- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT02699177
In Vivo Measurements of Nasal Ciliary Beat Frequency by Using Interferometry
- Assessment of a high speed video camera with a green light source for the measurement of ciliary beat frequency (CBF) in the nasal airways of patients.
- Assessments of the effect of drugs and other therapies on CBF using the study system.
- Comparison of results with standard methods such as ciliary brush biopsies
Study Overview
Status
Conditions
Intervention / Treatment
Detailed Description
Background Mucociliary clearance of the airways depends on ciliary movement by ciliated cells that cover the entire airway and the sinuses. In many airway diseases the mucociliary clearance is affected by injury caused by smoke, viruses, other infectious organisms or genetic diseases that are called Primary Ciliary Dyskinesia (PCD).
The diagnosis of PCD is based on a combination of clinical findings such as bronchiectasis, chronic sinusitis, chronic otitis media and immotile sperms.
In addition, in tests performed in the pulmonary laboratory nasal nitric oxide levels may be low.
Samples obtained by nasal brush biopsy can be analyzed and the ciliary movement recorded by video microscopy. Ciliary beat frequency (CBF) can be calculated in freshly isolated samples but also after growing the tissue in cell culture.
Furthermore, ciliated cells can be analyzed by electron microscopy to detect defects in the microscopic structure of the cilia.
The major problem encountered by all these assays is that they are performed under in vitro conditions and not in the natural environment of the cells where the acidity (pH), temperature, electrolytes etc. may be very different.
Moreover, genetic testing is limited to mutations known to cause PCD, ruling out mutations not yet discovered.
Therefore a method is needed to unequivocally measure ciliary movement in the natural environment.
Goal
- Assessment of a high speed video camera with a green light source for the measurement of ciliary beat frequency (CBF) in the nasal airways of patients.
- Assessments of the effect of drugs and other therapies on CBF using the study system.
Methods The protocol has been approved by the local Institutional Review Board (IRB) of the Hadassah Medical Center, Jerusalem, Israel
Equipment:
- High Speed High Video Microscope built to specifications needed for this study based on a commercially available high speed video system.
- The green light source (LED) used consists of a spot of 3mm diameter and has an energy of 10 milli Watt ( equal to 1Kilowatt/square meter)
- Video camera (manufactured by SONY Inc, Japan)
Patients:
Patients referred for ciliary brush biopsy will be asked to sign the informed consent and to be enrolled into the study.
Patients will be examined by having a high speed digital video camera placed close to the nasal cavity in order to photograph the area of interest. The camera lens will be off focus and the reflected light from the nasal mucosa will be recorded. This reflected light recording will be analyzed to obtain the CBF, heart rate and respiratory rate.
After the recording the patient will proceed to the nasal brush ciliary biopsy. The cells will be analyzed by a CBF will be calculated from several areas of the smear.
Safety measures:
All participating patients will wear a protective cover over their eyes during photography.
Study Type
Enrollment (Anticipated)
Contacts and Locations
Study Contact
- Name: David Shoseyov, MD
- Phone Number: 972-507874091
- Email: shoseyov@hadassah.org.il
Study Locations
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-
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Jerusalem, Israel, 91240
- Hadassah Medical Organization
-
Contact:
- David Shoseyov, MD
- Phone Number: 972-2-5844430/1
- Email: shoseyov@hadassah.org.il
-
Sub-Investigator:
- Reuven Tzabari, MD
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-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Sampling Method
Study Population
Description
Inclusion Criteria:
- Patients referred for Ciliary Brush Biopsy
Exclusion Criteria:
- Active nasal bleeding
Study Plan
How is the study designed?
Design Details
- Observational Models: Cohort
- Time Perspectives: Prospective
Cohorts and Interventions
Group / Cohort |
Intervention / Treatment |
---|---|
Suspected PCD but negative
CBF measurements:
|
CBF measurements
Other Names:
|
Suspected PCD but positive
CBF measurements:
|
CBF measurements
Other Names:
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Ciliary beat frequency (CBF) measured by high speed video microscopy
Time Frame: 5 minutes
|
Ex vivo measurements of CBF analysing high speed video records of nasal brush biopsies
|
5 minutes
|
Ciliary beat frequency (CBF) measured by interferometry
Time Frame: 5 minutes
|
In vivo measurements of CBF analysing the reflected light from the nasal cavity
|
5 minutes
|
Collaborators and Investigators
Sponsor
Investigators
- Principal Investigator: David Shoseyov, MD, Hadassah Medical Organization
Study record dates
Study Major Dates
Study Start
Primary Completion (Anticipated)
Study Completion (Anticipated)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Estimate)
Study Record Updates
Last Update Posted (Estimate)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Keywords
Additional Relevant MeSH Terms
Other Study ID Numbers
- 0366-15-HMO-CTIL
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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