- ICH GCP
- Registre américain des essais cliniques
- Essai clinique NCT04887883
Influence of Sex and Training on de Novo Muscle Protein Synthesis (TUT)
Influence of Sex, Acute Resistance Exercise and Training on de Novo Muscle Protein Synthesis
Aperçu de l'étude
Statut
Les conditions
Intervention / Traitement
Description détaillée
Exogenous (e.g. diet-derived) amino acids increase muscle protein synthesis and provide the building blocks for growth. While traditional infusion studies can measure the synthesis of total mixed muscle or fraction-specific protein synthetic rates, the metabolic fate of dietary amino acids can only be assessed by measuring the incorporation of a labelled amino acid (i.e. L-[1-13C]phenylalanine) into muscle protein through the oral ingestion of a intrinsically labelled food source (e.g. milk protein) . This technique has revealed in controlled laboratory settings that dietary amino acids, and not endogenous amino acids recycled from intracellular protein breakdown, may be preferentially utilized as precursors for muscle and whole body protein synthesis Therefore, it is important to characterize the incorporation of diet-derived amino acids over a 24-h post-exercise recovery period to determine how RE influences their utilization as precursors for the synthesis of new muscle proteins. The investigators are unaware of any studies that have examined the utilization of dietary amino acids for de novo muscle protein synthesis in females, highlighting an urgent need to rectify the sex-disparity in exercise-related research.
Protein requirements during resistance training have been suggested to be highest at training onset with evidence suggesting moderate daily intakes (~1.2-1.4 g·kg·d-1) can support chronic adaptations, although recent suggestions are that slightly higher intakes (~1.6 g·kg·d-1) may optimize lean mass growth. Resistance training is associated with a reduction in whole-body protein turnover but an increased net protein balance suggesting a greater efficiency of whole-body amino acid utilization with training in males , although whether this also extends to females is unknown. Acute RE and chronic training has been reported to increase intracellular amino acid recycling in the fasted state, which would be consistent with an increased amino acid efficiency. To date, however, no study has investigated whether the post-exercise incorporation of dietary amino acids into myofibrillar and sarcoplasmic proteins in a free-living setting is modified by training and/or sex.
The primary aim of the present study was to determine the dietary fate of amino acids into contractile myofibrillar and noncontractile sarcoplasmic muscle proteins after acute RE in the untrained and trained state over 24 h in a free-living setting. The investigators hypothesized that, irrespective of sex, acute RE would increase dietary amino acid incorporation in myofibrillar proteins in the untrained state with training leading to an attenuated increase suggestive of a reduced reliance on dietary amino acids in the trained state.
Type d'étude
Inscription (Réel)
Contacts et emplacements
Lieux d'étude
-
-
Ontario
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Toronto, Ontario, Canada, M9L 1C5
- Goldring Centre for High Performance Sport
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Critères de participation
Critère d'éligibilité
Âges éligibles pour étudier
Accepte les volontaires sains
Sexes éligibles pour l'étude
Méthode d'échantillonnage
Population étudiée
La description
Inclusion Criteria:
- Participants were included in the study after reporting not engaging in whole body RE or plyometrics in the past 3 months and had a BMI between 18.5-27.5 kg/m2.
- Females were included if they had a regular menstrual cycle with the last 3 months and
Exclusion Criteria:
- Participants were excluded if they: i) consumed tobacco and/or illicit anabolic drug use (e.g. testosterone, growth hormones); ii) were a vegan or had a nut allergy and; iii) participated in a study within the past year involving stable isotopes.
- Females were excluded if they used oral contraceptives and/or discontinued their use within the last 3 months.
Plan d'étude
Comment l'étude est-elle conçue ?
Détails de conception
Cohortes et interventions
Groupe / Cohorte |
Intervention / Traitement |
---|---|
Males
10 young healthy biological males aged 18 - 30 y
|
Participants performed 8 weeks of resistance training and muscle biopsies were taken before and 24 h before and after the program.
|
Females
10 young healthy biological females aged 18 - 30 y
|
Participants performed 8 weeks of resistance training and muscle biopsies were taken before and 24 h before and after the program.
|
Que mesure l'étude ?
Principaux critères de jugement
Mesure des résultats |
Description de la mesure |
Délai |
---|---|---|
Dietary fate of amino acids into myofibrillar and sarcoplasmic proteins
Délai: The change in muscle MPE at 0 and 24 h after acute resistance exercise before and after training
|
MPE
|
The change in muscle MPE at 0 and 24 h after acute resistance exercise before and after training
|
Mesures de résultats secondaires
Mesure des résultats |
Description de la mesure |
Délai |
---|---|---|
Amino acid transporter protein content
Délai: 0 and 24 hours after acute resistance exercise before and after training
|
Arbitrary units
|
0 and 24 hours after acute resistance exercise before and after training
|
mTOR localization with capillaries
Délai: 0 and 24 hours after acute resistance exercise before and after training
|
Pearson's r
|
0 and 24 hours after acute resistance exercise before and after training
|
Collaborateurs et enquêteurs
Parrainer
Collaborateurs
Les enquêteurs
- Chercheur principal: Daniel Moore, PhD, University of Toronto
Publications et liens utiles
Dates d'enregistrement des études
Dates principales de l'étude
Début de l'étude (Réel)
Achèvement primaire (Réel)
Achèvement de l'étude (Réel)
Dates d'inscription aux études
Première soumission
Première soumission répondant aux critères de contrôle qualité
Première publication (Réel)
Mises à jour des dossiers d'étude
Dernière mise à jour publiée (Réel)
Dernière mise à jour soumise répondant aux critères de contrôle qualité
Dernière vérification
Plus d'information
Termes liés à cette étude
Autres numéros d'identification d'étude
- TUT study
- RGPIN-2015-04521 (Autre subvention/numéro de financement: NSERC Discovery Grant)
Plan pour les données individuelles des participants (IPD)
Prévoyez-vous de partager les données individuelles des participants (DPI) ?
Informations sur les médicaments et les dispositifs, documents d'étude
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