Study to Examine the Effects of ATP Supplementation on Muscle Excitability and Reaction Time During and Following a Repeated Sprint Bout

We hypothesize that adenosine-5'-triphosphate (ATP) supplementation will be able to enhance motor unit recruitment while simultaneously blunting the decline in power and reaction time that is typically seen during repetitive sprints. This will give researchers a better understanding of the mechanism by which ATP supplementation can augment power, especially under high fatiguing conditions.

Study Overview

• Status: Completed
• Conditions: Muscle Fatigue Caused by Repetitive Muscle Exercise
• Intervention / Treatment: Dietary supplement: Placebo; Dietary supplement: ATP

Detailed Description

Healthy participants of similar training status as previously tested will be enrolled into the study. The participants will be randomly assigned to either placebo supplementation or to 400 mg ATP per day for 14 days. The supplements will be taken in the morning and the last dose will be taken 30 min prior to testing on the last day. Testing will be performed at baseline and after the 14 days of supplementation. Ten successive sprints will be conducted on the Wingate apparatus as this has been previously demonstrated to decrease muscle excitability. After the sprints, muscle excitability, reaction time, and vertical jump power will be measured. In addition a blood sample will be obtained before and after supplementation and complete blood hematology and chemistry analyses will be performed. It is expected that ATP supplementation may help maintain muscle excitability and power after the successive sprint sessions.

• Study Type: Interventional
• Enrollment (Actual): 42
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United States
  • Florida
    • Tampa, Florida, United States, 33606
      • Jacob M Wilson, PhD

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 30 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Agree to eating a diet approved by a registered dietician consisting of the following by caloric percentages: 15-20% protein, 44-55% carbohydrate, and 25-30% fat.
• Must not have taken performance enhancing supplements for at least 3 months prior to the study start,
• Must be a non-smoker.
• Must not be taking amino acid supplements.
• Must not be using anabolic or catabolic hormones.
• Must not be taking any medications known to influence variables to be measured in the study.

Exclusion Criteria:

• Will not agree to eating a diet approved by a registered dietician consisting of the following by caloric percentages: 15-20% protein, 44-55% carbohydrate, and 25-30% fat.
• Has taken performance enhancing supplements for at least 3 months prior to the study start,
• Is currently a non-smoker.
• Is currently taking amino acid supplements.
• Is currently using anabolic or catabolic hormones.
• Is currently taking medications known to influence variables to be measured in the study.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Triple

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Placebo Comparator: Placebo; This arm will serve as the placebo supplementation.	Dietary supplement: Placebo; Other Names: Placebo Comparator
Active Comparator: ATP; This arm will serve as the treatment intervention, ATP	Dietary supplement: ATP; 400 mg of ATP; Other Names: Peak ATP

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Muscle Excitability	Muscle excitability will be measured using Delsys Wireless Electromyography (EMG) sensors.	Day 14

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Power	Muscle power will be measured using the Wingate and vertical jump power measures.	Day 14

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Reaction Time	Reaction time will be measured using a Dynavision apparatus.	Day 14
Blood Biochemical Analyses	Blood chemistries and hematology will be measured after the 14 days of supplementation.	Day 14

Collaborators and Investigators

• Sponsor: Metabolic Technologies Inc.
• Collaborators: University of Tampa
• Investigators: Study Director: John A Rathmacher, PhD, Metabolic Technologies Inc.

Study record dates

Study Major Dates

• Study Start: July 1, 2013
• Primary Completion (Actual): August 1, 2015
• Study Completion (Actual): August 1, 2015

Study Registration Dates

• First Submitted: March 18, 2014
• First Submitted That Met QC Criteria: March 19, 2014
• First Posted (Estimate): March 21, 2014

Study Record Updates

• Last Update Posted (Estimate): September 28, 2015
• Last Update Submitted That Met QC Criteria: September 24, 2015
• Last Verified: September 1, 2015

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Fatigue
• Other Study ID Numbers: MTI2013-CS04