training

• Study Type: Interventional
• Enrollment (Actual): 72
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Denmark
  • Copenhagen, Denmark
    • August Krogh Building

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 45 years (Adult)
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Healthy men and women
• VO2max > 55 mL/min/kgLM for men and > 50 for women (only applicable for endurance and sprint training interventions)
• BMI < 26

Exclusion Criteria:

• Chronic disease deemed by the medical doctor to pose a risk or to affect study outcomes
• smoking
• Use of prescription medicine deemed by the medical to affect study outcomes
• Pregnancy

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Non-Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Endurance; Endurance training	Other: Endurance training; Subjects perform a period of endurance training
Experimental: Resistance; Resistance training	Other: Resistance training; Subjects perform a period of resistance training
Experimental: Sprint; Sprint training	Other: Sprint training; Subjects perform a period of sprint training

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Skeletal muscle proteome signature	Pathway enrichment analysis of the muscle proteome	Change from baseline at week 6 or 8 depending on the intervention
Anabolic signaling	Western blotting of proteins related to anabolic signaling	Change in anabolic signaling from rest to 1 hour following resistance exercise both at baseline and following 8 weeks of resistance training

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Skeletal muscle mitochondrial respiration	High resolution respirometry	Change from baseline at week 6 following endurance and sprint training
Maximal oxygen consumption	VO2max during incremental exercise	Change from baseline at week 6 following endurance and sprint training
Body composition	Lean and fat mass during dual X-ray absorptiometry	Change from baseline at week 6 or 8 depending on the intervention
Myonuclei	Immunohistochemistry to determine number of myonuclei	Change from baseline at week 8 following resistance training
Satellite cells	Immunohistochemistry to determine number of satellite cells	Change from baseline at week 8 following resistance training
Fiber size	Immunohistochemistry to determine fiber size	Change from baseline at week 8 following resistance training

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Skeletal muscle mitochondrial supercomplexes	Pathway enrichment analysis of mitochondrial supercomplexes	Change from baseline at week 6 following endurance and sprint training

Collaborators and Investigators

• Sponsor: Morten Hostrup, PhD

Study record dates

Study Major Dates

• Study Start (Actual): October 30, 2019
• Primary Completion (Actual): November 28, 2023
• Study Completion (Actual): November 28, 2023

Study Registration Dates

• First Submitted: October 21, 2019
• First Submitted That Met QC Criteria: October 21, 2019
• First Posted (Actual): October 23, 2019

Study Record Updates

• Last Update Posted (Actual): December 27, 2023
• Last Update Submitted That Met QC Criteria: December 19, 2023
• Last Verified: December 1, 2023

More Information

Terms related to this study

• Other Study ID Numbers: MiDy

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: UNDECIDED

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No