- ICH GCP
- Amerikanska kliniska prövningsregistret
- Klinisk prövning NCT04887883
Influence of Sex and Training on de Novo Muscle Protein Synthesis (TUT)
Influence of Sex, Acute Resistance Exercise and Training on de Novo Muscle Protein Synthesis
Studieöversikt
Status
Betingelser
Intervention / Behandling
Detaljerad beskrivning
Exogenous (e.g. diet-derived) amino acids increase muscle protein synthesis and provide the building blocks for growth. While traditional infusion studies can measure the synthesis of total mixed muscle or fraction-specific protein synthetic rates, the metabolic fate of dietary amino acids can only be assessed by measuring the incorporation of a labelled amino acid (i.e. L-[1-13C]phenylalanine) into muscle protein through the oral ingestion of a intrinsically labelled food source (e.g. milk protein) . This technique has revealed in controlled laboratory settings that dietary amino acids, and not endogenous amino acids recycled from intracellular protein breakdown, may be preferentially utilized as precursors for muscle and whole body protein synthesis Therefore, it is important to characterize the incorporation of diet-derived amino acids over a 24-h post-exercise recovery period to determine how RE influences their utilization as precursors for the synthesis of new muscle proteins. The investigators are unaware of any studies that have examined the utilization of dietary amino acids for de novo muscle protein synthesis in females, highlighting an urgent need to rectify the sex-disparity in exercise-related research.
Protein requirements during resistance training have been suggested to be highest at training onset with evidence suggesting moderate daily intakes (~1.2-1.4 g·kg·d-1) can support chronic adaptations, although recent suggestions are that slightly higher intakes (~1.6 g·kg·d-1) may optimize lean mass growth. Resistance training is associated with a reduction in whole-body protein turnover but an increased net protein balance suggesting a greater efficiency of whole-body amino acid utilization with training in males , although whether this also extends to females is unknown. Acute RE and chronic training has been reported to increase intracellular amino acid recycling in the fasted state, which would be consistent with an increased amino acid efficiency. To date, however, no study has investigated whether the post-exercise incorporation of dietary amino acids into myofibrillar and sarcoplasmic proteins in a free-living setting is modified by training and/or sex.
The primary aim of the present study was to determine the dietary fate of amino acids into contractile myofibrillar and noncontractile sarcoplasmic muscle proteins after acute RE in the untrained and trained state over 24 h in a free-living setting. The investigators hypothesized that, irrespective of sex, acute RE would increase dietary amino acid incorporation in myofibrillar proteins in the untrained state with training leading to an attenuated increase suggestive of a reduced reliance on dietary amino acids in the trained state.
Studietyp
Inskrivning (Faktisk)
Kontakter och platser
Studieorter
-
-
Ontario
-
Toronto, Ontario, Kanada, M9L 1C5
- Goldring Centre for High Performance Sport
-
-
Deltagandekriterier
Urvalskriterier
Åldrar som är berättigade till studier
Tar emot friska volontärer
Kön som är behöriga för studier
Testmetod
Studera befolkning
Beskrivning
Inclusion Criteria:
- Participants were included in the study after reporting not engaging in whole body RE or plyometrics in the past 3 months and had a BMI between 18.5-27.5 kg/m2.
- Females were included if they had a regular menstrual cycle with the last 3 months and
Exclusion Criteria:
- Participants were excluded if they: i) consumed tobacco and/or illicit anabolic drug use (e.g. testosterone, growth hormones); ii) were a vegan or had a nut allergy and; iii) participated in a study within the past year involving stable isotopes.
- Females were excluded if they used oral contraceptives and/or discontinued their use within the last 3 months.
Studieplan
Hur är studien utformad?
Designdetaljer
Kohorter och interventioner
Grupp / Kohort |
Intervention / Behandling |
---|---|
Males
10 young healthy biological males aged 18 - 30 y
|
Participants performed 8 weeks of resistance training and muscle biopsies were taken before and 24 h before and after the program.
|
Females
10 young healthy biological females aged 18 - 30 y
|
Participants performed 8 weeks of resistance training and muscle biopsies were taken before and 24 h before and after the program.
|
Vad mäter studien?
Primära resultatmått
Resultatmått |
Åtgärdsbeskrivning |
Tidsram |
---|---|---|
Dietary fate of amino acids into myofibrillar and sarcoplasmic proteins
Tidsram: The change in muscle MPE at 0 and 24 h after acute resistance exercise before and after training
|
MPE
|
The change in muscle MPE at 0 and 24 h after acute resistance exercise before and after training
|
Sekundära resultatmått
Resultatmått |
Åtgärdsbeskrivning |
Tidsram |
---|---|---|
Amino acid transporter protein content
Tidsram: 0 and 24 hours after acute resistance exercise before and after training
|
Arbitrary units
|
0 and 24 hours after acute resistance exercise before and after training
|
mTOR localization with capillaries
Tidsram: 0 and 24 hours after acute resistance exercise before and after training
|
Pearson's r
|
0 and 24 hours after acute resistance exercise before and after training
|
Samarbetspartners och utredare
Sponsor
Samarbetspartners
Utredare
- Huvudutredare: Daniel Moore, PhD, University of Toronto
Publikationer och användbara länkar
Studieavstämningsdatum
Studera stora datum
Studiestart (Faktisk)
Primärt slutförande (Faktisk)
Avslutad studie (Faktisk)
Studieregistreringsdatum
Först inskickad
Först inskickad som uppfyllde QC-kriterierna
Första postat (Faktisk)
Uppdateringar av studier
Senaste uppdatering publicerad (Faktisk)
Senaste inskickade uppdateringen som uppfyllde QC-kriterierna
Senast verifierad
Mer information
Termer relaterade till denna studie
Andra studie-ID-nummer
- TUT study
- RGPIN-2015-04521 (Annat bidrag/finansieringsnummer: NSERC Discovery Grant)
Plan för individuella deltagardata (IPD)
Planerar du att dela individuella deltagardata (IPD)?
Läkemedels- och apparatinformation, studiedokument
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