Evaluating the Diagnostic Validity of Inflammation-associated Markers for Tuberculous Pleurisy
- To investigate the difference of PE inflammation/apoptosis-associated markers between TB pleurisy and non-TB pleurisy
- To investigate the difference of neutrophil apoptosis in exudative PE between TB pleurisy and non-TB pleurisy
- To investigate the change of apoptosis pattern of PE neutrophil, before and after TB antigen stimulation, and compare the difference between TB pleurisy and non-TB pleurisy
- To investigate diagnostic aid of the inflammation/apoptosis-associated markers and apoptosis pattern of PE neutrophil for tuberculous pleurisy
研究概览
详细说明
Tuberculosis (TB) remains a global health problem even though it has nearly been eradicated in some developed countries. Because of variable manifestations and the difficulty in collecting clinical samples, extra-pulmonary TB is usually difficult to early diagnose. Tuberculous pleurisy (TP) is one of the most common extra-pulmonary infection and accounts for approximately 5% of all forms of TB. The gold standard for diagnosing TP is mycobacterial culture of pleural effusion (PE), pleura tissue, which requires weeks to yield. The treatment could thus be delayed, resulting in an increased mortality rate. In addition, mycobacterial culture is not so sensitive for PE and with positivity in less than two thirds of cases with TB pleurisy.
For diagnosing TP, PE biomarkers are required to be investigated in addition to traditional PE cell counting and biochemistry. In particularly, inflammation-associated cytokines and apoptosis-associated markers may be important because the two pathways involve in TB infection/defense mechanism. For inflammation-association markers, current literature is not comprehensive except IFN-gamma and IFN-gamma release assay (IGRA). However, the result of IGRA using PE is disappointed. We should study other PE inflammation markers such as IFN-induced protein-10, interleukin [IL]-2, IL-12 and so on. On the other hand, apoptosis suppression is one of escape mechanisms in TB pathogenesis. Macrophage, dendritic cell, and neutrophil are reportedly inhibited for apoptosis in TB infection. But the apoptosis of PE neutrophil are rarely studied. Moreover, the role of apoptosis-associated markers (Fas ligand [FasL], decoy receptor 3, lipoxin, prostaglandin E2, caspases) in PE has rarely been investigated in diagnosing TP except FasL. Therefore, we conduct a prospective study to investigate inflammation/apoptosis-associated markers in exudative PE and apoptosis of PE neutrophil to analyze their diagnostic usefulness for TP.
研究类型
注册 (预期的)
联系人和位置
学习联系方式
- 姓名:Chin-Chung Shu, MD
- 电话号码:886-972653087
- 邮箱:ccshu139@ntu.edu.tw
学习地点
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Taipei、台湾、100
- 招聘中
- National Taiwan University Hospital
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首席研究员:
- Chin-Chung Shu, MD
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接触:
- Chin-Chung Shu, MD
- 电话号码:886972653087
- 邮箱:ccshu139@ntu.edu.tw
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参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
取样方法
研究人群
- Patients with tuberculous pleural effusion
- Patients with pleural effusion due to causes other than tuberculosis
描述
Inclusion Criteria:
- patient older than 20 years old
- patients with exudative pleural effusion
Exclusion Criteria:
- refusal of enrollment
学习计划
研究是如何设计的?
设计细节
队列和干预
团体/队列 |
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Group with tuberculous pleurisy
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Group with non-tuberculous pleurisy
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研究衡量的是什么?
主要结果指标
结果测量 |
大体时间 |
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diagnosis of tuberculous pleurisy
大体时间:2 years
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2 years
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次要结果测量
结果测量 |
大体时间 |
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死亡
大体时间:2年
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2年
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合作者和调查者
调查人员
- 首席研究员:Chin-Chung Shu, MD、National Taiwan University Hospital
研究记录日期
研究主要日期
学习开始
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (估计)
研究记录更新
最后更新发布 (估计)
上次提交的符合 QC 标准的更新
最后验证
更多信息
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