Influence of Sex and Training on de Novo Muscle Protein Synthesis (TUT)
Influence of Sex, Acute Resistance Exercise and Training on de Novo Muscle Protein Synthesis
研究概览
详细说明
Exogenous (e.g. diet-derived) amino acids increase muscle protein synthesis and provide the building blocks for growth. While traditional infusion studies can measure the synthesis of total mixed muscle or fraction-specific protein synthetic rates, the metabolic fate of dietary amino acids can only be assessed by measuring the incorporation of a labelled amino acid (i.e. L-[1-13C]phenylalanine) into muscle protein through the oral ingestion of a intrinsically labelled food source (e.g. milk protein) . This technique has revealed in controlled laboratory settings that dietary amino acids, and not endogenous amino acids recycled from intracellular protein breakdown, may be preferentially utilized as precursors for muscle and whole body protein synthesis Therefore, it is important to characterize the incorporation of diet-derived amino acids over a 24-h post-exercise recovery period to determine how RE influences their utilization as precursors for the synthesis of new muscle proteins. The investigators are unaware of any studies that have examined the utilization of dietary amino acids for de novo muscle protein synthesis in females, highlighting an urgent need to rectify the sex-disparity in exercise-related research.
Protein requirements during resistance training have been suggested to be highest at training onset with evidence suggesting moderate daily intakes (~1.2-1.4 g·kg·d-1) can support chronic adaptations, although recent suggestions are that slightly higher intakes (~1.6 g·kg·d-1) may optimize lean mass growth. Resistance training is associated with a reduction in whole-body protein turnover but an increased net protein balance suggesting a greater efficiency of whole-body amino acid utilization with training in males , although whether this also extends to females is unknown. Acute RE and chronic training has been reported to increase intracellular amino acid recycling in the fasted state, which would be consistent with an increased amino acid efficiency. To date, however, no study has investigated whether the post-exercise incorporation of dietary amino acids into myofibrillar and sarcoplasmic proteins in a free-living setting is modified by training and/or sex.
The primary aim of the present study was to determine the dietary fate of amino acids into contractile myofibrillar and noncontractile sarcoplasmic muscle proteins after acute RE in the untrained and trained state over 24 h in a free-living setting. The investigators hypothesized that, irrespective of sex, acute RE would increase dietary amino acid incorporation in myofibrillar proteins in the untrained state with training leading to an attenuated increase suggestive of a reduced reliance on dietary amino acids in the trained state.
研究类型
注册 (实际的)
联系人和位置
学习地点
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Ontario
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Toronto、Ontario、加拿大、M9L 1C5
- Goldring Centre for High Performance Sport
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参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
取样方法
研究人群
描述
Inclusion Criteria:
- Participants were included in the study after reporting not engaging in whole body RE or plyometrics in the past 3 months and had a BMI between 18.5-27.5 kg/m2.
- Females were included if they had a regular menstrual cycle with the last 3 months and
Exclusion Criteria:
- Participants were excluded if they: i) consumed tobacco and/or illicit anabolic drug use (e.g. testosterone, growth hormones); ii) were a vegan or had a nut allergy and; iii) participated in a study within the past year involving stable isotopes.
- Females were excluded if they used oral contraceptives and/or discontinued their use within the last 3 months.
学习计划
研究是如何设计的?
设计细节
队列和干预
团体/队列 |
干预/治疗 |
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Males
10 young healthy biological males aged 18 - 30 y
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Participants performed 8 weeks of resistance training and muscle biopsies were taken before and 24 h before and after the program.
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Females
10 young healthy biological females aged 18 - 30 y
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Participants performed 8 weeks of resistance training and muscle biopsies were taken before and 24 h before and after the program.
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研究衡量的是什么?
主要结果指标
结果测量 |
措施说明 |
大体时间 |
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Dietary fate of amino acids into myofibrillar and sarcoplasmic proteins
大体时间:The change in muscle MPE at 0 and 24 h after acute resistance exercise before and after training
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MPE
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The change in muscle MPE at 0 and 24 h after acute resistance exercise before and after training
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次要结果测量
结果测量 |
措施说明 |
大体时间 |
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Amino acid transporter protein content
大体时间:0 and 24 hours after acute resistance exercise before and after training
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Arbitrary units
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0 and 24 hours after acute resistance exercise before and after training
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mTOR localization with capillaries
大体时间:0 and 24 hours after acute resistance exercise before and after training
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Pearson's r
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0 and 24 hours after acute resistance exercise before and after training
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合作者和调查者
调查人员
- 首席研究员:Daniel Moore, PhD、University of Toronto
出版物和有用的链接
研究记录日期
研究主要日期
学习开始 (实际的)
初级完成 (实际的)
研究完成 (实际的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (实际的)
研究记录更新
最后更新发布 (实际的)
上次提交的符合 QC 标准的更新
最后验证
更多信息
与本研究相关的术语
其他研究编号
- TUT study
- RGPIN-2015-04521 (其他赠款/资助编号:NSERC Discovery Grant)
计划个人参与者数据 (IPD)
计划共享个人参与者数据 (IPD)?
药物和器械信息、研究文件
研究美国 FDA 监管的药品
研究美国 FDA 监管的设备产品
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Resistance exercise的临床试验
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University of Maryland, BaltimoreNational Institute on Aging (NIA)完全的