Platelet and Immune Response in Covid-19 Vaccine (Vax-SPEED-IT)

Characterization of the Platelet and Immune Response in Adults Receiving SARS-CoV-2 Vaccine

COVID-19 mRNA vaccines, administered with a two-dose regimen, have been shown to provide protection against Covid-19. However, the thromboinflammatory response toward these vaccines has never been explored as they exploit a completely new technology. It was reported that mRNA vaccines are highly reactogenic right after vaccine administration in particular in young adults, but we do not know which cells drive the early immune response to LNP-mRNA vaccines in humans and if platelets become activated as well. Moreover, it is not known if female, who have a heightened immune response to other vaccines, are able to mount a faster response to this new type of vaccines.

Objectives of the study is to characterize the platelet and immune response and the platelet-immune cross-talk in subjects undergoing SARS-CoV-2 vaccination.

Study Overview

• Status: Recruiting
• Conditions: SARS-CoV-2 Vaccination

Detailed Description

Coronavirus disease 2019 (COVID-19) is a systemic, potentially severe and life-threatening disease, triggered by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since the first cases of SARSCoV-2 infection were officially diagnosed, in December 2019, more than 280 million cases and 5 million deaths have been declared worldwide. In response to this public health emergency the international scientific community has made an enormous effort to understand the virus and to develop a safe vaccine to prevent the spread of COVID-19. Just few days after the World Health Organization declared the SarsCoV-2 outbreak a global pandemic, it was published the structure of the Spike viral protein [1], which has provided critical clues to design vaccines. Within less than 12 months several vaccines against SARS-CoV-2 have been developed. The first vaccine that has been approved in Europe and that is already being administered in Italy is the COVID-19 RNA vaccine, developed by BioNTech-Pfizer (BNT162b2). Both this vaccine and the one developed by Moderna consist of a lipid nanoparticle (LNP) that deliver a nucleoside-modified messenger RNA (mRNA) encoding the precise genetic information of the immunogen (SARS-CoV-2 full-length spike protein) to antigen presenting cells and elicits potent immune responses. mRNA is transiently expressed, does not integrate into the genome, and is degraded by physiological pathways.mRNA vaccines are molecularly well defined, synthesized efficiently from DNA templates by in vitro transcription, which is cell- and animal-origin material-free. mRNA production and LNP formulation are fast processes of high scalability, rendering this technology suitable for rapid vaccine development and pandemic vaccine supply [2].

Both mRNA vaccines, administered with a two-dose regimen, have been shown to provide a 94-95% protection against Covid-19 in persons 16 years of age or older [3,4]. However, the thromboinflammatory response toward these vaccines has never been explored as they exploit a completely new technology. For instance, it was reported that mRNA vaccines are highly reactogenic right after vaccine administration in particular in young adults [3], but we do not know which cells drive the early immune response to LNP-mRNA vaccines in humans and if platelets become activated as well. Moreover, it is not known if female, who have a heightened immune response to other vaccines, are able to mount a faster response to this new type of vaccines.

AIMS OF THE STUDY

Primary objective To characterize the platelet and immune response and the platelet-immune cross-talk in subjects undergoing SARS-CoV-2 vaccination.

Secondary objective To study the short-term kinetics of the immune and the antibody response in subjects undergoing SARS-CoV-2 vaccination.

STUDY PROTOCOL

We will enrol female and male (1:1) volunteers without signs of infection who will be subjected to SARS-CoV-2 Vaccination.

Enrolled healthy volunteers will be asked to undergo venous blood withdrawals at the following time-points:

• T0: 0-24 hours before the first dose of the vaccine;
• T1: 72±24 hours after the first dose of vaccine administration;
• T2: 10±2 days after first dose of vaccine administration;
• T3: 0-24 hours before the second dose of vaccine;
• T4: 72±24 hours after second dose of vaccine administration;
• T5: 10±2 days after second dose of vaccine administration.

A small aliquot of blood anticoagulated with sodium citrate will be used within 1 hour from blood withdrawal to perform in vivo assays (see list below), the rest will be centrifuged within 2 hours and plasma and serum samples will be stored at -80°C for further analysis (see list below).

REFERENCES

1. Wrapp D, Wang N, Corbett KS, Goldsmith JA, Hsieh CL, Abiona O, Graham BS,McLellan JS.Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science. 2020 Mar 13;367(6483):1260-1263. doi:10.1126/science.abb2507.
2. Pardi N, Hogan MJ, Porter FW, Weissman D. mRNA vaccines - a new era in vaccinology. Nat Rev Drug Discov. 2018 Apr;17(4):261-279. doi:10.1038/nrd.2017.243.
3. Polack FP, Thomas SJ, Kitchin N, et al. Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine. N Engl J Med. 2020;383(27):2603-2615. doi:10.1056/NEJMoa2034577
4. Anderson EJ, Rouphael NG, Widge AT, Jackson LA, et al.; mRNA-1273 Study Group. Safety and Immunogenicity of SARS-CoV-2 mRNA-1273 Vaccine in Older Adults. N Engl J Med. 2020 Dec 17;383(25):2427-2438. doi: 10.1056/NEJMoa2028436.
5. Lanza GA, Barone L, Scalone G, et al.; Inflammation-related effects of adjuvant influenza A vaccination on platelet activation and cardiac autonomic function. J Intern Med. 2011 Jan;269(1):118-25. doi: 10.1111/j.1365-2796.2010.02285.x.

At baseline (T0) each adult enrolled will be asked to fill in a short questionnaire, to provide information on baseline characteristics, previous relevant medical history (i.e. chronic medical conditions, use of drugs with a particular focus on immunosuppressant agents), smoking and exposure to SARS-CoV-2 (i.e. if a previous diagnosis of COVID-19 was received).

At any further timepoint (T1-T4), each participant will be asked to fill in a short questionnaire on symptoms related to vaccination, occurrence of COVID-19 infection, and drugs used, to account for potential confounders.

• Study Type: Observational
• Enrollment (Anticipated): 32

Contacts and Locations

• Study Contact: Name: Lucia Stefanini, PhD; Phone Number: +393914747595; Email: lucia.stefanini@uniroma1.it

Study Locations

• Italy
  • Roma, Italy, 00161
    • Recruiting
    • Sapienza University of Rome
    • Contact: Lucia Stefanini, PhD; Phone Number: +3914747595; Email: lucia.stefanini@uniroma1.it

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 80 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Probability Sample

Study Population

We will enrol 32 female and male (1:1) volunteers without signs of infection who will be subjected to SARS-CoV-2 Vaccination.

Enrolled healthy volunteers will be asked to undergo venous blood withdrawals at the following time-points:

• T0: 0-24 hours before the first dose of the vaccine;
• T1: 72±24 hours after the first dose of vaccine administration;
• T2: 10±2 days after first dose of vaccine administration;
• T3: 0-24 hours before the second dose of vaccine;
• T4: 72±24 hours after second dose of vaccine administration;
• T5: 10±2 days after second dose of vaccine administration.

Description

Inclusion Criteria:

• Volunteers without signs of SARS-CoV2 infection who will be subjected to SARS-CoV-2 Vaccination

Exclusion Criteria:

• Anti-platelet or anti-coagulant medications in the past 10 days
• Autoimmune disease
• Reported severe immunosuppression
• Pregnancy or breastfeeding
• Recent transfusions of platelets or plasma

Study Plan

How is the study designed?

Design Details

• Observational Models: Cohort
• Time Perspectives: Prospective

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Platelet-leukocytes aggregates quantification	Percentage of platelet-leukocytes aggregates (PLA) among blood leukocytes. PLA are identified based on the expression of CD41a in the individual leukocyte subpopulations.	6 months

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Cytokine Array in plasma	Plasmatic inflammatory cytokine concentrations (including IL-1β, IFN-α2, IFN-γ, TNF-α, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-18, IL-23, IL-33 and MCP-1) will be analysed using commercial bead-based immunoassay.	6 months
Detection of Circulating SARS-Cov-2 Neutralization Antibodies	Serum SARS-CoV-2 neutralizing antibodies will be detected using COVID-19 Immunorank™ MICRO-ELISA (Leinco Technologies, Inc St. Louis MO).	6 months
Platelet Phenotypic and Functional Analysis	Expression of surface receptors and platelet activation in whole anticoagulated blood (in vivo assays)	6 months
Soluble markers of platelet activation: sP-selectin, sCD40L.	Plasma sP-selectin and sCD40 analysed will be analysed by commercial ELISA kits.	6 months
Pro-Inflammatory Chemokines	CCL8, MCP-1, CXCL10, CCL11, CCl17, CCL5, CCL3, CXCL9, CXCL5, CCL20, CXCL5, CCL20, CXCL1, CXCL11, CCL4 will be analysed with commercial kits.	6 months
Immunophenotyping	% of neutrophils, eosinophils, classical/nonclassical monocytes, CD4+T cells, CD8+Tcell, B cells, Treg cells, NK cells, NKT cells in whole anticoagulated blood.	6 months

Collaborators and Investigators

• Sponsor: University of Roma La Sapienza
• Collaborators: Lucia Stefanini; Stefania Basili
• Investigators: Principal Investigator: Roberto Cangemi, MD, Sapienza University of Rome; Study Chair: Stefania Basili, MD, Sapienza University of Rome

Study record dates

Study Major Dates

• Study Start (Actual): May 5, 2021
• Primary Completion (Anticipated): February 15, 2022
• Study Completion (Anticipated): April 15, 2022

Study Registration Dates

• First Submitted: December 27, 2021
• First Submitted That Met QC Criteria: December 27, 2021
• First Posted (Actual): December 29, 2021

Study Record Updates

• Last Update Posted (Actual): December 29, 2021
• Last Update Submitted That Met QC Criteria: December 27, 2021
• Last Verified: December 1, 2021

More Information

Terms related to this study

• Keywords: SARS-CoV-2; Vaccine; Covid-19; Immunity; Platelets
• Other Study ID Numbers: PLTCOVAX

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: Undecided

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No