- ICH GCP
- 미국 임상 시험 레지스트리
- 임상시험 NCT04887883
Influence of Sex and Training on de Novo Muscle Protein Synthesis (TUT)
Influence of Sex, Acute Resistance Exercise and Training on de Novo Muscle Protein Synthesis
연구 개요
상세 설명
Exogenous (e.g. diet-derived) amino acids increase muscle protein synthesis and provide the building blocks for growth. While traditional infusion studies can measure the synthesis of total mixed muscle or fraction-specific protein synthetic rates, the metabolic fate of dietary amino acids can only be assessed by measuring the incorporation of a labelled amino acid (i.e. L-[1-13C]phenylalanine) into muscle protein through the oral ingestion of a intrinsically labelled food source (e.g. milk protein) . This technique has revealed in controlled laboratory settings that dietary amino acids, and not endogenous amino acids recycled from intracellular protein breakdown, may be preferentially utilized as precursors for muscle and whole body protein synthesis Therefore, it is important to characterize the incorporation of diet-derived amino acids over a 24-h post-exercise recovery period to determine how RE influences their utilization as precursors for the synthesis of new muscle proteins. The investigators are unaware of any studies that have examined the utilization of dietary amino acids for de novo muscle protein synthesis in females, highlighting an urgent need to rectify the sex-disparity in exercise-related research.
Protein requirements during resistance training have been suggested to be highest at training onset with evidence suggesting moderate daily intakes (~1.2-1.4 g·kg·d-1) can support chronic adaptations, although recent suggestions are that slightly higher intakes (~1.6 g·kg·d-1) may optimize lean mass growth. Resistance training is associated with a reduction in whole-body protein turnover but an increased net protein balance suggesting a greater efficiency of whole-body amino acid utilization with training in males , although whether this also extends to females is unknown. Acute RE and chronic training has been reported to increase intracellular amino acid recycling in the fasted state, which would be consistent with an increased amino acid efficiency. To date, however, no study has investigated whether the post-exercise incorporation of dietary amino acids into myofibrillar and sarcoplasmic proteins in a free-living setting is modified by training and/or sex.
The primary aim of the present study was to determine the dietary fate of amino acids into contractile myofibrillar and noncontractile sarcoplasmic muscle proteins after acute RE in the untrained and trained state over 24 h in a free-living setting. The investigators hypothesized that, irrespective of sex, acute RE would increase dietary amino acid incorporation in myofibrillar proteins in the untrained state with training leading to an attenuated increase suggestive of a reduced reliance on dietary amino acids in the trained state.
연구 유형
등록 (실제)
연락처 및 위치
연구 장소
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Ontario
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Toronto, Ontario, 캐나다, M9L 1C5
- Goldring Centre for High Performance Sport
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참여기준
자격 기준
공부할 수 있는 나이
건강한 자원 봉사자를 받아들입니다
연구 대상 성별
샘플링 방법
연구 인구
설명
Inclusion Criteria:
- Participants were included in the study after reporting not engaging in whole body RE or plyometrics in the past 3 months and had a BMI between 18.5-27.5 kg/m2.
- Females were included if they had a regular menstrual cycle with the last 3 months and
Exclusion Criteria:
- Participants were excluded if they: i) consumed tobacco and/or illicit anabolic drug use (e.g. testosterone, growth hormones); ii) were a vegan or had a nut allergy and; iii) participated in a study within the past year involving stable isotopes.
- Females were excluded if they used oral contraceptives and/or discontinued their use within the last 3 months.
공부 계획
연구는 어떻게 설계됩니까?
디자인 세부사항
코호트 및 개입
그룹/코호트 |
개입 / 치료 |
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Males
10 young healthy biological males aged 18 - 30 y
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Participants performed 8 weeks of resistance training and muscle biopsies were taken before and 24 h before and after the program.
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Females
10 young healthy biological females aged 18 - 30 y
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Participants performed 8 weeks of resistance training and muscle biopsies were taken before and 24 h before and after the program.
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연구는 무엇을 측정합니까?
주요 결과 측정
결과 측정 |
측정값 설명 |
기간 |
---|---|---|
Dietary fate of amino acids into myofibrillar and sarcoplasmic proteins
기간: The change in muscle MPE at 0 and 24 h after acute resistance exercise before and after training
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MPE
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The change in muscle MPE at 0 and 24 h after acute resistance exercise before and after training
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2차 결과 측정
결과 측정 |
측정값 설명 |
기간 |
---|---|---|
Amino acid transporter protein content
기간: 0 and 24 hours after acute resistance exercise before and after training
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Arbitrary units
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0 and 24 hours after acute resistance exercise before and after training
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mTOR localization with capillaries
기간: 0 and 24 hours after acute resistance exercise before and after training
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Pearson's r
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0 and 24 hours after acute resistance exercise before and after training
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공동 작업자 및 조사자
수사관
- 수석 연구원: Daniel Moore, PhD, University of Toronto
간행물 및 유용한 링크
연구 기록 날짜
연구 주요 날짜
연구 시작 (실제)
기본 완료 (실제)
연구 완료 (실제)
연구 등록 날짜
최초 제출
QC 기준을 충족하는 최초 제출
처음 게시됨 (실제)
연구 기록 업데이트
마지막 업데이트 게시됨 (실제)
QC 기준을 충족하는 마지막 업데이트 제출
마지막으로 확인됨
추가 정보
이 연구와 관련된 용어
기타 연구 ID 번호
- TUT study
- RGPIN-2015-04521 (기타 보조금/기금 번호: NSERC Discovery Grant)
개별 참가자 데이터(IPD) 계획
개별 참가자 데이터(IPD)를 공유할 계획입니까?
약물 및 장치 정보, 연구 문서
미국 FDA 규제 의약품 연구
미국 FDA 규제 기기 제품 연구
이 정보는 변경 없이 clinicaltrials.gov 웹사이트에서 직접 가져온 것입니다. 귀하의 연구 세부 정보를 변경, 제거 또는 업데이트하도록 요청하는 경우 register@clinicaltrials.gov. 문의하십시오. 변경 사항이 clinicaltrials.gov에 구현되는 즉시 저희 웹사이트에도 자동으로 업데이트됩니다. .
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