Metabolic Consequences of Moderate Weight Gain - Role of Dietary Fat Composition (LIPOGAIN) (LIPOGAIN)

Metabolic Consequences of Moderate Weight Gain - Role of Dietary Fat Composition (LIPOGAIN): a Randomized Double-Blind Controlled Study

The purpose of this study is to investigate the metabolic consequences of a moderate weight gain and if the type of dietary fat (saturated versus polyunsaturated) can modify the effects in young healthy adults. Hypothesis: the type of dietary fat can modify the effects of weight gain.

Study Overview

• Status: Completed
• Conditions: Healthy
• Intervention / Treatment: Other: Saturated fatty acid group; Other: Polyunsaturated fatty acid group

Detailed Description

Specific goals:

• Investigate if dietary fat composition influences liver fat accumulation and metabolic risk factors in response to moderate weight gain
• Investigate if polyunsaturated fat (PUFA) in the diet could counteract any unfavorable metabolic changes that are expected to accompany moderate weight gain
• Investigate effects of weight gain and dietary fat composition on markers of vascular health

• Study Type: Interventional
• Enrollment (Actual): 41
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Sweden
  • Uppsala, Sweden
    • Uppsala University Hospital, Uppsala Science Park

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years to 38 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Healthy,
• Body Mass Index 18-27

Exclusion Criteria:

• Liver disease,
• Coronary heart disease,
• Diabetes mellitus,
• Malignant diseases,
• Alcohol or drug abuse,
• Magnetic Resonance-incompatibility,
• Abnormal clinical chemistry,
• Use of drugs that significantly effects energy metabolism,
• Heavy exercise,
• Pregnancy or lactation,
• Allergy of gluten, egg or milk protein

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Double

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Active Comparator: Saturated fatty acid group; Addition of saturated fatty acids to the diet inte the form of pastries	Other: Saturated fatty acid group; Addition of saturated fatty acids to the diet in the form of pastries
Active Comparator: Polyunsaturated fatty acid group; Addition of polyunsaturated fatty acids to the diet in the form of pastries	Other: Polyunsaturated fatty acid group; Addition of polyunsaturated fatty acids to the diet in the form of pastries

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Hepatic steatosis by magnetic resonance tomography (MRT)	6 weeks

Secondary Outcome Measures

Outcome Measure	Time Frame
Cholesterol	6 weeks
Triglycerides	6 weeks
Insulin	6 weeks
Glucose	6 weeks
high density lipoprotein (HDL)	6 weeks
low density lipoprotein (LDL)	6 weeks
Apolipoprotein B (ApoB)	6 weeks
Apolipoprotein A1 (ApoA1)	6 weeks
Aspartate aminotransferase (ASAT)	6 weeks
Alanine aminotransferase (ALAT)	6 weeks
Gamma glutamyl transpeptidase (GGT)	6 weeks
Homeostasis model of assessment insulin resistance (HOMA-IR)	6 weeks
C-reactive protein (CRP)	6 weeks
Proinsulin	6 weeks
Endostatin	6 weeks
Pentraxin-3 (PTX-3)	6 weeks
Endothelin-1 (ET-1)	6 weeks
E-selectin	6 weeks
Cathepsin-S	6 weeks
Cystatin C	6 weeks

Collaborators and Investigators

• Sponsor: Uppsala University
• Investigators: Principal Investigator: Ulf Risérus, Ass. professor, Clinical Nutrition and Metabolism, Dept. of Public Health and Caring Sciences, Uppsala University

Publications and helpful links

General Publications

• Rosqvist F, Orho-Melander M, Kullberg J, Iggman D, Johansson HE, Cedernaes J, Ahlstrom H, Riserus U. Abdominal Fat and Metabolic Health Markers but Not PNPLA3 Genotype Predicts Liver Fat Accumulation in Response to Excess Intake of Energy and Saturated Fat in Healthy Individuals. Front Nutr. 2020 Dec 3;7:606004. doi: 10.3389/fnut.2020.606004. eCollection 2020.
• Perfilyev A, Dahlman I, Gillberg L, Rosqvist F, Iggman D, Volkov P, Nilsson E, Riserus U, Ling C. Impact of polyunsaturated and saturated fat overfeeding on the DNA-methylation pattern in human adipose tissue: a randomized controlled trial. Am J Clin Nutr. 2017 Apr;105(4):991-1000. doi: 10.3945/ajcn.116.143164. Epub 2017 Mar 8. Erratum In: Am J Clin Nutr. 2017 Jul;106(1):325.
• Iggman D, Rosqvist F, Larsson A, Arnlov J, Beckman L, Rudling M, Riserus U. Role of dietary fats in modulating cardiometabolic risk during moderate weight gain: a randomized double-blind overfeeding trial (LIPOGAIN study). J Am Heart Assoc. 2014 Oct 15;3(5):e001095. doi: 10.1161/JAHA.114.001095.

Study record dates

Study Major Dates

• Study Start: September 1, 2011
• Primary Completion (Actual): December 1, 2011
• Study Completion (Actual): December 1, 2011

Study Registration Dates

• First Submitted: August 19, 2011
• First Submitted That Met QC Criteria: August 31, 2011
• First Posted (Estimate): September 1, 2011

Study Record Updates

• Last Update Posted (Estimate): December 9, 2011
• Last Update Submitted That Met QC Criteria: December 8, 2011
• Last Verified: December 1, 2011

More Information

Terms related to this study

• Keywords: Weight gain; Sunflower oil; Dietary fatty acids; Liver fat; Polyunsaturated fatty acids (PUFA); Saturated fatty acids (SFA); Overfeed; Palm oil
• Additional Relevant MeSH Terms: Body Weight; Body Weight Changes; Weight Gain
• Other Study ID Numbers: SLS-2011-LPG