P. Gingivalis IgG Titer Test for Periodontitis

Assessment of Use of Plasma or Serum IgG Test to Screen for Periodontitis

This study was performed to evaluate prospectively the diagnostic utility of a blood IgG antibody titer test against periodontal pathogens.

Study Overview

• Status: Completed
• Conditions: Periodontitis

Detailed Description

Background: Periodontitis is a prevalent silent infectious disease worldwide, and affects lifestyle-related diseases such as diabetes. Therefore, efficient screening of patients is essential for general health. This study was performed to evaluate prospectively the diagnostic utility of a blood IgG antibody titer test against periodontal pathogens.

Methods: Oral examination was performed and blood IgG antibody titers against periodontal pathogens were measured by ELISA, in 1387 subjects. The cut-off value of IgG titer was determined in receiver operating characteristic curve analysis, and changes in periodontal clinical parameters and IgG titers by periodontal treatment were evaluated. The relationships between IgG titers and severity of periodontitis were analyzed.

• Study Type: Observational
• Enrollment (Actual): 1387

Contacts and Locations

Study Locations

• Japan
  • Hiroshima, Japan, 7348553
    • Hiroshima University Graduate School of Biomedical Sciences
  • Nagasaki, Japan, 8528588
    • Nagasaki University Graduate School of Biomedical Sciences
  • Niigata, Japan, 9518514
    • Niigata University Faculty of Dentistry
  • Okayama, Japan, 7008525
    • Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
  • Tokushima, Japan, 7708504
    • The University of Tokushima Graduate School, Institute of Health Biosciences
  • Aichi
    • Nagoya, Aichi, Japan, 4648650
      • Aichi Gakuin University, School of Dentistry
    • Toyota, Aichi, Japan, 4442225
      • Toyota Motor Corporation
  • Chiba
    • Matsudo, Chiba, Japan, 2718587
      • Nihon University School of Dentistry at Matsudo
  • Miyagi
    • Sendai, Miyagi, Japan, 9808575
      • Tohoku University Graduate School of Dentistry
  • Osaka
    • Suita, Osaka, Japan, 5650871
      • Osaka University Graduate School of Dentistry
  • Tokyo
    • Bunkyo-ku, Tokyo, Japan, 1138510
      • Tokyo Medical and Dental University Graduate School
    • Chiyoda-ku, Tokyo, Japan, 1020071
      • Nippon Dental University, School of Life Dentistry at Tokyo

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

Patients who visit university hospitals to receive periodontal treatment are patients group.

Employees who receive occupational health check at a company facility.

Description

Inclusion Criteria:

• periodontal patients
• adults

Exclusion Criteria:

• difficulty of communication
• received periodontal therapy within 3 months
• with other diseases, i.e., hypertension, diabetes (self reported)

Study Plan

How is the study designed?

Number of groups / cohorts

1

Cohorts and Interventions

Group / Cohort
periodontitis; those with periodontitis and those without periodontitis

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Level of IgG antibodies in serum/plasma against periodontal pathogens	Serum/plasma samples will be screened for the presence of IgG antibodies against the below mentioned periodontal pathogens, using the "ELISA" method. Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Prevotella intermedius, Porphyromonas gingivalis	1 to 3 months after basic periodontal treatment (Scaling and root planing)

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Periodontal pocket depth	Clinical observation on periodontal pocket depth before and after basic periodontal treatment, in order to confirm appropriate effects of the treatment.	1 to 3 months after basic periodontal treatment
Bleeding on probing	Judging from bleeding from gingiva after periodontal pocket probing, degree of inflammation will be understood.	1 to 3 monthsafter basic periodontal treatment
Tooth mobility	Clinical observation to understand decrease of inflammation	1 to 3 months after basic periodontal treatment

Collaborators and Investigators

• Sponsor: Okayama University
• Investigators: Principal Investigator: Shogo Takashiba, Professor, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Department of Pathiphysiology - Periodontal Science

Publications and helpful links

General Publications

• Kudo C, Naruishi K, Maeda H, Abiko Y, Hino T, Iwata M, Mitsuhashi C, Murakami S, Nagasawa T, Nagata T, Yoneda S, Nomura Y, Noguchi T, Numabe Y, Ogata Y, Sato T, Shimauchi H, Yamazaki K, Yoshimura A, Takashiba S. Assessment of the plasma/serum IgG test to screen for periodontitis. J Dent Res. 2012 Dec;91(12):1190-5. doi: 10.1177/0022034512461796. Epub 2012 Sep 26.

Helpful Links

• Introduction site for the project supported by JSPS, and guiding the visitors to "Web-based Management System for Data of Periodontal Infection". Notice: this site is written in Japanese.

Study record dates

Study Major Dates

• Study Start: January 1, 2007
• Primary Completion (Actual): November 1, 2009
• Study Completion (Actual): March 1, 2010

Study Registration Dates

• First Submitted: July 31, 2012
• First Submitted That Met QC Criteria: August 2, 2012
• First Posted (Estimate): August 7, 2012

Study Record Updates

• Last Update Posted (Estimate): August 7, 2012
• Last Update Submitted That Met QC Criteria: August 2, 2012
• Last Verified: August 1, 2012

More Information

Terms related to this study

• Keywords: IgG titer; periodontopathic bacteria
• Additional Relevant MeSH Terms: Stomatognathic Diseases; Periodontal Diseases; Mouth Diseases; Periodontitis
• Other Study ID Numbers: OUGSMDPS-350&453; JSPS #18209061 & H19-Choju-008 (Other Grant/Funding Number: JSPS & Ministry of Health, Labor, and Welfare of Japan)