Effect of Vitamin D Status on Clinical Pregnancy Rates Following Intra Cytoplasmic Sperm Injection

All patients will undergo in vitro fertilization.

Serum 25 (OH) D samples will be collected within1 week before oocyte retrieval.

Whole blood specimens will be collected in serum separator tubes using acceptable medical techniques to avoid hemolysis.

Ultrasound guided fresh embryo transfer will be performed on day 5 after fertilization.

clinical pregnancy will be confirmed by observation of intrauterine sac visible on ultrasound performed 4-5 weeks after embryo transfer.

Study Overview

• Status: Unknown
• Conditions: Infertility

Detailed Description

PATIENTS AND METHODS

Protocol Outline:

The Study:

Study site:

Ain Shams University Maternity Hospital.

Study Design:

A prospective Cohort study.

Study setting:

The study will be conducted at Ain Shams University Maternity Hospital.

Study population:

The study will include all women planned to undergo IVF due to unexplained infertility.

Methodology:

The study will be approved by the hospital ethics committee.

All included women will undergo the following:

• History taking.
• General and abdominal examination.
• Vaginal examination and assessment
• Pelvic ultrasound.
• Routine laboratory investigations including complete blood picture, hematocrit, liver and kidney function tests and coagulation profile.
• Preoperative senior anesthetist assessment.
• Hysteroscopy.

All patients will undergo IVF.

Serum 25(OH)D samples will be collected within1 week before oocyte retrieval.

Whole blood specimens will be collected in serum separator tubes using acceptable medical techniques to avoid hemolysis.

Ultrasound-guided fresh embryo transfer will be performed on day 5 after fertilization.

clinical pregnancy will be confirmed by observation of intrauterine sac visible on ultrasound performed 4-5 weeks after embryo transfer.

Measurement of Outcomes

1. serum 25 (OH) D levels.

2. Number of oocyte.

   Most studies put the optimal number of retrieved oocytes between 10 and 15.

3. The implantation rate:

   Calculated as the number of gestational sacs observed by ultrasound divided by the number of embryos transferred, multiplied by 100.

4. Number of gestational sac.

• Study Type: Observational
• Enrollment (Anticipated): 240

Contacts and Locations

• Study Contact: Name: Mohammed Samy, MD; Phone Number: +20100 194 7488; Email: dr_hend_kamal@yahoo.com

Study Locations

• Egypt
  • Abbassia
    • Cairo, Abbassia, Egypt, 11566
      • Recruiting
      • Ain shams university maternity hospital
      • Contact: Moh Samy, MD; Phone Number: 201002554281; Email: dr_hend_kamal@yahoo.com
      • Contact: Mohammed Samy, MD; Phone Number: +20100 194 7488

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years to 35 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Female

• Sampling Method: Probability Sample

Study Population

• Age between 20 and 35 years old.
• Body mass index between 20 - 30.
• Unexplained infertility .
• Follicle-stimulating hormone level 12 IU/L or lower (baseline level, cycle day 3).
• Normal hysteroscopy .
• Fresh embryo transfer of 2 or more blastocysts stage.
• Embryo transfer on day 5 after fertilization

Description

Inclusion Criteria:

• Age between 20 and 35 years old.
• Body mass index between 20 - 30.
• Unexplained infertility .
• Follicle-stimulating hormone level 12 IU/L or lower (baseline level, cycle day 3).
• Normal hysteroscopy ( healthy uterus with no fibroids or other growths)
• Fresh embryo transfer of 2 or more blastocysts stage.
• Embryo transfer on day 5 after fertilization

Exclusion Criteria:

• Women younger than 20 years old or older than 35 years old.
• Previous one or more failed IVF.
• Uncorrected congenital or acquired uterine anomalies.
• Other causes of infertility rather than unexplained infertility.
• Less than 2 embryo transfer, cleavage stager frozen embryo transfer.

Study Plan

How is the study designed?

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
pregnancy	4-5 weeks

Collaborators and Investigators

• Sponsor: Ain Shams Maternity Hospital

Study record dates

Study Major Dates

• Study Start: March 1, 2016
• Primary Completion (Anticipated): March 1, 2017
• Study Completion (Anticipated): December 1, 2017

Study Registration Dates

• First Submitted: November 29, 2016
• First Submitted That Met QC Criteria: December 6, 2016
• First Posted (Estimate): December 9, 2016

Study Record Updates

• Last Update Posted (Estimate): December 9, 2016
• Last Update Submitted That Met QC Criteria: December 6, 2016
• Last Verified: December 1, 2016

More Information

Terms related to this study

• Keywords: Vitamin D following intra cytoplasmic sperm injection
• Additional Relevant MeSH Terms: Infertility
• Other Study ID Numbers: 6789 (CTEP)