Expression of DMBT1 in Colorectal Cancer Patients (DMBT1)

Modification of the Expression and the Localization of DMBT1 (Deleted in Malignant Brain Tumor 1) in Colorectal Cancer

Although colorectal cancer is a preventable and curable disease if early stage tumors are removed, it is still the fourth cause of cancer worldwide and the second leading cause of death in many industrialized countries. The 5-year survival is about 55% often due to a late detection. Then, the identification of sensitive and specific molecular markers is therefore a major challenge for early diagnosis and prognosis of this disease.

Preliminary work have reported variations in the expression of DMBT1 (deleted in malignant brain tumor 1), a glycoprotein co-secreted with mucins in the light of the glands, during several stages of colon carcinogenesis. The goal of this study is to study by mass spectrometry (MS), alterations in the repertoire of glycosylation of mucins from colorectal tumors of various stages, grades, and recurrence status.

Study Overview

• Status: Completed
• Conditions: Colorectal Cancer; DMBT1 Protein, Human
• Intervention / Treatment: Other: Detection of the expression of DMTB1 by immunohistochemistry

• Study Type: Observational
• Enrollment (Actual): 145

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

Individuals with colorectal carcinoma, with polyps or without colonic pathology

Description

Inclusion Criteria:

• for colon cancer: patients surgically treated for colorectal cancer at the Group of hospitals of the Catholic Institute of Lille.
• for adenomatous polyps : patients treated for adenomatous polyps at the Group of hospitals of the Catholic Institute of Lille.
• for controls: patients surgically treated for diverticular disease at the Group of hospitals of the Catholic Institute of Lille.

Exclusion Criteria:

• none

Study Plan

How is the study designed?

Number of groups / cohorts

3

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
Patients with colorectal cancer; Fresh tissue samples from individuals with colorectal carcinoma taken from the tumor as well as from resection margins will be used for the detection of a potential marker of carcinogenesis.	Other: Detection of the expression of DMTB1 by immunohistochemistry
Patients with adenomatous polyps; Fresh tissue samples from individuals with adenomatous polyps will be used for the detection of a potential marker of carcinogenesis.	Other: Detection of the expression of DMTB1 by immunohistochemistry
Control patients; Fresh tissue samples from individuals without colorectal carcinoma will be used as control.	Other: Detection of the expression of DMTB1 by immunohistochemistry

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Semi-quantitative determination of the histological expression of DMBT1	The expression of DMTB1 will be determined by observation of the slides after immunohistochemistry using an anti-DMBT1 antibody	at baseline
Determination of the histological localisation of DMBT1	The localisation of DMTB1 will be determined by observation of slides after immunohistochemistry using an anti-DMBT1 antibody	at baseline

Collaborators and Investigators

• Sponsor: Lille Catholic University
• Investigators: Principal Investigator: Adriana Mihalache, MD, PhD, GHICL

Study record dates

Study Major Dates

• Study Start: June 1, 2013
• Primary Completion (Actual): June 1, 2015
• Study Completion (Actual): November 1, 2015

Study Registration Dates

• First Submitted: September 12, 2016
• First Submitted That Met QC Criteria: September 12, 2016
• First Posted (Estimate): September 15, 2016

Study Record Updates

• Last Update Posted (Estimate): September 15, 2016
• Last Update Submitted That Met QC Criteria: September 12, 2016
• Last Verified: September 1, 2016

More Information

Terms related to this study

• Keywords: Colorectal cancer; DMBT1 expression
• Additional Relevant MeSH Terms: Digestive System Diseases; Neoplasms; Neoplasms by Site; Gastrointestinal Neoplasms; Digestive System Neoplasms; Gastrointestinal Diseases; Colonic Diseases; Intestinal Diseases; Intestinal Neoplasms; Rectal Diseases; Colorectal Neoplasms
• Other Study ID Numbers: RT-06

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO