Evaluation of Skeletal Muscle Lipid Content by Magnetic Resonance Spectroscopy

Evaluation of Intramyocellular Lipid Content (IMCL) in Skeletal Muscle by Magnetic Resonance Spectroscopy

Type 2 diabetes is a growing public health issue and one component of Type 2 diabetes is 'insulin resistance" - the ability of insulin to work less well. The skeletal muscle is one of the largest users of sugar in the body, when the skeletal muscle is "insulin resistant", this contributes to diabetes. There is some evidence that the amount of fat in the skeletal muscle is related to the amount of insulin resistance. We are asking people to participate in a study to look at the amount of fat in muscle and how this fat amount changes with exercise. We hypothesize that heavier people will have higher amounts of fat and deplete this fat more slowly than lean subjects.

Study Overview

• Status: Withdrawn
• Conditions: Healthy

Detailed Description

High intramyocellular lipid (IMCL) content in skeletal muscle has been correlated to insulin resistance in obese subjects. Yet, endurance athletes have high intramyocellular lipid content and very low insulin resistance. There are several explanations for this paradox. This study will use a new magnetic resonance imaging technique to look at the amount of fat in the muscle and its depletion with exercise. We hypothesize that overweight subjects will have higher amounts of fat and deplete this fat more slowly than lean subjects.

• Study Type: Observational

Contacts and Locations

Study Locations

• United States
  • Minnesota
    • Minneapolis, Minnesota, United States, 55455
      • Univeresity of Minnesota

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 45 years (ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

Healthy subjects on regular running program.

Description

Inclusion Criteria:

• Age range will be from age 18 to 45. We will be limiting the upper age range because increased age is associated with sarcopenia and alteration of fiber type.
• We will define the lean trained group by a BMI of < 24 kg/m2. We will define the overweight group as BMI > 26 25 kg/m2.
• The subjects should be participating in regular running exercise (> 30 min/day, ≥ 5 days/week).
• Weight must be stable [+/- 5 pounds] for at least the three months prior to the study for all participants.

Exclusion Criteria:

• The subjects must not be on medications that may affect lipid levels, specifically lipid lowering agents, birth control pills, or diuretics.
• The subjects should not be on a high fat diet (> 45% fat) as measured by a screening questionnaire.
• The subject must not have any contraindications to magnetic resonance imaging.

Study Plan

How is the study designed?

Number of groups / cohorts

2

Cohorts and Interventions

Group / Cohort
1; overweight runners (BMI>25)
2; lean runners (BMI<24)

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
absolute change in intramyocellular lipid quantity with exercise	over 4 months

Collaborators and Investigators

• Sponsor: University of Minnesota
• Collaborators: National Center for Research Resources (NCRR)
• Investigators: Principal Investigator: Lisa S Chow, University of Minnesota

Study record dates

Study Major Dates

• Study Start: January 1, 2010
• Primary Completion (ACTUAL): January 1, 2014
• Study Completion (ACTUAL): January 1, 2014

Study Registration Dates

• First Submitted: November 5, 2008
• First Submitted That Met QC Criteria: November 5, 2008
• First Posted (ESTIMATE): November 6, 2008

Study Record Updates

• Last Update Posted (ESTIMATE): October 24, 2014
• Last Update Submitted That Met QC Criteria: October 23, 2014
• Last Verified: October 1, 2014

More Information

Terms related to this study

• Keywords: insulin resistance; training; intramyocellular lipid
• Other Study ID Numbers: 0708M13941; 5K12RR023247-02 (NIH)