Interleukin-6 Secretion in Adipose Tissue Following Sprint Exercise

Metabolism in Adipose Tissue Followed Following Sprint Exercise

Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators will examine acute short-term exercise effects on adipose tissue IL-6 exchange.

Study Overview

• Status: Completed
• Conditions: Metabolism in Adipose Tissue
• Intervention / Treatment: Other: Repeated 30-s sprint exercise

Detailed Description

PURPOSE: Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators examined acute short-term effects on adipose tissue IL-6 exchange.

METHODS: Four female and four male subjects perform repeated sprint exercise (3 x 30s with 20 min rest between; Wingate-test). Blood samples are repeatedly obtained, up to 120 min after the last sprint (9 min post ex), from catheters inserted percutaneously into brachial artery and a superficial subcutaneous vein on the anterior abdominal wall providing access to the venous drainage from the subcutaneous adipose tissue and analyzed for IL-6 by ELISA and lactate by spectrophotometry. Fat biopsies from the stomach will be obtained before, 15 min and 120 min after the last sprint. Blood flow will be determined by administration of Xenon-133 in the adipose tissue of the stomach. The release rate of Xenon-133 will be registrated continuously during the total investigation.

SUPPORT: This study is supported by grants from the Swedish National Center for Research in Sports

• Study Type: Interventional
• Enrollment (Actual): 18
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Sweden
  • Stockholm, Sweden, 141 86
    • Karolinska Institutet/Karolinska University Hospital

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years to 40 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Physical active
• Healthy

Exclusion Criteria:

• Smokers
• Pregnant
• On medication
• No infection
• No asthma

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Other: Sprint exercise; Three bouts of 30-s sprint exercise with 20 min rest in between. Three fat biopsies obtained ar rest before first sprint, 15 min after and 120 min after third sprint Blood samples from stomach vein and arteria during the whole experiment	Other: Repeated 30-s sprint exercise; Analyze of Interleukin-6 concentration before and after repeated 30-s sprint exercise

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise	Stomach fat biopsies and arterial and stomach vein blood are obtained	Before 30-s repeated sprint exercise
Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise	Stomach fat biopsies and arterial and stomach vein blood are obtained	After 30-s repeated sprint exercise

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Changes in blood flow in adipose tissue	Xenon-133 is administered by a subcutaneous injection into the abdominal adipose tissue of approximately 1-2 MBq of Xenon-133 in the form of gas, at rest before work.	Before 30-s repeated sprint exercise
Changes in blood flow in adipose tissue	The rinsing rate of Xenon-133 from the tissue depot is continuously recorded during the study period by a non-invasive method using a portable scintillation detector system (Mediscint) up to 60 minutes after the last sprint work.	After 30-s repeated sprint exercise
Changes in different metabolites	Analysed for lactate, ammonia and IL-6	Before 30-s repeated sprint exercise
Changes in different metabolites	Analysed for lactate, ammonia and IL-6	After 30-s repeated sprint exercise

Collaborators and Investigators

• Sponsor: Karolinska Institutet

Study record dates

Study Major Dates

• Study Start: September 1, 2015
• Primary Completion (Actual): September 1, 2016
• Study Completion (Actual): September 1, 2017

Study Registration Dates

• First Submitted: September 22, 2015
• First Submitted That Met QC Criteria: September 22, 2020
• First Posted (Actual): September 28, 2020

Study Record Updates

• Last Update Posted (Actual): September 28, 2020
• Last Update Submitted That Met QC Criteria: September 22, 2020
• Last Verified: September 1, 2020

More Information

Terms related to this study

• Other Study ID Numbers: 2015/1054-32